BLASTX 2.0.8 [Jan-05-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 14VII36R
(680 letters)
Database: Non-redundant GenBank CDS
translations+PDB+SwissProt+SPupdate+PIR
369,800 sequences; 113,023,754 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gi|1082407|pir||A55314 glycine--tRNA ligase (EC 6.1.1.14) precur... 141 3e-33
gi|493066 (U09510) glycyl-tRNA synthetase [Homo sapiens] 141 3e-33
gi|730868|sp|P41250|SYG_HUMAN GLYCYL-TRNA SYNTHETASE (GLYCINE--T... 141 3e-33
gi|2132408|pir||S69067 probable glycine--tRNA ligase (EC 6.1.1.1... 137 9e-32
gi|1351154|sp|Q10039|SYG_CAEEL PROBABLE GLYCYL-TRNA SYNTHETASE (... 129 1e-29
gi|586057|sp|P38088|SYG_YEAST GLYCYL-TRNA SYNTHETASE (GLYCINE--T... 129 2e-29
gi|1351153|sp|Q04451|SYG_BOMMO GLYCYL-TRNA SYNTHETASE (GLYCINE--... 127 6e-29
gi|3334349|sp|O23627|SYG_ARATH GLYCYL-TRNA SYNTHETASE (GLYCINE--... 125 3e-28
gi|422391|pir||A46636 glycine--tRNA ligase (EC 6.1.1.14) - silkworm 122 2e-27
gi|1711632|sp|Q10179|SYG_SCHPO PUTATIVE GLYCYL-TRNA SYNTHETASE (... 122 3e-27
gi|2500998|sp|Q57681|SYG_METJA PROBABLE GLYCYL-TRNA SYNTHETASE (... 120 1e-26
gi|3258043|dbj|BAA30726| (AP000006) 570aa long hypothetical glyc... 118 3e-26
gi|3334352|sp|O27874|SYG_METTH PROBABLE GLYCYL-TRNA SYNTHETASE (... 110 8e-24
gi|3242765 (AC005154) glycyl-tRNA synthetase; similar to protein... 106 1e-22
gi|3334354|sp|O29346|SYG_ARCFU PROBABLE GLYCYL-TRNA SYNTHETASE (... 92 3e-18
gi|2078043|emb|CAB08466| (Z95208) glyS [Mycobacterium tuberculosis] 81 7e-15
gi|2147427|pir||S58522 glycyl-tRNA synthetase - Thermus thermoph... 78 4e-14
gi|2392169|pdb|1ATI|A Chain A, Crystal Structure Of Glycyl-Trna ... 77 1e-13
gi|2500997|sp|P75425|SYG_MYCPN GLYCYL-TRNA SYNTHETASE (GLYCINE--... 77 1e-13
gi|2829475|sp|P56206|SYG_THETH GLYCYL-TRNA SYNTHETASE (GLYCINE--... 77 1e-13
gi|2688266 (AE001142) glycyl-tRNA synthetase (glyS) # [Borrelia ... 71 7e-12
gi|1351155|sp|P47493|SYG_MYCGE GLYCYL-TRNA SYNTHETASE (GLYCINE--... 71 7e-12
gi|3322974 (AE001241) glycyl-tRNA synthetase (glyS) [Treponema p... 70 1e-11
gi|2664208|emb|CAA10903| (AJ222643) Glycyl-tRNA synthetase [Ther... 68 5e-11
gi|4388929|pdb|1B76|A Chain A, Glycyl-Trna Synthetase From Therm... 64 1e-09
gi|2392770 (AC002534) similar to glycyl tRNA synthetase [Arabido... 40 0.014
gi|1169007|sp|Q03727|COMA_STRPN TRANSPORT ATP-BINDING PROTEIN CO... 31 9.0
gi|98020|pir||A39203 comA protein - Streptococcus pneumoniae 31 9.0
>gi|1082407|pir||A55314 glycine--tRNA ligase (EC 6.1.1.14) precursor - human
>gi|1311463|dbj|BAA06338| (D30658) glycyl tRNA
synthetase [Homo sapiens]
Length = 739
Score = 141 bits (353), Expect = 3e-33
Identities = 78/182 (42%), Positives = 112/182 (60%), Gaps = 11/182 (6%)
Query: 548 RRETMLRKRXFIASSFSIYGGVSGLYDLGPLGTSLKNQILRVFRDIFCKD--LFEIESTI 375
+ E L++R F +F+IYGGVSGLYD GP+G +LKN I++ +R F ++ + EI+ T+
Sbjct: 123 KMEDTLKRRFFYDQAFAIYGGVSGLYDFGPVGCALKNNIIQTWRQHFIQEEQILEIDCTM 182
Query: 374 LTPYQVLKASGHIDKFCDILLFDSVNGECYRADHYXXXXXX--------XXXXXXEIDKM 219
LTP VLK SGH+DKF D ++ D NGEC+RADH E++ +
Sbjct: 183 LTPEPVLKTSGHVDKFADFMVKDVKNGECFRADHLLKAHLQKLMSDKKCSVEKKSEMESV 242
Query: 218 IEKLECMSLEEIDKVVEDYKILSK-XKNTLSKSQRFNLMFETFVGPKKKNXIYXRPETAQ 42
+ +L+ +E+ + +Y + S N LS FNLMF+TF+GP Y RPETAQ
Sbjct: 243 LAQLDNYGQQELADLFVNYNVKSPITGNDLSPPVSFNLMFKTFIGPGGNMPGYLRPETAQ 302
Query: 41 GQFVNXXXVYELN 3
G F+N + E N
Sbjct: 303 GIFLNFKRLLEFN 315
>gi|493066 (U09510) glycyl-tRNA synthetase [Homo sapiens]
Length = 685
Score = 141 bits (353), Expect = 3e-33
Identities = 78/182 (42%), Positives = 112/182 (60%), Gaps = 11/182 (6%)
Query: 548 RRETMLRKRXFIASSFSIYGGVSGLYDLGPLGTSLKNQILRVFRDIFCKD--LFEIESTI 375
+ E L++R F +F+IYGGVSGLYD GP+G +LKN I++ +R F ++ + EI+ T+
Sbjct: 69 KMEDTLKRRFFYDQAFAIYGGVSGLYDFGPVGCALKNNIIQTWRQHFIQEEQILEIDCTM 128
Query: 374 LTPYQVLKASGHIDKFCDILLFDSVNGECYRADHYXXXXXX--------XXXXXXEIDKM 219
LTP VLK SGH+DKF D ++ D NGEC+RADH E++ +
Sbjct: 129 LTPEPVLKTSGHVDKFADFMVKDVKNGECFRADHLLKAHLQKLMSDKKCSVEKKSEMESV 188
Query: 218 IEKLECMSLEEIDKVVEDYKILSK-XKNTLSKSQRFNLMFETFVGPKKKNXIYXRPETAQ 42
+ +L+ +E+ + +Y + S N LS FNLMF+TF+GP Y RPETAQ
Sbjct: 189 LAQLDNYGQQELADLFVNYNVKSPITGNDLSPPVSFNLMFKTFIGPGGNMPGYLRPETAQ 248
Query: 41 GQFVNXXXVYELN 3
G F+N + E N
Sbjct: 249 GIFLNFKRLLEFN 261
>gi|730868|sp|P41250|SYG_HUMAN GLYCYL-TRNA SYNTHETASE (GLYCINE--TRNA LIGASE) (GLYRS) >gi|600727
(U09587) glycyl-tRNA synthetase [Homo sapiens]
Length = 685
Score = 141 bits (353), Expect = 3e-33
Identities = 78/182 (42%), Positives = 112/182 (60%), Gaps = 11/182 (6%)
Query: 548 RRETMLRKRXFIASSFSIYGGVSGLYDLGPLGTSLKNQILRVFRDIFCKD--LFEIESTI 375
+ E L++R F +F+IYGGVSGLYD GP+G +LKN I++ +R F ++ + EI+ T+
Sbjct: 69 KMEDTLKRRFFYDQAFAIYGGVSGLYDFGPVGCALKNNIIQTWRQHFIQEEQILEIDCTM 128
Query: 374 LTPYQVLKASGHIDKFCDILLFDSVNGECYRADHYXXXXXX--------XXXXXXEIDKM 219
LTP VLK SGH+DKF D ++ D NGEC+RADH E++ +
Sbjct: 129 LTPEPVLKTSGHVDKFADFMVKDVKNGECFRADHLLKAHLQKLMSDKKCSVEKKSEMESV 188
Query: 218 IEKLECMSLEEIDKVVEDYKILSK-XKNTLSKSQRFNLMFETFVGPKKKNXIYXRPETAQ 42
+ +L+ +E+ + +Y + S N LS FNLMF+TF+GP Y RPETAQ
Sbjct: 189 LAQLDNYGQQELADLFVNYNVKSPITGNDLSPPVSFNLMFKTFIGPGGNMPGYLRPETAQ 248
Query: 41 GQFVNXXXVYELN 3
G F+N + E N
Sbjct: 249 GIFLNFKRLLEFN 261
>gi|2132408|pir||S69067 probable glycine--tRNA ligase (EC 6.1.1.14) - yeast (Saccharomyces
cerevisiae) >gi|1230687 (U51033) Highly similar to
Glycyl-tRNA synthetase (Swiss Prot. accession numbers
P41250 and P38088) [Saccharomyces cerevisiae]
Length = 618
Score = 137 bits (341), Expect = 9e-32
Identities = 74/186 (39%), Positives = 115/186 (61%), Gaps = 11/186 (5%)
Query: 560 NKAERRETMLRKRXFIASSFSIYGGVSGLYDLGPLGTSLKNQILRVFRDIFC--KDLFEI 387
++ ++ E+ LR+R F SF IYGGVSGL+DLGP G L+N ++R++R+ F +++ ++
Sbjct: 7 SERDKLESTLRRRFFYTPSFEIYGGVSGLFDLGPPGCQLQNNLIRLWREHFIMEENMLQV 66
Query: 386 ESTILTPYQVLKASGHIDKFCDILLFDSVNGECYRADHYXXXXXXXXXXXXEI------- 228
+ +LTPY VLK SGH+DKF D + + GE YRADH ++
Sbjct: 67 DGPMLTPYDVLKTSGHVDKFTDWMCRNPKTGEYYRADHLIEQTLKKRLLDKDVNPQDMKN 126
Query: 227 -DKMIEKLECMSLEEIDKVVEDYKILSKXKN-TLSKSQRFNLMFETFVGPKKKNXIYXRP 54
+K++ ++ S E++ V+++Y I N L FNLMFET +G + + RP
Sbjct: 127 MEKILTTIDGFSGPELNLVMQEYNINDPVTNDVLDALTSFNLMFETKIGASGQLKAFLRP 186
Query: 53 ETAQGQFVNXXXVYELN 3
ETAQGQF+N + E+N
Sbjct: 187 ETAQGQFLNFNKLLEIN 203
>gi|1351154|sp|Q10039|SYG_CAEEL PROBABLE GLYCYL-TRNA SYNTHETASE (GLYCINE--TRNA LIGASE) (GLYRS)
>gi|727447 (U23412) coded for by C. elegans cDNA
yk46g2.5; coded for by C. elegans cDNA yk46g2.3;
similar to glycyl-tRNA synthetase [Caenorhabditis
elegans]
Length = 746
Score = 129 bits (322), Expect = 1e-29
Identities = 74/180 (41%), Positives = 107/180 (59%), Gaps = 11/180 (6%)
Query: 542 ETMLRKRXFIASSFSIYGGVSGLYDLGPLGTSLKNQILRVFRDIFCKD--LFEIESTILT 369
E +L++R F SF+IYGGV+GLYD GP+G SLK +L+ +R F + + E++ T LT
Sbjct: 139 EDLLKRRFFYDQSFAIYGGVTGLYDFGPMGCSLKANMLQEWRKHFILEEGMLEVDCTSLT 198
Query: 368 PYQVLKASGHIDKFCDILLFDSVNGECYRADHYXXXX--------XXXXXXXXEIDKMIE 213
P VLKASGH+D+F D ++ D NGEC+RADH + ++
Sbjct: 199 PEPVLKASGHVDRFADWMVKDMKNGECFRADHLIKNSIEKLLNDKKTSAAVKQDGQDVLA 258
Query: 212 KLECMSLEEIDKVVEDYKILSK-XKNTLSKSQRFNLMFETFVGPKKKNXIYXRPETAQGQ 36
+LE +++ +V+ + S N L++ FNLMF T +GP + RPETAQG
Sbjct: 259 RLEGFDNKDMHEVITRFNFKSPITGNDLTEPIAFNLMFPTQIGPTGDFKAFLRPETAQGI 318
Query: 35 FVNXXXVYELN 3
FVN + E N
Sbjct: 319 FVNFKRLLEFN 329
>gi|586057|sp|P38088|SYG_YEAST GLYCYL-TRNA SYNTHETASE (GLYCINE--TRNA LIGASE) (GLYRS)
>gi|1078212|pir||S48285 probable glycine--tRNA ligase
(EC 6.1.1.14) GRS1 - yeast (Saccharomyces cerevisiae)
>gi|536402|emb|CAA85078| (Z35990) ORF YBR121c
[Saccharomyces cerevisiae] >gi|565058|emb|CAA55623|
(X78993) probable transfer RNA-Gly synthetase
[Saccharomyces cerevisiae]
Length = 667
Score = 129 bits (321), Expect = 2e-29
Identities = 83/188 (44%), Positives = 119/188 (63%), Gaps = 46/188 (24%)
Query: 566 PMNKAERRETMLRKRXFIASSFSIYGGVSGLYDLGPLGTSLKNQILRVFRDIFC--KDLF 393
P N+ E+ E++LR R F A +F +YGGVSGLYD GP G + +N I+ +R F +D+
Sbjct: 14 PFNR-EQLESVLRGRFFYAPAFDLYGGVSGLYDYGPPGCAFQNNIIDAWRKHFILEEDML 72
Query: 392 EIESTILTPYQVLKASGHIDKFCDILLFDSVNGECYRADHY------------------- 270
E++ T+LTPY+VLK SGH+DKF D + D GE +RADH
Sbjct: 73 EVDCTMLTPYEVLKTSGHVDKFSDWMCRDLKTGEIFRADHLVEEVLEARLKGDQEARGLV 132
Query: 269 --XXXXXXXXXXXXEIDKMIEKLECMSLEEIDKVVEDY-KILSK---------------- 147
+ K +++++ + L+ D VV++Y +IL+K
Sbjct: 133 EDANAAAKDDAEKKKRKKKVKQIKAVKLD--DDVVKEYEEILAKIDGYSGPELGELMEKY 190
Query: 146 ------XKNTLSKSQRFNLMFETFVGPKKKNXIYXRPETAQGQFVNXXXVYELN 3
TL + FNLMFET +GP + Y RPETAQGQF+N + E N
Sbjct: 191 DIGNPVTGETLESPRAFNLMFETAIGPSGQLKGYLRPETAQGQFLNFNKLLEFN 244
>gi|1351153|sp|Q04451|SYG_BOMMO GLYCYL-TRNA SYNTHETASE (GLYCINE--TRNA LIGASE) (GLYRS) >gi|665680
(L08106) glycyl-tRNA synthetase [Bombyx mori]
Length = 680
Score = 127 bits (317), Expect = 6e-29
Identities = 68/182 (37%), Positives = 112/182 (61%), Gaps = 11/182 (6%)
Query: 548 RRETMLRKRXFIASSFSIYGGVSGLYDLGPLGTSLKNQILRVFRDIFC--KDLFEIESTI 375
+ E ++++R F SF+IYGG++G +D GP+G +LK+ ++ +++ F + + E+E +I
Sbjct: 69 KMEDLIKRRFFYDQSFAIYGGITGQFDFGPMGCALKSNMIHLWKKFFILQEQMLEVECSI 128
Query: 374 LTPYQVLKASGHIDKFCDILLFDSVNGECYRADH--------YXXXXXXXXXXXXEIDKM 219
LTP VLKASGH+++F D++ D GEC+R DH EI+ +
Sbjct: 129 LTPEPVLKASGHVERFADLMTKDIKTGECFRLDHLIKGHLEKIKSDKNTKIELKAEIEDI 188
Query: 218 IEKLECMSLEEIDKVVEDYKILSKXK-NTLSKSQRFNLMFETFVGPKKKNXIYXRPETAQ 42
+ KL+ M+ +E+ +++ +++ S N L+ FNLMF T +GP + RPETAQ
Sbjct: 189 LIKLDGMNADEMSALMKRFEMKSPISGNDLTPPIEFNLMFNTQIGPSGLVKGFLRPETAQ 248
Query: 41 GQFVNXXXVYELN 3
G FVN + E N
Sbjct: 249 GIFVNFKRLLEFN 261
>gi|3334349|sp|O23627|SYG_ARATH GLYCYL-TRNA SYNTHETASE (GLYCINE--TRNA LIGASE) (GLYRS)
>gi|2564215|emb|CAA05162| (AJ002062) glycyl-tRNA
synthetase [Arabidopsis thaliana]
Length = 729
Score = 125 bits (311), Expect = 3e-28
Identities = 72/177 (40%), Positives = 102/177 (56%), Gaps = 12/177 (6%)
Query: 533 LRKRXFIASSFSIYGGVSGLYDLGPLGTSLKNQILRVFRDIFC--KDLFEIESTILTPYQ 360
L +R F SF IY GV+GL+D GP G ++K+ +L +R F +++ E++ +TP
Sbjct: 124 LERRLFYIPSFKIYSGVAGLFDYGPPGCAIKSNVLSFWRQHFILEENMLEVDCPCVTPEV 183
Query: 359 VLKASGHIDKFCDILLFDSVNGECYRADH---------YXXXXXXXXXXXXEIDKMIEKL 207
VLKASGH+DKF D+++ D G CYRADH E+ ++ +
Sbjct: 184 VLKASGHVDKFTDLMVKDEKTGTCYRADHLLKDYCTEKLEKDLTISAEKAAELKDVLAVM 243
Query: 206 ECMSLEEIDKVVEDYKILS-KXKNTLSKSQRFNLMFETFVGPKKKNXIYXRPETAQGQFV 30
E S E++ + +Y I + KN LS FNLMF+T +GP Y RPETAQG FV
Sbjct: 244 EDFSPEQLGAKIREYGITAPDTKNPLSDPYPFNLMFQTSIGPSGLIPGYMRPETAQGIFV 303
Query: 29 NXXXVYELN 3
N +Y N
Sbjct: 304 NFKDLYYYN 312
>gi|422391|pir||A46636 glycine--tRNA ligase (EC 6.1.1.14) - silkworm
Length = 687
Score = 122 bits (303), Expect = 2e-27
Identities = 69/182 (37%), Positives = 112/182 (60%), Gaps = 17/182 (9%)
Query: 548 RRETMLRKRXFIASSFS------IYGGVSGLYDLGPLGTSLKNQILRVFRDIFC--KDLF 393
+ E ++++R F SFS IYGG++G +D GP+G +LK+ ++ +++ F + +
Sbjct: 69 KMEDLIKRRFFYDQSFSMTSRFAIYGGITGQFDFGPMGCALKSNMIHLWKKFFILQEQML 128
Query: 392 EIESTILTPYQVLKASGHIDKFCDILLFDSVNGECYRADH--------YXXXXXXXXXXX 237
E+E +ILTP VLKASGH+++F D++ D GEC+R DH
Sbjct: 129 EVECSILTPEPVLKASGHVERFADLMTKDIKTGECFRLDHLIKGHLEKIKSDKNTKIELK 188
Query: 236 XEIDKMIEKLECMSLEEIDKVVEDYKILSKXK-NTLSKSQRFNLMFETFVGPKKKNXIYX 60
EI+ ++ KL+ M+ +E+ +++ +++ S N L+ FNLMF T +GP +
Sbjct: 189 AEIEDILIKLDGMNADEMSALMKRFEMKSPISGNDLTPPIEFNLMFNTQIGPSGLVKGFL 248
Query: 59 RPETAQGQFVNXXXVYELN 3
RPETAQG FVN + E N
Sbjct: 249 RPETAQGIFVNFKRLLEFN 267
>gi|1711632|sp|Q10179|SYG_SCHPO PUTATIVE GLYCYL-TRNA SYNTHETASE (GLYCINE--TRNA LIGASE) (GLYRS)
>gi|1182050|emb|CAA93301.1| (Z69369) glycyl tRNA
synthetase [Schizosaccharomyces pombe]
Length = 652
Score = 122 bits (302), Expect = 3e-27
Identities = 75/180 (41%), Positives = 114/180 (62%), Gaps = 44/180 (24%)
Query: 542 ETMLRKRXFIASSFSIYGGVSGLYDLGPLGTSLKNQILRVFRDIFC--KDLFEIESTILT 369
E +++KR F + SF IYGG+SGLYD GP G++L++ ++ ++R F + + E++ ++LT
Sbjct: 16 EELMKKRFFFSPSFQIYGGISGLYDYGPPGSALQSNLVDIWRKHFVIEESMLEVDCSMLT 75
Query: 368 PYQVLKASGHIDKFCDILLFDSVNGECYRADHYXXXXXXX-------------------X 246
P++VLK SGH+DKF D + D GE +RADH
Sbjct: 76 PHEVLKTSGHVDKFADWMCKDPATGEIFRADHLVEEVLEARLKGDKEARGQNSNDQPEES 135
Query: 245 XXXXEIDKMIEKLECMSLEEIDKVVEDYK-ILSKXKN----------------------T 135
+ K ++++ L+ DK VE+Y+ IL++ N
Sbjct: 136 DDKKKRKKKVKEIRATRLD--DKTVEEYEFILAQIDNYDGDQLGELMKKYDIRNPATNGE 193
Query: 134 LSKSQRFNLMFETFVGPKKKNXIYXRPETAQGQFVNXXXVYELN 3
L ++FNLMFET +GP Y RPETAQGQF+N + E N
Sbjct: 194 LETPRQFNLMFETQIGPSGGLKGYLRPETAQGQFLNFSRLLEFN 237
>gi|2500998|sp|Q57681|SYG_METJA PROBABLE GLYCYL-TRNA SYNTHETASE (GLYCINE--TRNA LIGASE) (GLYRS)
>gi|2127942|pir||E64328 glycine--tRNA ligase (EC
6.1.1.14) - Methanococcus jannaschii >gi|1499005
(U67478) glycyl-tRNA synthetase (glyS) [Methanococcus
jannaschii]
Length = 577
Score = 120 bits (297), Expect = 1e-26
Identities = 70/174 (40%), Positives = 104/174 (59%), Gaps = 4/174 (2%)
Query: 551 ERRETMLRKRXFIASSFSIYGGVSGLYDLGPLGTSLKNQILRVFRD--IFCKDLFEIEST 378
E+ + ++R ++ SSF IYGG++G D GPLG LKN I+ FR+ I + +EIES
Sbjct: 7 EKIMDLAKRRGYLWSSFEIYGGIAGFVDYGPLGCLLKNNIISKFREQYIIKEGFYEIESP 66
Query: 377 ILTPYQVLKASGHIDKFCDILLFDSVNGECYRADHYXXXXXXXXXXXXEIDKMIE-KLEC 201
+TPY+VLKASGH+D F D ++ E +RADH I++ ++ E
Sbjct: 67 TVTPYEVLKASGHVDNFTDPIVECKNCLESFRADH-------------LIEEFVDVDTEG 113
Query: 200 MSLEEIDKVVEDYKI-LSKXKNTLSKSQRFNLMFETFVGPKKKNXIYXRPETAQGQFV 30
+L+E+D+++ + I K L + ++FNLMF T +GP K Y RPETAQG F+
Sbjct: 114 KTLKELDELIRKHNIRCPKCGGELGEVKKFNLMFVTSIGPGGKRTGYMRPETAQGIFI 171
>gi|3258043|dbj|BAA30726| (AP000006) 570aa long hypothetical glycyl-tRNA synthetase
[Pyrococcus horikoshii]
Length = 570
Score = 118 bits (294), Expect = 3e-26
Identities = 71/178 (39%), Positives = 103/178 (56%), Gaps = 3/178 (1%)
Query: 560 NKAERRETMLRKRXFIASSFSIYGGVSGLYDLGPLGTSLKNQILRVFRDIFCKD-LFEIE 384
+K E + ++R+R F SF IYGG G YD GPLG ++K +I R R+ F ++ FEIE
Sbjct: 6 DKYEYLQDLMRRRGFAWGSFEIYGGSRGFYDYGPLGATIKRKIERKIREAFIREGFFEIE 65
Query: 383 STILTPYQVLKASGHIDKFCDILLFDSVNGECYRADHYXXXXXXXXXXXXEIDKMIE-KL 207
+ +TP QV ASGH++KF D ++ G +RADH I++ + +
Sbjct: 66 TPDITPEQVFIASGHVEKFVDPVVECKKCGMRFRADH-------------LIEEFLGIDV 112
Query: 206 ECMSLEEIDKVVEDYKI-LSKXKNTLSKSQRFNLMFETFVGPKKKNXIYXRPETAQGQFV 30
E S EE+ +++ ++ + + LS FNLMFET++GP K Y RPETAQG FV
Sbjct: 113 EGKSAEEMSRIIREHGLKCPECGGELSDVFYFNLMFETYIGPYKDKKAYLRPETAQGIFV 172
Query: 29 N 27
N
Sbjct: 173 N 173
>gi|3334352|sp|O27874|SYG_METTH PROBABLE GLYCYL-TRNA SYNTHETASE (GLYCINE--TRNA LIGASE) (GLYRS)
>gi|2622981 (AE000937) glycyl-tRNA synthetase
[Methanobacterium thermoautotrophicum]
Length = 565
Score = 110 bits (273), Expect = 8e-24
Identities = 66/174 (37%), Positives = 96/174 (54%), Gaps = 3/174 (1%)
Query: 551 ERRETMLRKRXFIASSFSIYGGVSGLYDLGPLGTSLKNQILRVFRDIFC--KDLFEIEST 378
ER T+ RKR F+ SSF IY GV+G D GPLG +LKN+I+ +R+ + + +EIES
Sbjct: 7 ERTMTVARKRGFLWSSFEIYSGVAGFVDYGPLGATLKNKIMNRWREFYVVREGFYEIESP 66
Query: 377 ILTPYQVLKASGHIDKFCDILLFDSVNGECYRADHYXXXXXXXXXXXXEIDKMIEKLECM 198
+ P + LKASGH+D F D + + YRADH D +E +
Sbjct: 67 TIMPEEALKASGHVDHFNDPMTQCKECMDVYRADHIIE------------DATGRDVEGL 114
Query: 197 SLEEIDKVVEDYKI-LSKXKNTLSKSQRFNLMFETFVGPKKKNXIYXRPETAQGQFV 30
+E+ +++ D I + L+ +NLMF+T +G + K Y RPETAQG F+
Sbjct: 115 ENQELTEIISDEGIRCPRCGGHLTHVWSYNLMFQTLIGARGKKTGYLRPETAQGIFI 171
>gi|3242765 (AC005154) glycyl-tRNA synthetase; similar to protein A55314
(PID:g1082407) [Homo sapiens]
Length = 245
Score = 106 bits (263), Expect = 1e-22
Identities = 49/92 (53%), Positives = 68/92 (73%), Gaps = 2/92 (2%)
Query: 548 RRETMLRKRXFIASSFSIYGGVSGLYDLGPLGTSLKNQILRVFRDIFCKD--LFEIESTI 375
+ E L++R F +F+IYGGVSGLYD GP+G +LKN I++ +R F ++ + EI+ T+
Sbjct: 123 KMEDTLKRRFFYDQAFAIYGGVSGLYDFGPVGCALKNNIIQTWRQHFIQEEQILEIDCTM 182
Query: 374 LTPYQVLKASGHIDKFCDILLFDSVNGECYRADH 273
LTP VLK SGH+DKF D ++ D NGEC+RADH
Sbjct: 183 LTPEPVLKTSGHVDKFADFMVKDVKNGECFRADH 216
>gi|3334354|sp|O29346|SYG_ARCFU PROBABLE GLYCYL-TRNA SYNTHETASE (GLYCINE--TRNA LIGASE) (GLYRS)
>gi|2649683 (AE001040) glycyl-tRNA synthetase (glyS)
[Archaeoglobus fulgidus]
Length = 570
Score = 92.1 bits (225), Expect = 3e-18
Identities = 58/170 (34%), Positives = 85/170 (49%), Gaps = 2/170 (1%)
Query: 536 MLRKRXFIASSFSIYGGVSGLYDLGPLGTSLKNQILRVFRDIFC--KDLFEIESTILTPY 363
ML +R F+ SF IYGG++G D PLG +L+ +I ++R F + EI++ +
Sbjct: 9 MLIRRGFLWQSFEIYGGMAGFIDYAPLGNNLRRKIENIWRKYFVINERAAEIDTPTIGIE 68
Query: 362 QVLKASGHIDKFCDILLFDSVNGECYRADHYXXXXXXXXXXXXEIDKMIEKLECMSLEEI 183
+V ASGH F D+ + G YRADHY +D+ +E ++
Sbjct: 69 EVFIASGHATSFTDVAIECENCGRVYRADHYVKEKLGIE-----VDETVEAVK------- 116
Query: 182 DKVVEDYKILSKXKNTLSKSQRFNLMFETFVGPKKKNXIYXRPETAQGQFVN 27
+V+E Y + + NLMF T +GP K Y RPETAQG FV+
Sbjct: 117 -EVMEVYDLKCECGGRFKDPAPMNLMFSTTIGPGKGKKGYLRPETAQGMFVD 167
>gi|2078043|emb|CAB08466| (Z95208) glyS [Mycobacterium tuberculosis]
Length = 463
Score = 81.1 bits (197), Expect = 7e-15
Identities = 58/174 (33%), Positives = 87/174 (49%), Gaps = 6/174 (3%)
Query: 536 MLRKRXFIASSFSIYGGVSGLYDLGPLGTSLKNQILRVF-RDIFC--KDLFEIESTILTP 366
+ ++R F+ S IYGG +D GPLG LK I R + R + D+ I+S+I+ P
Sbjct: 15 LAKRRGFVYPSGEIYGGTKSAWDYGPLGVELKENIKRQWWRSVVTGRDDVVGIDSSIILP 74
Query: 365 YQVLKASGHIDKFCDILLFDSVNGECYRADHYXXXXXXXXXXXXEIDKMIEKLECMSLEE 186
+V ASGH+D F D L+ + + YRADH +IE E
Sbjct: 75 REVWVASGHVDVFHDPLVESLITHKRYRADH-----------------LIEAYEAKHGHP 117
Query: 185 IDKVVEDYKILSKXK-NTLSKSQRFNLMFETFVGP--KKKNXIYXRPETAQGQFVNXXXV 15
+ D + + ++ + FN+M +T++GP ++ Y RPETAQG FVN V
Sbjct: 118 PPNGLADIRDPETGEPGQWTQPREFNMMLKTYLGPIETEEGLHYLRPETAQGIFVNFANV 177
>gi|2147427|pir||S58522 glycyl-tRNA synthetase - Thermus thermophilus
Length = 506
Score = 78.4 bits (190), Expect = 4e-14
Identities = 59/186 (31%), Positives = 93/186 (49%), Gaps = 18/186 (9%)
Query: 566 PMNKAERRETMLRKRXFIASSFSIYGGVSGLYDLGPLGTSLKNQILRVF--RDIFCKDLF 393
P + + + ++R FI S IYGG+ G+YD GPLG LKN + + + R+++ +D
Sbjct: 2 PASSLDELVALCKRRGFIFQSSEIYGGLQGVYDYGPLGVELKNNLKQAWWRRNVYERDDM 61
Query: 392 E-IESTILTPYQVLKASGHIDKFCDILLFDSVNGECYRADH-------------YXXXXX 255
E +++++LT VL SGH F D ++ + + + YR DH Y
Sbjct: 62 EGLDASVLTHRLVLHYSGHEATFADPMVDNRITKKRYRLDHLLKEQPEEVLKRLYRAMEV 121
Query: 254 XXXXXXXEIDKMIEKLECMSLEEIDKVVEDYKILSKXKNTLSKSQRFNLMFETFVGPKKK 75
+ M++ E V D S + + FN+MF+ GP+
Sbjct: 122 EEENLHALVQAMMQAPERAGGAMTAAGVLD--PASGEPGDWTPPRYFNMMFQDLRGPRGG 179
Query: 74 NXI--YXRPETAQGQFVNXXXVYE 9
+ Y RPETAQG FVN V +
Sbjct: 180 RGLLAYLRPETAQGIFVNFKNVLD 203
>gi|2392169|pdb|1ATI|A Chain A, Crystal Structure Of Glycyl-Trna Synthetase From Thermus
Thermophilus >gi|2392170|pdb|1ATI|B Chain B, Crystal
Structure Of Glycyl-Trna Synthetase From Thermus
Thermophilus
Length = 505
Score = 76.9 bits (186), Expect = 1e-13
Identities = 58/176 (32%), Positives = 90/176 (50%), Gaps = 18/176 (10%)
Query: 536 MLRKRXFIASSFSIYGGVSGLYDLGPLGTSLKNQILRVF--RDIFCKDLFE-IESTILTP 366
+ ++R FI S IYGG+ G+YD GPLG LKN + + + R+++ +D E +++++LT
Sbjct: 11 LCKRRGFIFQSSEIYGGLQGVYDYGPLGVELKNNLKQAWWRRNVYERDDMEGLDASVLTH 70
Query: 365 YQVLKASGHIDKFCDILLFDSVNGECYRADH-------------YXXXXXXXXXXXXEID 225
VL SGH F D ++ + + + YR DH Y +
Sbjct: 71 RLVLHYSGHEATFADPMVDNRITKKRYRLDHLLKEQPEEVLKRLYRAMEVEEENLHALVQ 130
Query: 224 KMIEKLECMSLEEIDKVVEDYKILSKXKNTLSKSQRFNLMFETFVGPKKKNXI--YXRPE 51
M++ E V D S + + FN+MF+ GP+ + Y RPE
Sbjct: 131 AMMQAPERAGGAMTAAGVLD--PASGEPGDWTPPRYFNMMFQDLRGPRGGRGLLAYLRPE 188
Query: 50 TAQGQFVNXXXVYE 9
TAQG FVN V +
Sbjct: 189 TAQGIFVNFKNVLD 202
>gi|2500997|sp|P75425|SYG_MYCPN GLYCYL-TRNA SYNTHETASE (GLYCINE--TRNA LIGASE) (GLYRS)
>gi|2146142|pir||S73808 glycine--tRNA ligase (EC
6.1.1.14) grs1 - Mycoplasma pneumoniae (SGC3) (ATCC
29342) >gi|1674173 (AE000047) Mycoplasma pneumoniae,
glycyl-tRNA synthetase; similar to Swiss-Prot Accession
Number P38088, from S. cerevisiae [Mycoplasma
pneumoniae]
Length = 449
Score = 76.9 bits (186), Expect = 1e-13
Identities = 53/176 (30%), Positives = 88/176 (49%), Gaps = 5/176 (2%)
Query: 533 LRKRXFIASSFSIYGGVSGLYDLGPLGTSLKNQILRVFRDIFCK---DLFEIESTILTPY 363
L++ F+ S +Y G++ +D GPLG LK QI + F K D+ I++ I+
Sbjct: 14 LKRYGFVFQSSELYNGLANSWDFGPLGAVLKQQIKTALYNFFIKNKRDVLLIDTPIILNE 73
Query: 362 QVLKASGHIDKFCDILLFDSVNGECYRADHYXXXXXXXXXXXXEIDKMIEKLECMSLEEI 183
Q+ KASGH+ F D L+ +R DH +D+ I+ + +++
Sbjct: 74 QIWKASGHLANFTDALVDCKSCKLRFRVDH--------------LDEQIKSATQWNPKQV 119
Query: 182 DKVVEDYKILSKXKNTLSKSQRFNLMFETFVG--PKKKNXIYXRPETAQGQFVNXXXVYE 9
+ + N S+ + FNL+F+T +G +K +Y RPETAQG F+N + +
Sbjct: 120 N-------CPNCKANNWSEVRDFNLLFQTEIGVVNSEKRLVYLRPETAQGIFINFKQLLQ 172
Query: 8 L 6
L
Sbjct: 173 L 173
>gi|2829475|sp|P56206|SYG_THETH GLYCYL-TRNA SYNTHETASE (GLYCINE--TRNA LIGASE) (GLYRS)
Length = 506
Score = 76.9 bits (186), Expect = 1e-13
Identities = 58/176 (32%), Positives = 90/176 (50%), Gaps = 18/176 (10%)
Query: 536 MLRKRXFIASSFSIYGGVSGLYDLGPLGTSLKNQILRVF--RDIFCKDLFE-IESTILTP 366
+ ++R FI S IYGG+ G+YD GPLG LKN + + + R+++ +D E +++++LT
Sbjct: 12 LCKRRGFIFQSSEIYGGLQGVYDYGPLGVELKNNLKQAWWRRNVYERDDMEGLDASVLTH 71
Query: 365 YQVLKASGHIDKFCDILLFDSVNGECYRADH-------------YXXXXXXXXXXXXEID 225
VL SGH F D ++ + + + YR DH Y +
Sbjct: 72 RLVLHYSGHEATFADPMVDNRITKKRYRLDHLLKEQPEEVLKRLYRAMEVEEENLHALVQ 131
Query: 224 KMIEKLECMSLEEIDKVVEDYKILSKXKNTLSKSQRFNLMFETFVGPKKKNXI--YXRPE 51
M++ E V D S + + FN+MF+ GP+ + Y RPE
Sbjct: 132 AMMQAPERAGGAMTAAGVLD--PASGEPGDWTPPRYFNMMFQDLRGPRGGRGLLAYLRPE 189
Query: 50 TAQGQFVNXXXVYE 9
TAQG FVN V +
Sbjct: 190 TAQGIFVNFKNVLD 203
>gi|2688266 (AE001142) glycyl-tRNA synthetase (glyS) # [Borrelia burgdorferi]
Length = 445
Score = 71.0 bits (171), Expect = 7e-12
Identities = 51/177 (28%), Positives = 81/177 (44%), Gaps = 5/177 (2%)
Query: 539 TMLRKRXFIASSFSIYGGVSGLYDLGPLGTSLKNQILRVFRDIFC---KDLFEIESTILT 369
++ +++ F+ S +YGG+SG +D GPLG LK I + + +++ ++S I
Sbjct: 9 SLAKRKGFVFQSSEVYGGLSGAWDYGPLGVELKKNIKKEWWKSMVYLHENIVGLDSAIFM 68
Query: 368 PYQVLKASGHIDKFCDILLFDSVNGECYRADHYXXXXXXXXXXXXEIDKMIEKLECMSLE 189
++ +ASGH+D F D ++ +RAD ID C
Sbjct: 69 RPEIWRASGHVDGFSDSMVDCKDCKSRFRAD--------------FIDLSKNCPNC---- 110
Query: 188 EIDKVVEDYKILSKXKNTLSKSQRFNLMFETFVG--PKKKNXIYXRPETAQGQFVNXXXV 15
K N + + FNLMF+T +G + +Y RPETAQG FVN V
Sbjct: 111 -------------KVGNNFTSPRSFNLMFKTHIGVVEDSSSEVYLRPETAQGIFVNFRNV 157
Query: 14 YE 9
+
Sbjct: 158 LD 159
>gi|1351155|sp|P47493|SYG_MYCGE GLYCYL-TRNA SYNTHETASE (GLYCINE--TRNA LIGASE) (GLYRS)
>gi|1361523|pir||G64227 glycine--tRNA ligase (EC
6.1.1.14) - Mycoplasma genitalium (SGC3) >gi|3844849
(U39704) glycyl-tRNA synthetase [Mycoplasma genitalium]
Length = 446
Score = 71.0 bits (171), Expect = 7e-12
Identities = 50/175 (28%), Positives = 79/175 (44%), Gaps = 5/175 (2%)
Query: 533 LRKRXFIASSFSIYGGVSGLYDLGPLGTSLKNQILRVFRDIFCK---DLFEIESTILTPY 363
L++ F+ S IY G++ +D GPLG LK QI + + F K D+ +E+ I+
Sbjct: 14 LKQHGFVFQSSEIYNGLNNSWDFGPLGAVLKQQIKQALYNFFIKNKADVLLVETPIILSE 73
Query: 362 QVLKASGHIDKFCDILLFDSVNGECYRADHYXXXXXXXXXXXXEIDKMIEKLECMSLEEI 183
V KASGH+ F D L+ +C + + D K+ C +
Sbjct: 74 LVWKASGHLANFVDTLV------DCKSCKYRFRVDQINAEIKAKKDWNSFKVNCPNCH-- 125
Query: 182 DKVVEDYKILSKXKNTLSKSQRFNLMFETFVG--PKKKNXIYXRPETAQGQFVNXXXVYE 9
S+ + FNL+F+T +G K ++ RPETAQG F+N + +
Sbjct: 126 -------------NQNWSEVRDFNLLFQTEIGVVNNDKRLVFLRPETAQGSFINFKNILQ 172
>gi|3322974 (AE001241) glycyl-tRNA synthetase (glyS) [Treponema pallidum]
Length = 462
Score = 70.2 bits (169), Expect = 1e-11
Identities = 50/176 (28%), Positives = 79/176 (44%), Gaps = 5/176 (2%)
Query: 536 MLRKRXFIASSFSIYGGVSGLYDLGPLGTSLKNQILRVFRDIFCK---DLFEIESTILTP 366
+ ++R F+ S IYGG G++D GP+G +LKN I + + + +++ IL
Sbjct: 7 LCKRRGFVFPSSEIYGGQGGVWDYGPMGIALKNNIAHAWWQDMTRLHDHIVGLDAAILMH 66
Query: 365 YQVLKASGHIDKFCDILLFDSVNGECYRADHYXXXXXXXXXXXXEIDKMIEKLECMSLEE 186
V + SGH+D F D L+ +V +RAD
Sbjct: 67 PNVWRTSGHVDHFSDPLVDCTVCKSRFRADQVAVPSAGGP-------------------- 106
Query: 185 IDKVVEDYKILSKXKNTLSKSQRFNLMFETFVGP--KKKNXIYXRPETAQGQFVNXXXVY 12
+ L+ + FNLMF T +GP ++ + +Y RPETAQG +VN V
Sbjct: 107 ----------CPQCGGALTGVRNFNLMFSTHMGPTDERASLLYLRPETAQGIYVNYKNVL 156
Query: 11 E 9
+
Sbjct: 157 Q 157
>gi|2664208|emb|CAA10903| (AJ222643) Glycyl-tRNA synthetase [Thermus thermophilus]
Length = 506
Score = 68.3 bits (164), Expect = 5e-11
Identities = 52/175 (29%), Positives = 88/175 (49%), Gaps = 16/175 (9%)
Query: 566 PMNKAERRETMLRKRXFIASSFSIYGGVSGLYDLGPLGTSLKNQILRVF--RDIFCKDLF 393
P + + + ++R FI S IYGG+ G+YD GPLG LKN + + + R+++ +D
Sbjct: 2 PASSLDELVALCKRRGFIFQSSEIYGGLQGVYDYGPLGVELKNNLKQAWWRRNVYERDDM 61
Query: 392 E-IESTILTPYQVLKASGHIDKFCDILLFDSVNGECYRADHYXXXXXXXXXXXXEIDKMI 216
E +++++LT VL SGH F D ++ + + + YR DH +
Sbjct: 62 EGLDASVLTHRLVLHYSGHEATFADPMVDNRITKKRYRLDHLLKEQPEEVLKRLTGPWRV 121
Query: 215 EKLECMSL--------EEIDKVVEDYKIL---SKXKNTLSKSQRFNLMFETFVGPKKKNX 69
E+ +L E + +L S + + FN+MF+ GP+
Sbjct: 122 EEENLHALVQAMMQAPERAGGAMTAAGVLDPASGEPGDWTPPRYFNMMFQDLRGPRGGRG 181
Query: 68 I--YXRPETAQ 42
+ Y RPETA+
Sbjct: 182 LLAYLRPETAR 192
>gi|4388929|pdb|1B76|A Chain A, Glycyl-Trna Synthetase From Thermus Thermophilus Complexed
With Atp >gi|4388930|pdb|1B76|B Chain B, Glycyl-Trna
Synthetase From Thermus Thermophilus Complexed With Atp
>gi|4388931|pdb|1GGM|A Chain A, Glycyl-Trna Synthetase
From Thermus Thermophilus Complexed With
Glycyl-Adenylate >gi|4388932|pdb|1GGM|B Chain B,
Glycyl-Trna Synthetase From Thermus Thermophilus
Complexed With Glycyl-Adenylate
Length = 442
Score = 63.6 bits (152), Expect = 1e-09
Identities = 50/176 (28%), Positives = 76/176 (42%), Gaps = 5/176 (2%)
Query: 536 MLRKRXFIASSFSIYGGVSGLYDLGPLGTSLKNQILRVF--RDIFCKDLFE-IESTILTP 366
+ ++R FI S IYGG+ G+YD GPLG LKN + + + R+++ +D E +++++LT
Sbjct: 11 LCKRRGFIFQSSEIYGGLQGVYDYGPLGVELKNNLKQAWWRRNVYERDDMEGLDASVLTH 70
Query: 365 YQVLKASGHIDKFCDILLFDSVNGECYRADHYXXXXXXXXXXXXEIDKMIEKLECMSLEE 186
VL SGH F D ++ D+ + Y
Sbjct: 71 RLVLHYSGHEATFADPMV-DNAKARYWTPPRY---------------------------- 101
Query: 185 IDKVVEDYKILSKXKNTLSKSQRFNLMFETFVGPKKKNXI--YXRPETAQGQFVNXXXVY 12
FN+MF+ GP+ + Y RPETAQG FVN V
Sbjct: 102 -----------------------FNMMFQDLRGPRGGRGLLAYLRPETAQGIFVNFKNVL 138
Query: 11 E 9
+
Sbjct: 139 D 139
>gi|2392770 (AC002534) similar to glycyl tRNA synthetase [Arabidopsis thaliana]
Length = 291
Score = 40.2 bits (92), Expect = 0.014
Identities = 17/46 (36%), Positives = 34/46 (72%), Gaps = 2/46 (4%)
Query: 449 SLKNQILRVFRDIFC--KDLFEIESTILTPYQVLKASGHIDKFCDILL 312
++K+ +L +R F ++++E++ +TP VLKASGH+D+F D+++
Sbjct: 39 AVKSNVLSFWRQHFILKENMYEVDCPCVTPEVVLKASGHVDQFTDLMV 86
>gi|1169007|sp|Q03727|COMA_STRPN TRANSPORT ATP-BINDING PROTEIN COMA >gi|520738 (M36180) comA protein
[Streptococcus pneumoniae]
Length = 717
Score = 30.9 bits (68), Expect = 9.0
Identities = 15/45 (33%), Positives = 25/45 (55%)
Query: 176 VVEDYKILSKXKNTLSKSQRFNLMFETFVGPKKKNXIYXRPETAQ 42
++ED + K+ S+SQR+ + + FV KK+ Y R E+ Q
Sbjct: 347 IIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQ 391
>gi|98020|pir||A39203 comA protein - Streptococcus pneumoniae
Length = 717
Score = 30.9 bits (68), Expect = 9.0
Identities = 15/45 (33%), Positives = 25/45 (55%)
Query: 176 VVEDYKILSKXKNTLSKSQRFNLMFETFVGPKKKNXIYXRPETAQ 42
++ED + K+ S+SQR+ + + FV KK+ Y R E+ Q
Sbjct: 347 IIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQ 391
Database: Non-redundant GenBank CDS
translations+PDB+SwissProt+SPupdate+PIR
Posted date: Apr 17, 1999 5:33 AM
Number of letters in database: 113,023,754
Number of sequences in database: 369,800
Lambda K H
0.318 0.135 0.00
Gapped
Lambda K H
0.270 0.0470 4.94e-324
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 95588488
Number of Sequences: 369800
Number of extensions: 1580248
Number of successful extensions: 3284
Number of sequences better than 10.0: 56
Number of HSP's better than 10.0 without gapping: 28
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 3203
Number of HSP's gapped (non-prelim): 38
length of query: 226
length of database: 113023754
effective HSP length: 53
effective length of query: 173
effective length of database: 93424354
effective search space: 16162413242
effective search space used: 16162413242
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 68 (30.9 bits)