BLASTX 2.0.8 [Jan-05-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 14VII34R
(713 letters)
Database: Non-redundant GenBank CDS
translations+PDB+SwissProt+SPupdate+PIR
369,800 sequences; 113,023,754 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gi|2264369 (AC002354) predicted protein of unknown function [Ara... 37 2e-04
gi|2558654 (AC002354) No definition line found [Arabidopsis thal... 37 3e-04
gi|4007798|emb|CAA22438| (AL034463) tRNA-splicing endonuclease p... 36 0.37
gi|3650382|emb|CAA21091| (AL031740) putative calcium dependant s... 35 0.64
gi|3702625|emb|CAA21218| (AL031824) hypothetical protein [Schizo... 35 0.64
gi|1255891 (U53344) similar to the type-B carboxylesterase/lipas... 34 1.4
gi|2688051 (AE001127) antigen, S2, putative [Borrelia burgdorferi] 32 4.2
gi|80059|pir||JS0029 adenylate cyclase (EC 4.6.1.1) precursor, c... 32 4.2
gi|729244|sp|P40136|CYAA_BACAN CALMODULIN-SENSITIVE ADENYLATE CY... 32 4.2
gi|2501102|sp|Q42374|SYNK_ARATH SYNTAXIN-RELATED PROTEIN KNOLLE ... 32 5.6
>gi|2264369 (AC002354) predicted protein of unknown function [Arabidopsis
thaliana]
Length = 722
Score = 36.7 bits (83), Expect = 2e-04
Identities = 30/111 (27%), Positives = 56/111 (50%), Gaps = 6/111 (5%)
Query: 596 SISYLYFIIRPFDIGDKIEVSKNEYTVYEMGLLYSTLI-CDSKFHNISNEILRKEPITNL 420
SI +L FII P+D+GD++ + E V EM +L + + D+ N +L ++ I N
Sbjct: 535 SIIFL-FIIHPYDVGDRLLIDTVEMVVEEMNILTTVFLRADNLKIVYPNILLWQKAIHNY 593
Query: 419 RRSKYITEEFKNKFSYDSVIGKLQKLEDEIDKFLASHXXIYNXKS-----EIRDINI 264
RS + +E + K+ ++ I ++ S + K+ ++ D+NI
Sbjct: 594 NRSPDMGDEVTCCVHITTPPEKIAAIKQRISSYIDSKPEYWYPKADVIVKDVEDLNI 650
Score = 29.0 bits (63), Expect = 2e-04
Identities = 12/35 (34%), Positives = 22/35 (62%)
Query: 682 VFTAGLNMVTTGIIFPLSFIFGNMLSDIFQSLIFI 578
+ T+ + T + L+F+FGN L +F+S+IF+
Sbjct: 505 IATSKYLLFLTSQVVLLAFMFGNSLKTVFESIIFL 539
>gi|2558654 (AC002354) No definition line found [Arabidopsis thaliana]
Length = 1569
Score = 36.7 bits (83), Expect = 3e-04
Identities = 23/93 (24%), Positives = 49/93 (51%), Gaps = 4/93 (4%)
Query: 596 SISYLYFIIRPFDIGDKIEVSKNEYTVYEMGLLYSTLICDSKFHNIS----NEILRKEPI 429
SI +L FI+ P+D+GD+ E+ + V EM +L + + ++ N+ N +L ++ I
Sbjct: 1383 SIIFL-FIVHPYDVGDRCEIDSVQLVVEEMNILTTVFL---RYDNLKIMYPNSLLWQKSI 1438
Query: 428 TNLRRSKYITEEFKNKFSYDSVIGKLQKLEDEIDKFL 318
N RS + + + + + K+ ++ I ++
Sbjct: 1439 NNYYRSPDMGDAIEFCVHITTPLEKISVIKQRISNYI 1475
Score = 28.2 bits (61), Expect = 3e-04
Identities = 10/19 (52%), Positives = 16/19 (83%)
Query: 634 LSFIFGNMLSDIFQSLIFI 578
L+FIFGN + +F+S+IF+
Sbjct: 1369 LAFIFGNTVKTVFESIIFL 1387
>gi|4007798|emb|CAA22438| (AL034463) tRNA-splicing endonuclease positive effector
[Schizosaccharomyces pombe]
Length = 1944
Score = 35.6 bits (80), Expect = 0.37
Identities = 24/105 (22%), Positives = 48/105 (44%)
Query: 554 GDKIEVSKNEYTVYEMGLLYSTLICDSKFHNISNEILRKEPITNLRRSKYITEEFKNKFS 375
GD I V E+T+ E +++++ +TNL++ + +
Sbjct: 1373 GDSISVHAKEFTLEE-------------------QMIKQMELTNLKKDQEANNSSDTRKK 1413
Query: 374 YDSVIGKLQKLEDEIDKFLASHXXIYNXKSEIRDINITDQGTALE 240
YDS+I K L ++++KF ++ ++++R+ IT Q LE
Sbjct: 1414 YDSIIKKRDSLREDLEKFRSTGKNSSILEAQLRE--ITKQKNMLE 1456
>gi|3650382|emb|CAA21091| (AL031740) putative calcium dependant serine-threonine protein
kinase. [Schizosaccharomyces pombe]
Length = 1011
Score = 34.8 bits (78), Expect = 0.64
Identities = 27/114 (23%), Positives = 54/114 (46%), Gaps = 7/114 (6%)
Query: 611 ALRYISISYLYFIIRPFDIGDKIEVSKN----EYTVYEMGLLYSTL-ICDSKFHNISNEI 447
A +S F+ P+D+GD+++V N V E+ ++ + + K N +
Sbjct: 642 AQELLSSIIFVFVKHPYDVGDRVDVMINGTVTSAMVKEISIMSTEFRLLTGKVIQAPNSL 701
Query: 446 LRKEPITNLRRSKYITEEFKNKFSYDSVIGKLQKLEDEIDKFLASHXXIY--NXKSEIRD 273
L I N+RRS I + + + + ++++L +I FL Y + +E+ D
Sbjct: 702 LNTLWILNMRRSDGIADPVTVNLKFGTTLQQIEQLRIKIIDFLKEEKRDYKPDLLTEVTD 761
Query: 272 I 270
+
Sbjct: 762 L 762
>gi|3702625|emb|CAA21218| (AL031824) hypothetical protein [Schizosaccharomyces pombe]
Length = 977
Score = 34.8 bits (78), Expect = 0.64
Identities = 27/114 (23%), Positives = 54/114 (46%), Gaps = 7/114 (6%)
Query: 611 ALRYISISYLYFIIRPFDIGDKIEVSKN----EYTVYEMGLLYSTL-ICDSKFHNISNEI 447
A +S F+ P+D+GD+++V N V E+ ++ + + K N +
Sbjct: 608 AQELLSSIIFVFVKHPYDVGDRVDVMINGTVTSAMVKEISIMSTEFRLLTGKVIQAPNSL 667
Query: 446 LRKEPITNLRRSKYITEEFKNKFSYDSVIGKLQKLEDEIDKFLASHXXIY--NXKSEIRD 273
L I N+RRS I + + + + ++++L +I FL Y + +E+ D
Sbjct: 668 LNTLWILNMRRSDGIADPVTVNLKFGTTLQQIEQLRIKIIDFLKEEKRDYKPDLLTEVTD 727
Query: 272 I 270
+
Sbjct: 728 L 728
>gi|1255891 (U53344) similar to the type-B carboxylesterase/lipase family
[Caenorhabditis elegans]
Length = 465
Score = 33.6 bits (75), Expect = 1.4
Identities = 18/53 (33%), Positives = 29/53 (53%), Gaps = 2/53 (3%)
Query: 485 ICDSKFHNISNEILRKEPITNLRRSKYIT--EEFKNKFSYDSVIGKLQKLEDEID 327
I D+ FH ++E L + + + + E N+F D V+G LQKLE +I+
Sbjct: 260 IIDNSFHETASEKLANSTVQLIANATIVRYLETPNNEFIADQVVGALQKLESDIE 314
>gi|2688051 (AE001127) antigen, S2, putative [Borrelia burgdorferi]
Length = 238
Score = 32.1 bits (71), Expect = 4.2
Identities = 30/83 (36%), Positives = 45/83 (54%), Gaps = 21/83 (25%)
Query: 485 ICDSKFHNISNEILRKE-PITNLR------RSKYIT---------EEFKNKFSYDSVIGK 354
I D K H IS +I K+ PI N ++K +T EFKNKF Y +I
Sbjct: 40 ISDEKLHLISGKISNKKLPIINSNHDVTWIKTKAMTILGEDGKEIPEFKNKFGYSYIISP 99
Query: 353 LQKLEDEIDKFLASHXXIYNXKS-----EIRDINITDQGTALEL 237
+ K++ + + + K+ EI D+ G+ LEL
Sbjct: 100 V-KMDGKYSYYASLLILFETTKNGDDEYEIEDVKFVTAGSTLEL 142
>gi|80059|pir||JS0029 adenylate cyclase (EC 4.6.1.1) precursor, calmodulin-sensitive -
Bacillus anthracis >gi|142813 (M24074) edema factor
[Bacillus anthracis]
Length = 800
Score = 32.1 bits (71), Expect = 4.2
Identities = 29/104 (27%), Positives = 46/104 (43%), Gaps = 15/104 (14%)
Query: 560 DIGDKIEVSKNEYTVYEMGLLYSTLICDSKFHNISNEILRKEP---------------IT 426
D GDK E +K E G L S ++N +N I +E I
Sbjct: 681 DSGDKDEFAKKESVKKIAGYL-------SDYYNSANHIFSQEKKRKISIFRGIQAYNEIE 733
Query: 425 NLRRSKYITEEFKNKFSYDSVIGKLQKLEDEIDKFLASHXXIYNXKSEIRDINITDQGT 249
N+ +SK I E+KN F Y +++ +++ L K + +N T+ T
Sbjct: 734 NVLKSKQIAPEYKNYFQY-----LKERITNQVQLLLTHQKSNIEFKLLYKQLNFTENET 787
>gi|729244|sp|P40136|CYAA_BACAN CALMODULIN-SENSITIVE ADENYLATE CYCLASE PRECURSOR (ATP
PYROPHOSPHATE-LYASE) (ADENYLYL CYCLASE) (EDEMA FACTOR)
(EF) >gi|142815 (M23179) edema factor [Bacillus
anthracis]
Length = 800
Score = 32.1 bits (71), Expect = 4.2
Identities = 29/104 (27%), Positives = 46/104 (43%), Gaps = 15/104 (14%)
Query: 560 DIGDKIEVSKNEYTVYEMGLLYSTLICDSKFHNISNEILRKEP---------------IT 426
D GDK E +K E G L S ++N +N I +E I
Sbjct: 681 DSGDKDEFAKKESVKKIAGYL-------SDYYNSANHIFSQEKKRKISIFRGIQAYNEIE 733
Query: 425 NLRRSKYITEEFKNKFSYDSVIGKLQKLEDEIDKFLASHXXIYNXKSEIRDINITDQGT 249
N+ +SK I E+KN F Y +++ +++ L K + +N T+ T
Sbjct: 734 NVLKSKQIAPEYKNYFQY-----LKERITNQVQLLLTHQKSNIEFKLLYKQLNFTENET 787
>gi|2501102|sp|Q42374|SYNK_ARATH SYNTAXIN-RELATED PROTEIN KNOLLE >gi|1184165 (U39451)
syntaxin-related [Arabidopsis thaliana] >gi|1184167
(U39452) syntaxin-related [Arabidopsis thaliana]
>gi|3063443 (AC003981) F22O13.4 [Arabidopsis thaliana]
>gi|1587182|prf||2206310A syntaxin-related protein
[Arabidopsis thaliana]
Length = 310
Score = 31.7 bits (70), Expect = 5.6
Identities = 28/114 (24%), Positives = 53/114 (45%), Gaps = 8/114 (7%)
Query: 527 EYTVYEMGLLYSTLICDSKFHNISNEILRKEPITNLRR--SKYITEEFKNKFSYDSVIGK 354
EY EMGL+ TL ++H S + + E + +LR S I + S S + +
Sbjct: 53 EYVKAEMGLISETLARIEQYHEESKGVHKAESVKSLRNKISNEIVSGLRKAKSIKSKLEE 112
Query: 353 LQKLEDEIDKF-----LASHXXIYN-XKSEIRDINITDQGTALELIISIKLLCPYQEVQE 192
+ K EI + S + N + +++++ + QG + K++ Y+E E
Sbjct: 113 MDKANKEIKRLSGTPVYRSRTAVTNGLRKKLKEVMMEFQG------LRQKMMSEYKETVE 166
Query: 191 AK 186
+
Sbjct: 167 RR 168
Database: Non-redundant GenBank CDS
translations+PDB+SwissProt+SPupdate+PIR
Posted date: Apr 17, 1999 5:33 AM
Number of letters in database: 113,023,754
Number of sequences in database: 369,800
Lambda K H
0.318 0.135 0.00
Gapped
Lambda K H
0.270 0.0470 4.94e-324
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 118388761
Number of Sequences: 369800
Number of extensions: 2167823
Number of successful extensions: 5401
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 5396
Number of HSP's gapped (non-prelim): 12
length of query: 237
length of database: 113023754
effective HSP length: 53
effective length of query: 184
effective length of database: 93424354
effective search space: 17190081136
effective search space used: 17190081136
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 68 (30.9 bits)