BLASTX 2.0.8 [Jan-05-1999] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 14VII33R (532 letters) Database: Non-redundant GenBank CDS translations+PDB+SwissProt+SPupdate+PIR 368,476 sequences; 112,640,273 total letters Searching...................................................done Score E Sequences producing significant alignments: (bits) Value gi|3845107 (AE001375) hypothetical protein [Plasmodium falciparum] 34 0.97 gi|2649562 (AE001032) purine NTPase, putative [Archaeoglobus ful... 32 2.2 gi|4160656 (AF103869) blood-stage membrane protein Ag-1 [Plasmod... 32 2.2 gi|2120485|pir||S61468 p83/100 protein - Lyme disease spirochete... 32 2.9 gi|1469496 (U49743) apical membrane antigen 1 [Plasmodium chabaudi] 32 2.9 gi|3845142 (AE001384) hypothetical protein [Plasmodium falciparum] 32 3.7 gi|3257342|dbj|BAA30025| (AP000004) 879aa long hypothetical puri... 31 4.9 gi|2120484|pir||S61467 p83/100 protein - Lyme disease spirochete... 31 6.4 gi|94578|pir||A60332 80K antigen - Lyme disease spirochete 31 6.4 gi|2688679 (AE001174) antigen, p83/100 [Borrelia burgdorferi] 31 6.4 gi|4103336 (AF022647) DNA relaxation protein homolog [Fusobacter... 31 6.4 gi|2120483|pir||S61465 p83/100 protein - Lyme disease spirochete... 31 6.4 gi|113684|sp|P16445|AMA1_PLACH APICAL MEMBRANE ANTIGEN 1 PRECURS... 31 6.4 gi|2120486|pir||S61466 p83/100 protein - Lyme disease spirochete... 31 8.4 gi|1491638|emb|CAA68190| (X99922) male sterility protein 2 [Bras... 31 8.4 gi|100519|pir||S22041 hypothetical protein 873 - common sunflowe... 31 8.4
>gi|3845107 (AE001375) hypothetical protein [Plasmodium falciparum] Length = 1979 Score = 33.6 bits (75), Expect = 0.97 Identities = 20/60 (33%), Positives = 38/60 (63%), Gaps = 5/60 (8%) Query: 300 NEDEGKLEEML---GSLKNDSLNFD--IKEAVKNIEKMKDETFEEEIYKGKISFMVFFVK 136 NE E +L++ L SL N +N++ I+E K ++++KD+ + Y+ K+ FVK Sbjct: 128 NELENQLKDTLKSISSLSNKIVNYESKIEELEKELKEVKDKNIDNNDYENKLKEKEDFVK 187 Query: 135 ELIEI 121 + I++ Sbjct: 188 QKIDM 192
>gi|2649562 (AE001032) purine NTPase, putative [Archaeoglobus fulgidus] Length = 886 Score = 32.5 bits (72), Expect = 2.2 Identities = 25/89 (28%), Positives = 42/89 (47%), Gaps = 1/89 (1%) Query: 336 LLKHKGRRFNVKNEDEGKLEEMLG-SLKNDSLNFDIKEAVKNIEKMKDETFEEEIYKGKI 160 L +HK R +++ ++ L+E+ G K L +KE V+ IE ++ + E + K K Sbjct: 227 LEEHKSRLESLRKQESSVLQEVRGLEEKLRELEKQLKEVVERIEDLEKKAKEVKELKPKA 286 Query: 159 SFMVFFVKELIEINGNMKIYVSTDEKLTEE 70 K L EIN ++ + LT E Sbjct: 287 ERYSILEKLLSEINQALRDVEKREGDLTRE 316
>gi|4160656 (AF103869) blood-stage membrane protein Ag-1 [Plasmodium yoelii] Length = 549 Score = 32.5 bits (72), Expect = 2.2 Identities = 19/44 (43%), Positives = 26/44 (58%), Gaps = 2/44 (4%) Query: 306 VKNEDEGKLEEMLGSLKNDSLNFD--IKEAVKNIEKMKDETFEEEI 175 VKN++E K EE +KND D + VKN E+ KDE +E+ Sbjct: 368 VKNDEEKKDEEKKDEVKNDEEKKDEVKNDEVKNDEEKKDEVKNDEV 413 Score = 32.1 bits (71), Expect = 2.9 Identities = 19/57 (33%), Positives = 31/57 (54%) Query: 330 KHKGRRFNVKNEDEGKLEEMLGSLKNDSLNFDIKEAVKNIEKMKDETFEEEIYKGKI 160 K + ++ VKN++E K E +KND + K+ VKN E DE +E+ K ++ Sbjct: 375 KDEEKKDEVKNDEEKKDEVKNDEVKNDE---EKKDEVKNDEVKTDEVKNDEVKKDEV 428 Score = 31.3 bits (69), Expect = 4.9 Identities = 19/42 (45%), Positives = 28/42 (66%), Gaps = 2/42 (4%) Query: 300 NEDEGKLEEMLGSLKNDSL-NFDIK-EAVKNIEKMKDETFEEEI 175 N DE K EE +KND + N ++K + VKN E+ KDE ++E+ Sbjct: 340 NADEKKDEEKNDEVKNDEVKNDEVKNDEVKNDEEKKDEEKKDEV 383
>gi|2120485|pir||S61468 p83/100 protein - Lyme disease spirochete (strain pacificus) >gi|928982|emb|CAA57240| (X81520) p83/100 [Borrelia burgdorferi] Length = 89 Score = 32.1 bits (71), Expect = 2.9 Identities = 24/82 (29%), Positives = 39/82 (47%) Query: 318 RRFNVKNEDEGKLEEMLGSLKNDSLNFDIKEAVKNIEKMKDETFEEEIYKGKISFMVFFV 139 ++ +K DE ++L S +L+ D + K K K + EEEI KGK Sbjct: 2 KQIEIKKSDE----KLLKSKDGKALDLDRELNSKASSKEKSKAKEEEITKGKSQ------ 51 Query: 138 KELIEINGNMKIYVSTDEKLTE 73 K L ++N + + + D+KL E Sbjct: 52 KSLGDLNNDENLMMPEDQKLPE 73
>gi|1469496 (U49743) apical membrane antigen 1 [Plasmodium chabaudi] Length = 558 Score = 32.1 bits (71), Expect = 2.9 Identities = 25/84 (29%), Positives = 47/84 (55%), Gaps = 9/84 (10%) Query: 333 LKHKGRRFNVKNEDEGKLEEMLGSLKNDSL----NFDIKEAVKNIEKMKDETFEEEIYKG 166 +K KG+ +N N D+ + + + + K L NF A+ ++E+ E+F +IYK Sbjct: 335 VKSKGKGYNWANYDKKEKKCYIFNKKPTCLINDKNFVATTALSSLEEAPQESFPCDIYKK 394 Query: 165 KISFMVFFVKELIEINGN-----MKIYVSTDEK 82 KI+ + + NGN +I++S D++ Sbjct: 395 KIAEEIKVMNVNRNNNGNDTIKFPRIFISDDKE 427
>gi|3845142 (AE001384) hypothetical protein [Plasmodium falciparum] Length = 2539 Score = 31.7 bits (70), Expect = 3.7 Identities = 24/88 (27%), Positives = 43/88 (48%), Gaps = 2/88 (2%) Query: 264 SLKNDSLNFDIKEAVKNIEKMKDETFEEEIYKGKISFMVFFVKELIEINGNMKIYVSTDE 85 SLK + N ++K NI K+K ++++IY +++ ++ + G +KIY+ Sbjct: 1003 SLKLNINNLNVKYKGNNIYKIKTVMYQKDIYN-------YYLLNILLLVG-VKIYIRQHN 1054 Query: 84 KLTE--ELNVPFPGYYGFNAKENAYYRKEF 1 KL + E NV G +K + Y F Sbjct: 1055 KLNKESEYNVNSQNLIGSKSKSSKIYMVHF 1084
>gi|3257342|dbj|BAA30025| (AP000004) 879aa long hypothetical purine NTPase [Pyrococcus horikoshii] Length = 879 Score = 31.3 bits (69), Expect = 4.9 Identities = 21/73 (28%), Positives = 40/73 (54%), Gaps = 7/73 (9%) Query: 318 RRFNVKNEDEGKLEEMLGSLKNDSLNFDIKEAVKN-------IEKMKDETFEEEIYKGKI 160 R+F+ K +E + + M S++ L ++E + IEK+K+E E E K ++ Sbjct: 654 RKFDQKKYEEKREKMMKLSMEIKGLETKLEELERRRDEIKSTIEKLKEERKERESAKMEL 713 Query: 159 SFMVFFVKELIEINGNMKIY 100 + +K + E+ G +K Y Sbjct: 714 EKLNIAIKRIEELRGKIKEY 733
>gi|2120484|pir||S61467 p83/100 protein - Lyme disease spirochete (strain 297) >gi|928980|emb|CAA57234| (X81514) p83/100 [Borrelia burgdorferi] Length = 95 Score = 30.9 bits (68), Expect = 6.4 Identities = 23/82 (28%), Positives = 36/82 (43%), Gaps = 2/82 (2%) Query: 318 RRFNVKNEDEGKLEEMLGSLKNDSLNFDIKEAV--KNIEKMKDETFEEEIYKGKISFMVF 145 ++ +K DE L+ D D+ + K K K + EEEI KGK Sbjct: 2 KQIEIKKSDEKLLKSKDDKASKDGKALDLDRELNSKASSKEKSKAKEEEITKGKSQ---- 57 Query: 144 FVKELIEINGNMKIYVSTDEKLTE 73 K L ++N + + + D+KL E Sbjct: 58 --KSLGDLNNDENLMMPEDQKLPE 79
>gi|94578|pir||A60332 80K antigen - Lyme disease spirochete Length = 700 Score = 30.9 bits (68), Expect = 6.4 Identities = 23/82 (28%), Positives = 36/82 (43%), Gaps = 2/82 (2%) Query: 318 RRFNVKNEDEGKLEEMLGSLKNDSLNFDIKEAV--KNIEKMKDETFEEEIYKGKISFMVF 145 ++ +K DE L+ D D+ + K K K + EEEI KGK Sbjct: 382 KQIEIKKSDEKLLKSKDDKASKDGKALDLDRELNSKASSKEKSKAKEEEITKGKSQ---- 437 Query: 144 FVKELIEINGNMKIYVSTDEKLTE 73 K L ++N + + + D+KL E Sbjct: 438 --KSLGDLNNDENLMMPEDQKLPE 459
>gi|2688679 (AE001174) antigen, p83/100 [Borrelia burgdorferi] Length = 700 Score = 30.9 bits (68), Expect = 6.4 Identities = 23/82 (28%), Positives = 36/82 (43%), Gaps = 2/82 (2%) Query: 318 RRFNVKNEDEGKLEEMLGSLKNDSLNFDIKEAV--KNIEKMKDETFEEEIYKGKISFMVF 145 ++ +K DE L+ D D+ + K K K + EEEI KGK Sbjct: 382 KQIEIKKSDEKLLKSKDDKASKDGKALDLDRELNSKASSKEKSKAKEEEITKGKSQ---- 437 Query: 144 FVKELIEINGNMKIYVSTDEKLTE 73 K L ++N + + + D+KL E Sbjct: 438 --KSLGDLNNDENLMMPEDQKLPE 459
>gi|4103336 (AF022647) DNA relaxation protein homolog [Fusobacterium nucleatum] Length = 348 Score = 30.9 bits (68), Expect = 6.4 Identities = 17/59 (28%), Positives = 32/59 (53%), Gaps = 2/59 (3%) Query: 246 LNFDIKEAVKNIEKMK--DETFEEEIYKGKISFMVFFVKELIEINGNMKIYVSTDEKLTE 73 + F K+ K ++ ++TF++E +K + F + + E GN KI V+T+ + T Sbjct: 243 ITFKFKDEKKKSIRLANLEKTFQDETFKKEYLEQQFLINQKTEEIGNFKIKVNTEAEKTN 302 Query: 72 E 70 E Sbjct: 303 E 303
>gi|2120483|pir||S61465 p83/100 protein - Lyme disease spirochete (fragment) Length = 90 Score = 30.9 bits (68), Expect = 6.4 Identities = 23/82 (28%), Positives = 36/82 (43%), Gaps = 2/82 (2%) Query: 318 RRFNVKNEDEGKLEEMLGSLKNDSLNFDIKEAV--KNIEKMKDETFEEEIYKGKISFMVF 145 ++ +K DE L+ D D+ + K K K + EEEI KGK Sbjct: 2 KQIEIKKSDEKLLKSKDDKASKDGKALDLDRELNSKASSKEKSKAKEEEITKGKSQ---- 57 Query: 144 FVKELIEINGNMKIYVSTDEKLTE 73 K L ++N + + + D+KL E Sbjct: 58 --KSLGDLNNDENLMMPEDQKLPE 79
>gi|113684|sp|P16445|AMA1_PLACH APICAL MEMBRANE ANTIGEN 1 PRECURSOR (MEROZOITE SURFACE ANTIGEN) >gi|320934|pir||A44964 apical membrane antigen 1 - Plasmodium chabaudi adami >gi|160077 (M25248) apical membrane antigen I [Plasmodium chabaudi] Length = 558 Score = 30.9 bits (68), Expect = 6.4 Identities = 28/92 (30%), Positives = 49/92 (52%), Gaps = 9/92 (9%) Query: 333 LKHKGRRFNVKNEDEGKLEEMLGSLKNDSL----NFDIKEAVKNIEKMKDETFEEEIYKG 166 +K KG+ +N N D+ + + + K L +F A+ ++E+ E+F +IYK Sbjct: 335 VKSKGKGYNWANYDKKTKKCYIFNKKPTCLINDKDFVATTALSSLEEGPQESFPCDIYKK 394 Query: 165 KISFMVFFVKELIEINGN-----MKIYVSTDEKLTEELNVP 58 KI+ + + NGN +I++S D+ E LN P Sbjct: 395 KIAEEIKVMNVNRNNNGNDTIKFPRIFISDDK---ESLNCP 432
>gi|2120486|pir||S61466 p83/100 protein - Lyme disease spirochete (strain T255) >gi|928985|emb|CAA57250| (X81531) p83/100 [Borrelia burgdorferi] Length = 95 Score = 30.5 bits (67), Expect = 8.4 Identities = 23/82 (28%), Positives = 35/82 (42%), Gaps = 2/82 (2%) Query: 318 RRFNVKNEDEGKLEEMLGSLKNDSLNFDIKEAV--KNIEKMKDETFEEEIYKGKISFMVF 145 ++ +K DE L+ D D+ + K K K + EEEI KGK Sbjct: 2 KQIEIKKSDEKLLKSKDDKASKDGKALDLDRELNSKASSKEKSKAKEEEITKGKSQ---- 57 Query: 144 FVKELIEINGNMKIYVSTDEKLTE 73 K L ++N + + D+KL E Sbjct: 58 --KSLGDLNNDKNFMMPEDQKLPE 79
>gi|1491638|emb|CAA68190| (X99922) male sterility protein 2 [Brassica napus] Length = 616 Score = 30.5 bits (67), Expect = 8.4 Identities = 21/57 (36%), Positives = 39/57 (67%), Gaps = 10/57 (17%) Query: 231 KEAVKNIEKMKDETFEEEIYKGK-----ISFMVFFVKELIEINGNM---KIYVSTD--EK 82 KEA I+++K+E + E++K SFM F + +L+ + GN+ I + TD E+ Sbjct: 169 KEAA--IQRLKNEVLDAELFKNLRETHGASFMSFMLDKLVPVTGNICDSNIGLQTDSAEE 226 Query: 81 LTEELNV 61 + +E++V Sbjct: 227 IAKEVDV 233
>gi|100519|pir||S22041 hypothetical protein 873 - common sunflower >gi|12992|emb|CAA44478| (X62592) ORF 873 [Helianthus annuus] >gi|758363|emb|CAA37614| (X53537) orfH522 [Helianthus annuus] Length = 291 Score = 30.5 bits (67), Expect = 8.4 Identities = 19/71 (26%), Positives = 44/71 (61%), Gaps = 5/71 (7%) Query: 264 SLKNDSLNFDIKEAVKNIEKMKDETFEEEIYKGKISFMVFFVKELIEINGNMKIYVS--- 94 +++ ++ NF ++ +V+ + + + F++++ +G ++F F+KEL + N +IY + Sbjct: 221 NIRMEAYNF-LEASVEPVSAL-EHPFQKDLMEGTLNF---FIKELTQNGRNSEIYKNFYI 275 Query: 93 --TDEKLTEELNVPFP 52 TDE +L +P P Sbjct: 276 HFTDELFRVKLGLPLP 291 Database: Non-redundant GenBank CDS translations+PDB+SwissProt+SPupdate+PIR Posted date: Apr 12, 1999 12:54 PM Number of letters in database: 112,640,273 Number of sequences in database: 368,476 Lambda K H 0.318 0.135 0.00 Gapped Lambda K H 0.270 0.0470 4.94e-324 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 87820061 Number of Sequences: 368476 Number of extensions: 1562946 Number of successful extensions: 4360 Number of sequences better than 10.0: 32 Number of HSP's better than 10.0 without gapping: 4 Number of HSP's successfully gapped in prelim test: 17 Number of HSP's that attempted gapping in prelim test: 4350 Number of HSP's gapped (non-prelim): 24 length of query: 177 length of database: 112640273 effective HSP length: 52 effective length of query: 124 effective length of database: 93479521 effective search space: 11591460604 effective search space used: 11591460604 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.8 bits) X3: 64 (24.9 bits) S1: 41 (21.7 bits) S2: 66 (30.1 bits)