BLASTX 2.0.8 [Jan-05-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 14VII25R
(526 letters)
Database: Non-redundant GenBank CDS
translations+PDB+SwissProt+SPupdate+PIR
369,800 sequences; 113,023,754 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gi|84040|pir||E22845 hypothetical protein 4 - Trypanosoma brucei... 37 0.084
gi|83996|pir||B26696 hypothetical protein 1 (CYb-COII intergenic... 37 0.084
gi|2688154 (AE001136) cell division protein, putative [Borrelia ... 36 0.25
gi|1171822|sp|P24896|NU5M_CAEEL NADH-UBIQUINONE OXIDOREDUCTASE C... 32 2.2
gi|141028|sp|P04540|NU5M_TRYBB NADH-UBIQUINONE OXIDOREDUCTASE CH... 32 2.8
gi|418746|pir||S34960 NADH dehydrogenase (ubiquinone) (EC 1.6.5.... 32 2.8
gi|722345 (U22357) runt [Drosophila pseudoobscura] 32 3.7
gi|436923 (U01849) ORF1 [Trypanosoma brucei] 31 8.3
>gi|84040|pir||E22845 hypothetical protein 4 - Trypanosoma brucei mitochondrion (SGC6)
Length = 445
Score = 37.1 bits (84), Expect = 0.084
Identities = 32/116 (27%), Positives = 55/116 (46%), Gaps = 7/116 (6%)
Query: 36 ELQSLLKFVLFVQTYFXYSFL---NYYVDVXTSYV*XCSNFYKFVS*YLCIYFCSFYFLK 206
E+ SLL F+L + + F Y L YY+ +++ Y + Y C + C F FL
Sbjct: 109 EIFSLLLFLLLMSSRFGYKILVLWYYYMLNLINFILLFILLYFMILNY-CFFLCDFCFLV 167
Query: 207 *NRYWYNIFXRLFTK--LFKYQMTSWSRWIIKIFYR--YFVWLSDLTIFSVILFHYIIYX 374
+ W I +T LFK + ++ +++ R F+++ LT + + F II
Sbjct: 168 FDEEWLGILCLFYTLLILFKLYIAFLILFMEQLYIRLGVFIFIYMLTFYILFCFILIILL 227
Query: 375 XXF 383
F
Sbjct: 228 ISF 230
>gi|83996|pir||B26696 hypothetical protein 1 (CYb-COII intergenic region) -
Sauroleishmania tarentolae mitochondrion (SGC6)
(fragment) >gi|896286 (M10126) NH2 terminus uncertain
[Leishmania tarentolae]
Length = 443
Score = 37.1 bits (84), Expect = 0.084
Identities = 32/116 (27%), Positives = 54/116 (45%), Gaps = 7/116 (6%)
Query: 36 ELQSLLKFVLFVQTYFXYSFL---NYYVDVXTSYV*XCSNFYKFVS*YLCIYFCSFYFLK 206
EL SLL F++ + + F Y L YY+ +++ Y + Y C Y C F FL
Sbjct: 107 ELFSLLLFLILISSRFGYKILILWYYYMINLINFILLFVLLYYMILNY-CFYLCDFCFLI 165
Query: 207 *NRYWYNIFXRLFTK--LFKYQMTSWSRWIIKIFYR--YFVWLSDLTIFSVILFHYIIYX 374
+ W I + LFK + ++ +++ R F+++ LT + + F II
Sbjct: 166 FDEEWLGILCLFYILLILFKLYIAILILFLEQLYIRLGVFIFIYMLTFYVLFCFILIILL 225
Query: 375 XXF 383
F
Sbjct: 226 ICF 228
>gi|2688154 (AE001136) cell division protein, putative [Borrelia burgdorferi]
Length = 787
Score = 35.6 bits (80), Expect = 0.25
Identities = 31/113 (27%), Positives = 53/113 (46%), Gaps = 3/113 (2%)
Query: 183 FCSFYFLK*NRYWYNIFXRLFTKLFKYQMTSWSRWIIKIFYRYFVWLSDLTIFSVILFHY 362
F F F + N+F L L Y + +W + IF + F++ + ++VILF
Sbjct: 34 FVFFVFNLLGQILLNVFSFLSFYLIVYPIVNWYAYKKHIFTKRFIFNWN---YTVILFFT 90
Query: 363 IIYXXXFRR*NFMIFGKITYKIFQ---*ISYSNRFIYSIFVLGKFICWIFNNFAIF 521
+I+ N + KIF I N FI+++ +L +F+ WI+ N+ F
Sbjct: 91 LIFLIKI---NSNVEKSYFIKIFLINFGIVLGNFFIFTLLIL-EFVVWIYLNYVFF 142
>gi|1171822|sp|P24896|NU5M_CAEEL NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 5 >gi|102566|pir||S26037 NADH
dehydrogenase (ubiquinone) (EC 1.6.5.3) chain 5 -
Caenorhabditis elegans mitochondrion (SGC4)
>gi|515886|emb|CAA38162| (X54252) ND5 protein (AA 1 -
527) [Caenorhabditis elegans]
Length = 527
Score = 32.5 bits (72), Expect = 2.2
Identities = 23/86 (26%), Positives = 39/86 (44%)
Query: 48 LLKFVLFVQTYFXYSFLNYYVDVXTSYV*XCSNFYKFVS*YLCIYFCSFYFLK*NRYWYN 227
L F+LF+ T+ F YY++ ++ N+Y FV L I+ S + L N
Sbjct: 49 LFSFILFLVTFSVLVFSTYYLNSELNF-----NYYYFV---LLIFVGSMFSL-------N 93
Query: 228 IFXRLFTKLFKYQMTSWSRWIIKIFY 305
+FT L + + S + + +FY
Sbjct: 94 FSNSIFTMLLSWDLLGISSFFLVLFY 119
>gi|141028|sp|P04540|NU5M_TRYBB NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 5 >gi|76351|pir||QQUTC5 NADH
dehydrogenase (ubiquinone) (EC 1.6.5.3) chain 5 -
Trypanosoma brucei mitochondrion (SGC6) >gi|343540
(M14820) NADH dehydrogenase subunit 5 [Trypanosoma
brucei]
Length = 590
Score = 32.1 bits (71), Expect = 2.8
Identities = 44/158 (27%), Positives = 70/158 (43%), Gaps = 28/158 (17%)
Query: 51 LKFVLFVQTYFXYSFLN--------YYVD-----------VXTSYV*XCSNFYKFVS*YL 173
L ++F+ +SFL YY D + +YV CS FY F+ +
Sbjct: 32 LVMIIFIVLCMIFSFLMVSVCLYGYYYYDFCLILMLDFCFIWLTYV--CSGFYMFIMLLI 89
Query: 174 CIYFCSFYFLK*NRYWYNIFXRLFTKLFKYQMTSWSRWIIKI-FYRYFVWLSDLTIFSVI 350
+ FC F ++++ F +F + + +I+ F + L +FS
Sbjct: 90 NMVFCFIVFYAFYYMYFDMLLGRFLIIFWIFVVCMNLFILSYDFLTAYCGWELLGLFSFF 149
Query: 351 LFHYIIYXXXFRR*NFMIF--GKITYKI----FQ*ISYSNRFIYSIFVLGKFIC--WIFN 506
L Y Y + F F GKI + F I SN F + F F C + +
Sbjct: 150 LISYFWYRFFALKFGFKAFFIGKIGDVLLIFAFSIIFLSNGFCMTTFYFLNFFCMDYYYI 209
Query: 507 NFAIFL 524
F+I L
Sbjct: 210 EFSICL 215
>gi|418746|pir||S34960 NADH dehydrogenase (ubiquinone) (EC 1.6.5.3) chain 5 - Crithidia
oncopelti mitochondrion (SGC6) >gi|12882|emb|CAA39492|
(X56015) NADH dehydrogenase subunit 5 [Crithidia
oncopelti]
Length = 590
Score = 32.1 bits (71), Expect = 2.8
Identities = 31/129 (24%), Positives = 47/129 (36%)
Query: 138 CSNFYKFVS*YLCIYFCSFYFLK*NRYWYNIFXRLFTKLFKYQMTSWSRWIIKIFYRYFV 317
C Y F+ Y+C F + L+ +F WS ++ +Y F
Sbjct: 454 CCLIYCFLGLYMCFILIFF-----------VIDFLYIFVFSSYCMFWSFYL---YYFNFF 499
Query: 318 WLSDLTIFSVILFHYIIYXXXFRR*NFMIFGKITYKIFQ*ISYSNRFIYSIFVLGKFICW 497
++ T+F +I +I Y F F +F I I F+ G F CW
Sbjct: 500 DIASFTVFVMISLAFIYYGCVL----FYFFNVDCIMLFWRIFL---IICVTFMFGLFSCW 552
Query: 498 IFNNFAIFL 524
F F I+L
Sbjct: 553 YFACFFIYL 561
Score = 30.9 bits (68), Expect = 6.3
Identities = 24/105 (22%), Positives = 44/105 (41%), Gaps = 2/105 (1%)
Query: 57 FVLFVQTYFXYSFLNYYVDVXTSYV*XCSNFYKFVS*YLCIYFCSFYFLK*NRYWYNIFX 236
F L + + F+++Y C+ FY F+ + + FC F +Y++
Sbjct: 61 FCLILMLDLCFIFMSFY----------CNGFYLFILFLIDLVFCFILFYAFYYMYYDLML 110
Query: 237 RLFTKLFKYQMTSWSRWIIKIFY--RYFVWLSDLTIFSVILFHYIIY 371
+ F +F + + + +I+ Y Y W L +FS L Y Y
Sbjct: 111 KRFFNIFWWFVLCMNFFILSYDYLTAYCGW-ELLGLFSFFLISYFWY 156
>gi|722345 (U22357) runt [Drosophila pseudoobscura]
Length = 561
Score = 31.7 bits (70), Expect = 3.7
Identities = 14/52 (26%), Positives = 26/52 (49%)
Query: 470 NTINESITIGNLLENLISYFAKYHEILSSKXXXIYNIMEKYHGEYGKIGEPN 315
NT N + G+ N S AK ++ ++ ++++YHGE + G P+
Sbjct: 88 NTANNTANNGSSNSNSNSNSAKMPSSMTDMFASLHEMLQEYHGELAQTGSPS 139
>gi|436923 (U01849) ORF1 [Trypanosoma brucei]
Length = 146
Score = 30.5 bits (67), Expect = 8.3
Identities = 26/112 (23%), Positives = 44/112 (39%)
Query: 168 YLCIYFCSFYFLK*NRYWYNIFXRLFTKLFKYQMTSWSRWIIKIFYRYFVWLSDLTIFSV 347
YL Y C F+F+ + +F W+ +F F+W + F
Sbjct: 25 YLFCYSCLFFFVVLGCDFLLVF-----------------WLYSLF---FLWRYNFVYF-F 63
Query: 348 ILFHYIIYXXXFRR*NFMIFGKITYKIFQ*ISYSNRFIYSIFVLGKFICWIF 503
LF ++ + F F +FG Y + + + + F FVLG F ++F
Sbjct: 64 FLFCFVFFVLLFL---FGLFGFFLYFLLCFVLFFDLFFMLFFVLGGFFVFVF 112
Database: Non-redundant GenBank CDS
translations+PDB+SwissProt+SPupdate+PIR
Posted date: Apr 17, 1999 5:33 AM
Number of letters in database: 113,023,754
Number of sequences in database: 369,800
Lambda K H
0.318 0.135 0.00
Gapped
Lambda K H
0.270 0.0470 4.94e-324
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 85505392
Number of Sequences: 369800
Number of extensions: 1564283
Number of successful extensions: 3737
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 12
Number of HSP's that attempted gapping in prelim test: 3730
Number of HSP's gapped (non-prelim): 15
length of query: 175
length of database: 113023754
effective HSP length: 52
effective length of query: 122
effective length of database: 93794154
effective search space: 11442886788
effective search space used: 11442886788
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 66 (30.1 bits)