BLASTX 2.0.8 [Jan-05-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 14VII-35F
(704 letters)
Database: Non-redundant GenBank CDS
translations+PDB+SwissProt+SPupdate+PIR
368,476 sequences; 112,640,273 total letters
Searching...................................................done
Score E
Sequences producing significant alignments: (bits) Value
gi|1203939 (U49830) Similar to protein tyrosine phosphatase [Cae... 40 0.019
gi|3413|emb|CAA34421| (X16385) Baf1 protein (AA 1-731) [Saccharo... 38 0.095
gi|320637|pir||S29870 DNA-binding protein BAF1 - yeast (Saccharo... 38 0.095
gi|114814|sp|P14164|BAF1_YEAST TRANSCRIPTION FACTOR BAF1 (ARS BI... 38 0.095
gi|3914889|sp|O51453|RS13_BORBU 30S RIBOSOMAL PROTEIN S13 >gi|26... 34 0.82
gi|3861177|emb|CAA15077| (AJ235272) 30S RIBOSOMAL PROTEIN S13 (r... 32 3.2
gi|309820 (L20871) Antigen [Pneumocystis carinii] 32 3.2
gi|100901|pir||S19254 nitrate reductase (NADH) (EC 1.6.6.1) flav... 32 4.2
gi|3024085|sp|Q28824|KMLS_BOVIN MYOSIN LIGHT CHAIN KINASE, SMOOT... 32 5.4
gi|2961377|emb|CAA18124| (AL022141) putative receptor protein ki... 32 5.4
gi|902755 (U20460) hemagglutinin precursor [Influenza A virus] 31 7.1
gi|1944199|dbj|BAA19658| (AB002817) dihydroflavonol 4-reductase ... 31 9.4
>gi|1203939 (U49830) Similar to protein tyrosine phosphatase [Caenorhabditis
elegans]
Length = 815
Score = 39.9 bits (91), Expect = 0.019
Identities = 44/135 (32%), Positives = 63/135 (46%), Gaps = 17/135 (12%)
Query: 458 KNIPKKCLTAIVEENTEYDGLVKVYPTTTSPKLLSMMTYFDNS--AKPGFVTTKKKVGFK 285
K + C T + NT D +VY T P+ T DN+ +K G +KKK
Sbjct: 332 KRLDGTCATLTFDHNTPGDHFERVYVNITLPE----KTGQDNNQGSKKGQTDSKKKD--- 384
Query: 284 DISNSGYTYGKNKKEDGWQYVMSRQKS-------------IDEIKSSAKTLNP--WGRLK 150
S G +KK++ + R+ S I KS +P +GR K
Sbjct: 385 --QGSRKARGNSKKKNHSSKKVVRKTSKKKKNAASPNAQLIPVAKSPGNDKSPAGFGRKK 442
Query: 149 SSENPFETIKPLKTPSPSENSPLESANPFKTT 54
+ EN E +K L+ P+P LE N +KTT
Sbjct: 443 AGENLNEVMKHLRAPTPRAQQELEKTN-WKTT 473
>gi|3413|emb|CAA34421| (X16385) Baf1 protein (AA 1-731) [Saccharomyces cerevisiae]
Length = 731
Score = 37.5 bits (85), Expect = 0.095
Identities = 45/183 (24%), Positives = 77/183 (41%), Gaps = 22/183 (12%)
Query: 686 VGMENTRYESVCEKSKDVESDKIKNLKYYKLPVNDLDGEY-------------KEDASLA 546
+ N + S D NL + +D+ G + +++ SL
Sbjct: 290 INNNNVGSHGISSHSPSSIRDTSMNLDVFNSATDDIPGPFVVTKIEPYHSHPLEDNLSLG 349
Query: 545 SALKKDIPVSSGDSHKKDLPLSGGISYSGKNIPKKCLTAIVEENTEYDGLVKVYPTTTSP 366
+ IP + K D L + S + K ++ VEE+ D L++ Y T
Sbjct: 350 KFILTKIPKILQNDLKFDQILESSYNNSNHTVSKFKVSHYVEESGLLDILMQRYGLTAED 409
Query: 365 ---KLLSMMTYFDNSAKPGFVTTKKKVG-FKDISNSGYTYGKNKKEDGWQYVMSRQKSID 198
+LLS + + K FV KKK+G + D+ S + N + R SID
Sbjct: 410 FEKRLLSQIARRITTYKARFVLKKKKMGEYNDLQPSSSSNNNNNNDGELSGTNLRSNSID 469
Query: 197 -----EIKSSAKTLNPWGRLKSSEN 138
EI S+ + N + +++N
Sbjct: 470 YAKHQEISSAGTSSNTTKNVNNNKN 494
>gi|320637|pir||S29870 DNA-binding protein BAF1 - yeast (Saccharomyces cerevisiae)
>gi|486187|emb|CAA81951| (Z28111) ORF YKL112w
[Saccharomyces cerevisiae] >gi|1680183|gb||S93804_2
(S93804) ARS-binding factor I [Saccharomyces cerevisiae]
Length = 731
Score = 37.5 bits (85), Expect = 0.095
Identities = 45/183 (24%), Positives = 77/183 (41%), Gaps = 22/183 (12%)
Query: 686 VGMENTRYESVCEKSKDVESDKIKNLKYYKLPVNDLDGEY-------------KEDASLA 546
+ N + S D NL + +D+ G + +++ SL
Sbjct: 290 INNNNVGSHGISSHSPSSIRDTSMNLDVFNSATDDIPGPFVVTKIEPYHSHPLEDNLSLG 349
Query: 545 SALKKDIPVSSGDSHKKDLPLSGGISYSGKNIPKKCLTAIVEENTEYDGLVKVYPTTTSP 366
+ IP + K D L + S + K ++ VEE+ D L++ Y T
Sbjct: 350 KFILTKIPKILQNDLKFDQILESSYNNSNHTVSKFKVSHYVEESGLLDILMQRYGLTAED 409
Query: 365 ---KLLSMMTYFDNSAKPGFVTTKKKVG-FKDISNSGYTYGKNKKEDGWQYVMSRQKSID 198
+LLS + + K FV KKK+G + D+ S + N + R SID
Sbjct: 410 FEKRLLSQIARRITTYKARFVLKKKKMGEYNDLQPSSSSNNNNNNDGELSGTNLRSNSID 469
Query: 197 -----EIKSSAKTLNPWGRLKSSEN 138
EI S+ + N + +++N
Sbjct: 470 YAKHQEISSAGTSSNTTKNVNNNKN 494
>gi|114814|sp|P14164|BAF1_YEAST TRANSCRIPTION FACTOR BAF1 (ARS BINDING FACTOR 1) (PROTEIN ABF1)
(BIDIRECTIONALLY ACTING FACTOR) (SFB-B) (DNA REPLICATION
ENHANCER-BINDING PROTEIN OBF1) >gi|58415|emb|CAA35966|
(X51654) ARS-binding protein 1 [Saccharomyces
cerevisiae] >gi|170968 (M29067) ARS binding factor 1
[Saccharomyces cerevisiae] >gi|172058 (M63578) OBF1
[Saccharomyces cerevisiae] >gi|226720|prf||1604243A ARS
binding factor I [Saccharomyces cerevisiae]
Length = 731
Score = 37.5 bits (85), Expect = 0.095
Identities = 45/183 (24%), Positives = 77/183 (41%), Gaps = 22/183 (12%)
Query: 686 VGMENTRYESVCEKSKDVESDKIKNLKYYKLPVNDLDGEY-------------KEDASLA 546
+ N + S D NL + +D+ G + +++ SL
Sbjct: 290 INNNNVGSHGISSHSPSSIRDTSMNLDVFNSATDDIPGPFVVTKIEPYHSHPLEDNLSLG 349
Query: 545 SALKKDIPVSSGDSHKKDLPLSGGISYSGKNIPKKCLTAIVEENTEYDGLVKVYPTTTSP 366
+ IP + K D L + S + K ++ VEE+ D L++ Y T
Sbjct: 350 KFILTKIPKILQNDLKFDQILESSYNNSNHTVSKFKVSHYVEESGLLDILMQRYGLTAED 409
Query: 365 ---KLLSMMTYFDNSAKPGFVTTKKKVG-FKDISNSGYTYGKNKKEDGWQYVMSRQKSID 198
+LLS + + K FV KKK+G + D+ S + N + R SID
Sbjct: 410 FEKRLLSQIARRITTYKARFVLKKKKMGEYNDLQPSSSSNNNNNNDGELSGTNLRSNSID 469
Query: 197 -----EIKSSAKTLNPWGRLKSSEN 138
EI S+ + N + +++N
Sbjct: 470 YAKHQEISSAGTSSNTTKNVNNNKN 494
>gi|3914889|sp|O51453|RS13_BORBU 30S RIBOSOMAL PROTEIN S13 >gi|2688391 (AE001152) ribosomal protein
S13 (rpsM) [Borrelia burgdorferi]
Length = 125
Score = 34.4 bits (77), Expect = 0.82
Identities = 26/69 (37%), Positives = 41/69 (58%), Gaps = 10/69 (14%)
Query: 683 GMENTRYESVCEKS--------KDVESDKIKNLKYYKLPVNDLDGEYKEDASLASALKK- 531
G+ TR VC KS KD+++D++ L+ K+ +D E K + +A ++K+
Sbjct: 24 GIGRTRALEVCNKSSISPSKIAKDLDNDEVNRLR--KVIESDYIVEGKLRSEVAMSIKRL 81
Query: 530 -DIPVSSGDSHKKDLPLSG 477
DI G H+K LPL G
Sbjct: 82 MDIACYRGVRHRKGLPLRG 100
>gi|3861177|emb|CAA15077| (AJ235272) 30S RIBOSOMAL PROTEIN S13 (rpsM) [Rickettsia prowazekii]
Length = 125
Score = 32.5 bits (72), Expect = 3.2
Identities = 27/86 (31%), Positives = 42/86 (48%), Gaps = 8/86 (9%)
Query: 683 GMENTRYESVCEKSKDVESDKIKNLKYYKLPV--NDLDGEYK------EDASLASALKKD 528
G+ +T +C K+K + K+K L +L N ++ EYK + +L KKD
Sbjct: 24 GLGSTMAAEICNKAKISKDKKVKVLTDQELISLRNIIENEYKVEGDLKREVTLNIKKKKD 83
Query: 527 IPVSSGDSHKKDLPLSGGISYSGKNIPKKCLTAI 426
I G H + LP+ G ++S K AI
Sbjct: 84 IRCYQGLRHIRKLPVRGQNTHSNARTRKGKAIAI 117
>gi|309820 (L20871) Antigen [Pneumocystis carinii]
Length = 417
Score = 32.5 bits (72), Expect = 3.2
Identities = 29/89 (32%), Positives = 40/89 (44%), Gaps = 7/89 (7%)
Query: 653 CEKSKDVESDKIKNLKYYKLPVNDLDGEYKEDASLASALKKDIPVSSGDSHKK------D 492
C K ++ S+ K LK + L G K+D ALKK P + K D
Sbjct: 18 CLKLEEECSETFKKLKSKDKLTHLLKGNLKDDEKCKEALKKHCPELKNNETFKILLGECD 77
Query: 491 LPLSGGI-SYSGKNIPKKCLTAIVEENTEYDGLVKV 387
+ G + + K + KKCLT E N D L K+
Sbjct: 78 DNIKGNVCAELVKKVQKKCLTLKDELNKAKDELTKM 113
>gi|100901|pir||S19254 nitrate reductase (NADH) (EC 1.6.6.1) flavin chain (clone Zmnr1) -
maize (fragment)
Length = 617
Score = 32.1 bits (71), Expect = 4.2
Identities = 20/65 (30%), Positives = 33/65 (50%), Gaps = 3/65 (4%)
Query: 461 GKNIPKKCLTAIVEENTEYDGLVKVYPTTTSPKLLS---MMTYFDNSAKPGFVTTKKKVG 291
GK ++ T++V+E +D LVKVY PK + M Y D+ G++ K +G
Sbjct: 408 GKLCMRRTRTSMVDEIGHFDLLVKVYFKNEHPKFPNGGLMTQYLDSLPVGGYIDVKGPLG 467
Query: 290 FKDISNSG 267
+ + G
Sbjct: 468 HVEYTGRG 475
>gi|3024085|sp|Q28824|KMLS_BOVIN MYOSIN LIGHT CHAIN KINASE, SMOOTH MUSCLE (MLCK) (CONTAINS: TELOKIN)
>gi|484362|pir||JN0583 myosin-light-chain kinase (EC
2.7.1.117) 155K protein - bovine >gi|298639|bbs|127479
(S57131) 155 kda myosin light chain kinase homolog
[cattle, stomach, Peptide, 1176 aa] [Bos taurus]
Length = 1176
Score = 31.7 bits (70), Expect = 5.4
Identities = 18/45 (40%), Positives = 24/45 (53%), Gaps = 3/45 (6%)
Query: 182 AKTLNPWGRLKSSENP--FETIKPLKTPSPSEN-SPLESANPFKTTDP 48
A+T P G K +E P +KP +TP P N P E+ P +T P
Sbjct: 146 AETPKPLGSTKPAETPKPLGNVKPAETPKPLGNIKPTETPKPLGSTKP 193
Score = 31.3 bits (69), Expect = 7.1
Identities = 17/45 (37%), Positives = 24/45 (52%), Gaps = 3/45 (6%)
Query: 182 AKTLNPWGRLKSSENP--FETIKPLKTPSPSENS-PLESANPFKTTDP 48
A+T P G +K +E P +KP +TP P N+ P E+ P P
Sbjct: 218 AETPKPLGNVKPAETPKPLGNVKPAETPKPVSNAKPAETLKPVGNAKP 265
>gi|2961377|emb|CAA18124| (AL022141) putative receptor protein kinase [Arabidopsis thaliana]
Length = 1134
Score = 31.7 bits (70), Expect = 5.4
Identities = 22/67 (32%), Positives = 32/67 (46%), Gaps = 11/67 (16%)
Query: 620 IKNLKYYKLPVNDLDGEYKEDASLASA-----------LKKDIPVSSGDSHKKDLPLSGG 474
+ NL L VN+L GE +L S+ LK +IP S G SG
Sbjct: 665 LSNLTKMDLSVNNLTGEIPASLALISSNLVYFNVSSNNLKGEIPASLGSRINNTSEFSGN 724
Query: 473 ISYSGKNIPKKCLTAIVE 420
GK + ++C ++ E
Sbjct: 725 TELCGKPLNRRCESSTAE 742
>gi|902755 (U20460) hemagglutinin precursor [Influenza A virus]
Length = 568
Score = 31.3 bits (69), Expect = 7.1
Identities = 29/101 (28%), Positives = 45/101 (43%), Gaps = 2/101 (1%)
Query: 659 SVCEKSKDVESDKIKNLKYYKLPVNDLDGEYKEDASLASALKKD--IPVSSGDSHKKDLP 486
+V E S VE D N Y ND + E K S + +K IP SS +H
Sbjct: 88 NVPEWSYIVEKDNPINSLCYPGDFNDYE-ELKHLLSSTNHFEKIQIIPRSSWSNHDASSG 146
Query: 485 LSGGISYSGKNIPKKCLTAIVEENTEYDGLVKVYPTTTSPKLL 357
+S Y+G++ + + ++E+N Y + + Y T LL
Sbjct: 147 VSSACPYNGRSSFFRNVVWLIEKNNAYPTIKRSYNNTNQEDLL 189
>gi|1944199|dbj|BAA19658| (AB002817) dihydroflavonol 4-reductase [Perilla frutescens]
Length = 456
Score = 30.9 bits (68), Expect = 9.4
Identities = 21/79 (26%), Positives = 37/79 (46%)
Query: 404 DGLVKVYPTTTSPKLLSMMTYFDNSAKPGFVTTKKKVGFKDISNSGYTYGKNKKEDGWQY 225
DG++ + + T K + + F NSA V +K + + + S + +KK GW Y
Sbjct: 112 DGMLSIMRSCTKAKTVKKLI-FTNSAGTLNVEEHQKPVYNEANWSDLDFIYSKKMTGWMY 170
Query: 224 VMSRQKSIDEIKSSAKTLN 168
+S+ + E +AK N
Sbjct: 171 FVSKILAEKEAMKAAKESN 189
Database: Non-redundant GenBank CDS
translations+PDB+SwissProt+SPupdate+PIR
Posted date: Apr 12, 1999 12:54 PM
Number of letters in database: 112,640,273
Number of sequences in database: 368,476
Lambda K H
0.318 0.135 0.00
Gapped
Lambda K H
0.270 0.0470 4.94e-324
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 124639123
Number of Sequences: 368476
Number of extensions: 2382670
Number of successful extensions: 7993
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 12
Number of HSP's that attempted gapping in prelim test: 7985
Number of HSP's gapped (non-prelim): 20
length of query: 234
length of database: 112640273
effective HSP length: 53
effective length of query: 181
effective length of database: 93111045
effective search space: 16853099145
effective search space used: 16853099145
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 68 (30.9 bits)