BLASTX 2.0.8 [Jan-05-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 14VII-21F
(799 letters)
Database: Non-redundant GenBank CDS
translations+PDB+SwissProt+SPupdate+PIR
369,800 sequences; 113,023,754 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gi|1730840|sp|P53852|YNY7_YEAST PUTATIVE CYSTEINYL-TRNA SYNTHETA... 192 2e-48
gi|1351621|sp|Q09860|YAF6_SCHPO PUTATIVE CYSTEINYL-TRNA SYNTHETA... 175 3e-43
gi|3257043|dbj|BAA29726| (AP000003) 476aa long hypothetical cyst... 166 2e-41
gi|1711625|sp|P49589|SYC_HUMAN CYSTEINYL-TRNA SYNTHETASE (CYSTEI... 167 1e-40
gi|927229 (L06845) cysteinyl-tRNA synthetase [Homo sapiens] 167 1e-40
gi|3319446 (AF077541) contains similarity to class-I aminoacyl-t... 164 9e-40
gi|549024|sp|Q06752|SYC_BACSU CYSTEINYL-TRNA SYNTHETASE (CYSTEIN... 156 4e-39
gi|3242657|dbj|BAA29032| (AB015589) cysteinyl-tRNA synthetase [M... 160 1e-38
gi|2983551 (AE000721) cysteinyl-tRNA synthetase [Aquifex aeolicus] 138 5e-32
gi|3746059 (AC005311) putative cysteinyl-tRNA synthetase [Arabid... 138 5e-32
gi|41203|emb|CAA39691| (X56234) cysteine-tRNA ligase [Escherichi... 130 1e-30
gi|135097|sp|P21888|SYC_ECOLI CYSTEINYL-TRNA SYNTHETASE (CYSTEIN... 130 1e-30
gi|4377255|gb|AAD19070| (AE001673) Cysteinyl tRNA Synthetase [Ch... 131 6e-30
gi|3329246 (AE001350) Cysteinyl tRNA Synthetase [Chlamydia trach... 128 5e-29
gi|1174501|sp|P43816|SYC_HAEIN CYSTEINYL-TRNA SYNTHETASE (CYSTEI... 125 2e-28
gi|3122884|sp|O29836|SYC_ARCFU CYSTEINYL-TRNA SYNTHETASE (CYSTEI... 122 2e-27
gi|1351147|sp|P47495|SYC_MYCGE CYSTEINYL-TRNA SYNTHETASE (CYSTEI... 122 3e-27
gi|3860654|emb|CAA14555| (AJ235270) CYSTEINYL-TRNA SYNTHETASE (c... 122 4e-27
gi|2500966|sp|P74330|SYC_SYNY3 CYSTEINYL-TRNA SYNTHETASE (CYSTEI... 118 2e-26
gi|4155389 (AE001511) CYSTEINYL-TRNA SYNTHETASE [Helicobacter py... 116 2e-25
gi|2507427|sp|P41259|SYC_HELPY CYSTEINYL-TRNA SYNTHETASE (CYSTEI... 116 2e-25
gi|2500964|sp|P75423|SYC_MYCPN CYSTEINYL-TRNA SYNTHETASE (CYSTEI... 109 2e-23
gi|3122887|sp|P96862|SYC_MYCTU CYSTEINYL-TRNA SYNTHETASE (CYSTEI... 108 4e-23
gi|2688519 (AE001161) cysteinyl-tRNA synthetase (cysS) [Borrelia... 93 2e-18
gi|3322350 (AE001193) cysteinyl-tRNA synthetase (cysS) [Treponem... 87 1e-16
gi|3122878|sp|O07045|SYC_AZOBR CYSTEINYL-TRNA SYNTHETASE (CYSTEI... 84 1e-15
gi|3261823|emb|CAB10724| (Z97559) cysS2 [Mycobacterium tuberculo... 78 9e-14
gi|2181176|emb|CAA67901| (X99587) cysteine tRNA synthetase [Azos... 73 2e-12
gi|2145826|pir||S72854 cysteine--tRNA ligase (EC 6.1.1.16) - Myc... 57 2e-07
gi|3122899|sp|O30250|SYL_ARCFU LEUCYL-TRNA SYNTHETASE (LEUCINE--... 54 1e-06
gi|541386|pir||F53402 cysteine--tRNA ligase (EC 6.1.1.16) - Baci... 46 2e-06
gi|135140|sp|P10857|SYLC_NEUCR LEUCYL-TRNA SYNTHETASE, CYTOPLASM... 52 4e-06
gi|135141|sp|P26637|SYLC_YEAST LEUCYL-TRNA SYNTHETASE, CYTOPLASM... 48 6e-05
gi|171768 (M19992) isoleucyl-tRNA synthetase (EC 6.1.1.5) [Sacch... 46 4e-04
gi|1711638|sp|Q10490|SYLC_SCHPO PUTATIVE LEUCYL-TRNA SYNTHETASE,... 46 4e-04
gi|3257379|dbj|BAA30062| (AP000004) 967aa long hypothetical leuc... 43 0.003
gi|2129086|pir||A64379 isoleucine--tRNA ligase (EC 6.1.1.5) - Me... 43 0.003
gi|3915868|sp|Q58050|SYL_METJA LEUCYL-TRNA SYNTHETASE (LEUCINE--... 43 0.003
gi|628472|pir||C53739 vacA 5'-region protein - Helicobacter pylo... 42 0.004
gi|135134|sp|P09436|SYIC_YEAST ISOLEUCYL-TRNA SYNTHETASE, CYTOPL... 41 0.007
gi|1711637|sp|Q09996|SYLC_CAEEL PROBABLE LEUCYL-TRNA SYNTHETASE ... 41 0.010
gi|3874391|emb|CAA86751| (Z46787) similar to leucyl-tRNA synthet... 41 0.010
gi|135128|sp|P26499|SYI_METTM ISOLEUCYL-TRNA SYNTHETASE (ISOLEUC... 40 0.022
gi|68525|pir||SYEXI isoleucine--tRNA ligase (EC 6.1.1.5) - Metha... 40 0.022
gi|3122898|sp|O29622|SYI_ARCFU ISOLEUCYL-TRNA SYNTHETASE (ISOLEU... 39 0.029
gi|3334357|sp|O27428|SYI_METTH ISOLEUCYL-TRNA SYNTHETASE (ISOLEU... 39 0.029
gi|3122901|sp|O13651|SYIC_SCHPO ISOLEUCYL-TRNA SYNTHETASE, CYTOP... 39 0.029
gi|2984068 (AE000755) leucyl-tRNA synthetase beta subunit [Aquif... 39 0.038
gi|3122906|sp|O26861|SYV_METTH VALYL-TRNA SYNTHETASE (VALINE--TR... 39 0.049
gi|1174531|sp|P43827|SYL_HAEIN LEUCYL-TRNA SYNTHETASE (LEUCINE--... 38 0.064
gi|2983002 (AE000683) isoleucyl-tRNA synthetase [Aquifex aeolicus] 38 0.084
gi|2501013|sp|Q21926|SYI_CAEEL PROBABLE ISOLEUCYL-TRNA SYNTHETAS... 38 0.084
gi|2501065|sp|P56000|SYV_HELPY VALYL-TRNA SYNTHETASE (VALINE--TR... 38 0.084
gi|2160156 (AC000132) Strong similarity to S. pombe leucyl-tRNA ... 38 0.11
gi|2501068|sp|Q58413|SYV_METJA VALYL-TRNA SYNTHETASE (VALINE--TR... 37 0.14
gi|3861224|emb|CAA15124| (AJ235272) VALYL-TRNA SYNTHETASE (valS)... 37 0.19
gi|4155674 (AE001535) VALYL-TRNA SYNTHETASE [Helicobacter pylori... 37 0.19
gi|4504555|oth|NP_002152|PIARS isoleucine-tRNA synthetase >gi|44... 36 0.33
gi|2501012|sp|Q58357|SYI_METJA ISOLEUCYL-TRNA SYNTHETASE (ISOLEU... 36 0.33
gi|730870|sp|P41252|SYI_HUMAN ISOLEUCYL-TRNA SYNTHETASE, CYTOPLA... 36 0.33
gi|2688775 (AE001181) isoleucyl-tRNA synthetase (ileS) [Borrelia... 35 0.73
gi|4156066 (AE001567) LEUCYL-TRNA SYNTHETASE [Helicobacter pylor... 34 0.96
gi|3122908|sp|O27552|SYL_METTH LEUCYL-TRNA SYNTHETASE (LEUCINE--... 34 0.96
gi|1174521|sp|P41972|SYI_STAAU ISOLEUCYL-TRNA SYNTHETASE (ISOLEU... 34 0.96
gi|2293014|emb|CAA71688| (Y10687) purine NTPase [Sulfolobus acid... 34 1.3
gi|2447060 (U42580) A559L [Paramecium bursaria Chlorella virus 1] 34 1.3
gi|1711635|sp|Q10765|SYI_MYCTU PROBABLE ISOLEUCYL-TRNA SYNTHETAS... 34 1.3
gi|3024678|sp|P56457|SYL_HELPY LEUCYL-TRNA SYNTHETASE (LEUCINE--... 34 1.6
gi|3122909|sp|O28059|SYV_ARCFU VALYL-TRNA SYNTHETASE (VALINE--TR... 34 1.6
gi|3257481|dbj|BAA30164| (AP000004) 1066aa long hypothetical iso... 34 1.6
gi|2496245|sp|Q57611|Y147_METJA HYPOTHETICAL ATP-BINDING PROTEIN... 33 2.8
gi|2984142 (AE000760) preprotein translocase SecA subunit [Aquif... 33 2.8
gi|2688027 (AE001125) acriflavine resistance protein (acrB) [Bor... 32 3.7
gi|2688147 (AE001135) leucyl-tRNA synthetase (leuS) [Borrelia bu... 32 3.7
gi|4538961|emb|CAB39785| (AL049488) isoleucine-tRNA ligase-like ... 32 3.7
gi|2388946|emb|CAB11680| (Z98978) SPAC27E2.06c, putative methion... 32 4.8
gi|4539131|emb|CAB39575| (AL049478) putative isoleucyl-tRNA synt... 32 4.8
gi|1351159|sp|P47587|SYI_MYCGE ISOLEUCYL-TRNA SYNTHETASE (ISOLEU... 32 4.8
gi|2501026|sp|P75398|SYL_MYCPN LEUCYL-TRNA SYNTHETASE (LEUCINE--... 32 4.8
gi|3758845|emb|CAB11130| (Z98551) predicted using hexExon; MAL3P... 32 4.8
gi|2983452 (AE000714) arginyl-tRNA synthetase [Aquifex aeolicus] 32 4.8
gi|4155204 (AE001497) RNA POLYMERASE SIGMA-54 FACTOR [Helicobact... 32 4.8
gi|2496959|sp|Q09307|YQR2_CAEEL HYPOTHETICAL 45.1 KD PROTEIN F19... 32 6.3
gi|2507608|sp|P47508|SYL_MYCGE LEUCYL-TRNA SYNTHETASE (LEUCINE--... 32 6.3
gi|457146 (L27838) rhoptry protein [Plasmodium yoelii] 31 8.3
gi|3881411|emb|CAB00882| (Z77136) cDNA EST EMBL:C12394 comes fro... 31 8.3
gi|586388|sp|P38348|YB9Q_YEAST HYPOTHETICAL 55.5 KD PROTEIN IN M... 31 8.3
gi|780502 (U18466) AP endonuclease class II [African swine fever... 31 8.3
>gi|1730840|sp|P53852|YNY7_YEAST PUTATIVE CYSTEINYL-TRNA SYNTHETASE C29E6.06C (CYSTEINE--TRNA LIGASE)
(CYSRS) >gi|2132775|pir||S63220 probable membrane protein
YNL247w - yeast (Saccharomyces cerevisiae)
>gi|1255971|emb|CAA65497| (X96722) ORF N0885
[Saccharomyces cerevisiae] >gi|1302287|emb|CAA96154|
(Z71523) ORF YNL247w [Saccharomyces cerevisiae]
Length = 767
Score = 192 bits (484), Expect = 2e-48
Identities = 103/251 (41%), Positives = 159/251 (63%), Gaps = 24/251 (9%)
Query: 759 YESKWGEGRPGWHIECSAMAADIFGDELDIHTGAVDLKFPHHENEIHQCQAYFDKEPWVR 580
+ES WG+GRPGWHIECS MA+DI G +DIH+G +DL FPHH+NE+ Q +A FD + W+
Sbjct: 354 WESPWGKGRPGWHIECSVMASDILGSNIDIHSGGIDLAFPHHDNELAQSEARFDNQQWIN 413
Query: 579 YFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLNTPYNAPMTYKIESMEYA 400
YF+H+GHLHI+G KMSKSLKNFITI+E+LKK++ RQLR+ F + +N + +K +
Sbjct: 414 YFLHTGHLHIEGQKMSKSLKNFITIQEALKKFSPRQLRLAFASVQWNNQLDFKESLIHEV 473
Query: 399 KVQEARIFNFLSVAESKIKDNK---NIFKIMTMEDRMVVNMLIKAQKEVRDSFEDNFNTF 229
K E + NF + D +I K + ++ ++ ++++ +V +F DN +T
Sbjct: 474 KSFENSMNNFFKTIRALKNDAASAGHISKKFSPLEKELLADFVESESKVHSAFCDNLSTP 533
Query: 228 KTMSAILNLISVMNQKIDT----LTNSTILAVKNFIEKILRILGVITEEVN-----KTED 76
+ + L++ N I T L ++A+ ++I KILRI+G + N +
Sbjct: 534 VALKTLSELVTKSNTYITTAGAALKIEPLIAICSYITKILRIIGFPSRPDNLGWAAQAGS 593
Query: 75 ND------------VLDILGEYRNAIRKLAAKKKD 7
ND + L +R+ +R LA KK +
Sbjct: 594 NDGSLGSLEDTVMPYVKCLSTFRDDVRSLAIKKAE 628
>gi|1351621|sp|Q09860|YAF6_SCHPO PUTATIVE CYSTEINYL-TRNA SYNTHETASE C29E6.06C (CYSTEINE--TRNA LIGASE)
(CYSRS) >gi|1044932|emb|CAA91428| (Z66525) unknown
[Schizosaccharomyces pombe]
Length = 754
Score = 175 bits (439), Expect = 3e-43
Identities = 97/249 (38%), Positives = 156/249 (61%), Gaps = 25/249 (10%)
Query: 759 YESKWGEGRPGWHIECSAMAADIFGDELDIHTGAVDLKFPHHENEIHQCQAYFDKEPWVR 580
++S W +GRPGWHIECS MA+ + G +DIH+G +DL FPHH+NE+ Q +AYFD WV
Sbjct: 335 WDSPWSKGRPGWHIECSVMASALLGSNIDIHSGGIDLAFPHHDNELAQSEAYFDCPQWVN 394
Query: 579 YFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLNTPYNAPMTYKIESMEYA 400
YF H+GHLHI+G KMSKSLKNFITI+E LKK+T RQLR+ FL +N + +K + Y
Sbjct: 395 YFFHAGHLHIEGQKMSKSLKNFITIKEILKKFTPRQLRLAFLLQQWNTQLDFKETLLAYV 454
Query: 399 KVQEARIFNFLSVAESKIKDNKNIF----KIMTMEDRMVVNMLIKAQKEVRD---SFEDN 241
E + NF + + + + + + D++ + +L K Q+ ++ + D+
Sbjct: 455 LNIEQALENFFRTVRALMNETEGVSANGGHVPEKFDKLEIELLEKFQQTQQNTHLALCDS 514
Query: 240 FNTFKTMSAILNLISVMN---QKIDTLTNSTILA-VKNFIEKILRILGV----------- 106
FNT M I NL++ N +++ S +L + ++I +L+I G+
Sbjct: 515 FNTPLVMQHIDNLVTQANIYIREVGQQPCSRLLGQIASWITSMLQIFGLDENGHPNAVGW 574
Query: 105 ITEEVNKTEDNDVLDIL---GEYRNAIRKLAAKK 13
+ + + +E+ D + + +R+ +R+L K
Sbjct: 575 SSSKGSSSENTDAMPYIRAVSSFRDRVRELCIAK 608
>gi|3257043|dbj|BAA29726| (AP000003) 476aa long hypothetical cysteinyl-tRNA synthetase
[Pyrococcus horikoshii]
Length = 476
Score = 166 bits (417), Expect = 2e-41
Identities = 87/244 (35%), Positives = 140/244 (56%), Gaps = 6/244 (2%)
Query: 759 YESKWGEGRPGWHIECSAMAADIFGDELDIHTGAVDLKFPHHENEIHQCQAYFDKEPWVR 580
+ES WGEGRPGWHIECS M++ G+ DIH G DL FPHHENEI Q +A F E WV
Sbjct: 194 WESPWGEGRPGWHIECSVMSSKYLGESFDIHGGGNDLIFPHHENEIAQSEACFGHE-WVH 252
Query: 579 YFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLNTPYNAPMTYKIESMEYA 400
Y++H+G + + G KMSKSL NF+T+ E L++Y+ +R F L Y +P+ Y E +++A
Sbjct: 253 YWLHTGFVMVKGEKMSKSLGNFVTVRELLQRYSPEVIRFFVLQKHYRSPLDYSEEGLQHA 312
Query: 399 KVQEARIFNFLSVAESKIKDNKNIFKIMTMEDRMVVNMLIKAQKEVRDSFEDNFNTFKTM 220
K R++N L + +N + D N + +A+++ ++ +D+FNT + +
Sbjct: 313 KNNLERLYNTLENIRIAM-ENAELAYTWDENDFKAYNSIKEARRKFYEAMDDDFNTAEAL 371
Query: 219 SAILNLISVMNQKI---DTLTNSTILAVKNFIEKILRILGVITE---EVNKTEDNDVLDI 58
A+ + + +N+ I + S + F + I G+ E E E+ ++++
Sbjct: 372 KAVFEVSNAINKYITEAEKPKESVLRKAWEFFRTVGEIFGIFEEYFKEEKAKEEEKLIEL 431
Query: 57 LGEYRNAIRK 28
L + R +RK
Sbjct: 432 LIQVRAELRK 441
Score = 23.9 bits (50), Expect = 2e-41
Identities = 9/11 (81%), Positives = 9/11 (81%)
Query: 799 FVLWKKAKEGE 767
F LWKKAK GE
Sbjct: 181 FALWKKAKPGE 191
>gi|1711625|sp|P49589|SYC_HUMAN CYSTEINYL-TRNA SYNTHETASE (CYSTEINE--TRNA LIGASE) (CYSRS)
Length = 638
Score = 167 bits (418), Expect = 1e-40
Identities = 94/246 (38%), Positives = 146/246 (59%), Gaps = 24/246 (9%)
Query: 747 WGEGRPGWHIECSAMAADIFGDELDIHTGAVDLKFPHHENEIHQCQAYFDKEPWVRYFMH 568
WG+GRPGWHIECSAMA + G +DIH G DL+FPHH+NE+ Q +AYF+ + WVRYF+H
Sbjct: 337 WGKGRPGWHIECSAMAGTLLGASMDIHGGGFDLRFPHHDNELAQSEAYFENDCWVRYFLH 396
Query: 567 SGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLNTPYNAPMTYKIESMEYAKVQE 388
+GHL I G KMSKSLKNFITI+++LKK+++RQLR+ FL + + Y +ME A E
Sbjct: 397 TGHLTIAGCKMSKSLKNFITIKDALKKHSARQLRLAFLMHSWKDTLDYSSNTMESALQYE 456
Query: 387 ARIFNFLSVAESKIK---DNKNIFKIMTMEDRMVVNMLIKAQKEVRDSFEDNFNTFKTMS 217
+ F + ++ D F+ E+ + + + + DN +T M
Sbjct: 457 KFLNEFFLNVKDILRAPVDITGQFEKWGEEEAELNKNFYDKKTAIHKALCDNVDTRTVME 516
Query: 216 AILNLISVMN------QKIDTLTNSTILA-VKNFIEKILRILGVITEEVN---------- 88
+ L+S N + + N +L + ++ +L+I G + E+ +
Sbjct: 517 EMRALVSQCNLYMAARKAVRKRPNQALLENIALYLTHMLKIFGAVEEDSSLGFPVGGPGT 576
Query: 87 ----KTEDNDVLDILGEYRNAIRKLAAKKK 10
+ L +L E+R +RK+A ++K
Sbjct: 577 SLSLEATVMPYLQVLSEFREGVRKIAREQK 606
Score = 21.2 bits (43), Expect = 1e-40
Identities = 9/22 (40%), Positives = 12/22 (53%)
Query: 799 FVLWKKAKEGEIFL*IKMGRGK 734
F LWK +K GE G+G+
Sbjct: 320 FALWKASKPGEPSWPCPWGKGR 341
>gi|927229 (L06845) cysteinyl-tRNA synthetase [Homo sapiens]
Length = 595
Score = 167 bits (418), Expect = 1e-40
Identities = 94/246 (38%), Positives = 146/246 (59%), Gaps = 24/246 (9%)
Query: 747 WGEGRPGWHIECSAMAADIFGDELDIHTGAVDLKFPHHENEIHQCQAYFDKEPWVRYFMH 568
WG+GRPGWHIECSAMA + G +DIH G DL+FPHH+NE+ Q +AYF+ + WVRYF+H
Sbjct: 294 WGKGRPGWHIECSAMAGTLLGASMDIHGGGFDLRFPHHDNELAQSEAYFENDCWVRYFLH 353
Query: 567 SGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLNTPYNAPMTYKIESMEYAKVQE 388
+GHL I G KMSKSLKNFITI+++LKK+++RQLR+ FL + + Y +ME A E
Sbjct: 354 TGHLTIAGCKMSKSLKNFITIKDALKKHSARQLRLAFLMHSWKDTLDYSSNTMESALQYE 413
Query: 387 ARIFNFLSVAESKIK---DNKNIFKIMTMEDRMVVNMLIKAQKEVRDSFEDNFNTFKTMS 217
+ F + ++ D F+ E+ + + + + DN +T M
Sbjct: 414 KFLNEFFLNVKDILRAPVDITGQFEKWGEEEAELNKNFYDKKTAIHKALCDNVDTRTVME 473
Query: 216 AILNLISVMN------QKIDTLTNSTILA-VKNFIEKILRILGVITEEVN---------- 88
+ L+S N + + N +L + ++ +L+I G + E+ +
Sbjct: 474 EMRALVSQCNLYMAARKAVRKRPNQALLENIALYLTHMLKIFGAVEEDSSLGFPVGGPGT 533
Query: 87 ----KTEDNDVLDILGEYRNAIRKLAAKKK 10
+ L +L E+R +RK+A ++K
Sbjct: 534 SLSLEATVMPYLQVLSEFREGVRKIAREQK 563
Score = 21.2 bits (43), Expect = 1e-40
Identities = 9/22 (40%), Positives = 12/22 (53%)
Query: 799 FVLWKKAKEGEIFL*IKMGRGK 734
F LWK +K GE G+G+
Sbjct: 277 FALWKASKPGEPSWPCPWGKGR 298
>gi|3319446 (AF077541) contains similarity to class-I aminoacyl-tRNA synthetases
[Caenorhabditis elegans]
Length = 909
Score = 164 bits (410), Expect = 9e-40
Identities = 99/251 (39%), Positives = 149/251 (58%), Gaps = 33/251 (13%)
Query: 762 FYESKWGEGRPGWHIECSAMAADIFGDELDIHTGAVDLKFPHHENEIHQCQAYFDKEPWV 583
F+ S+WG GRPGWHIECS M++ I G +LDIH G DLKFPHH+NEI Q +A++D WV
Sbjct: 494 FWPSEWGNGRPGWHIECSVMSSAICGSKLDIHAGGFDLKFPHHDNEIAQVEAHYDDPHWV 553
Query: 582 RYFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLNTPYNAPMTYKIESMEY 403
YF+H G L I G+KMSKSLKNFITI ++L+ YT RQLR+ FL + + Y +ME
Sbjct: 554 NYFLHCGTLRIQGMKMSKSLKNFITIRKALEDYTPRQLRLLFLMHNWADVLDYSSSTMER 613
Query: 402 AKVQEARIFNFLSVAESKIK-----DNKNIFKIMTMEDRMVVNMLIKAQKEVRDSFEDNF 238
A E F + + ++ D ++ ++ ++ K + EV ++ D+
Sbjct: 614 ALQFEKITNEFFLLVKDFLRRHYKPDRSEGYQKFQGKELKLMEEFGKLKSEVHEALCDSV 673
Query: 237 NTFKTMSAILNLISVMN------QKIDTLTNSTILA-VKNFIEKILRILGVITEE----- 94
+T + LIS+ N +K + N +L + +I +L+I G I +
Sbjct: 674 DTRSVIEKFRELISLGNAYIVEKEKEGHVPNCLLLRNIAAYITNLLKIFGAIPQSNQEIG 733
Query: 93 -VNKTEDN---------------DVLDILGEYRNAIRKLAAKKK 10
V++ E N LD L ++R +R +A + K
Sbjct: 734 FVSEDECNGEGASTSFNKEAVVMPYLDALAQFREKVRLIAKEHK 777
>gi|549024|sp|Q06752|SYC_BACSU CYSTEINYL-TRNA SYNTHETASE (CYSTEINE--TRNA LIGASE) (CYSRS)
>gi|541406|pir||C53402 cysteine--tRNA ligase (EC
6.1.1.16) - Bacillus subtilis >gi|289284 (L14580)
cysteinyl-tRNA synthetase [Bacillus subtilis]
>gi|467482|dbj|BAA05328| (D26185) cysteinyl-tRNA
synthetase [Bacillus subtilis] >gi|499303|emb|CAA52167|
(X73989) cysteine--tRNA ligase [Bacillus subtilis]
>gi|2632361|emb|CAB11870| (Z99104) cysteinyl-tRNA
synthetase [Bacillus subtilis]
Length = 466
Score = 156 bits (390), Expect = 4e-39
Identities = 86/244 (35%), Positives = 137/244 (55%), Gaps = 2/244 (0%)
Query: 759 YESKWGEGRPGWHIECSAMAADIFGDELDIHTGAVDLKFPHHENEIHQCQAYFDKEPWVR 580
++S WG+GRPGWHIECSAM GD++DIH G DL FPHHENEI Q +A K + +
Sbjct: 194 WDSPWGKGRPGWHIECSAMVKKYLGDQIDIHAGGQDLTFPHHENEIAQSEALTGK-TFAK 252
Query: 579 YFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLNTPYNAPMTYKIESMEYA 400
Y++H+G+++ID KMSKSL NF+ + + +K++ + LR F L+ Y P+ Y E +E
Sbjct: 253 YWLHNGYINIDNEKMSKSLGNFVLVHDIIKQHDPQLLRFFMLSVHYRHPINYSEELLENT 312
Query: 399 KVQEARIFNFLSVAESKIKDNKNIFKIMTMEDRMVVNMLIKAQKEVRDSFEDNFNTFKTM 220
K F+ L A S ++ N +T +D + + + +K + +D+FNT +
Sbjct: 313 K----SAFSRLKTAYSNLQHRLNSSTNLTEDDDQWLEKVEEHRKAFEEEMDDDFNTANAI 368
Query: 219 SAILNLISVMNQKI--DTLTNSTILAVKNFIEKILRILGVITEEVNKTEDNDVLDILGEY 46
S + +L N + D + I A ++I+ +LG E + D ++ D++ E
Sbjct: 369 SVLFDLAKHANYYLQKDHTADHVITAFIEMFDRIVSVLGFSLGE-QELLDQEIEDLI-EK 426
Query: 45 RNAIRK 28
RN R+
Sbjct: 427 RNEARR 432
Score = 27.0 bits (58), Expect = 4e-39
Identities = 12/22 (54%), Positives = 14/22 (63%)
Query: 799 FVLWKKAKEGEIFL*IKMGRGK 734
F LWK AKEGEI G+G+
Sbjct: 181 FALWKAAKEGEISWDSPWGKGR 202
>gi|3242657|dbj|BAA29032| (AB015589) cysteinyl-tRNA synthetase [Mus musculus]
Length = 486
Score = 160 bits (400), Expect = 1e-38
Identities = 81/187 (43%), Positives = 114/187 (60%), Gaps = 4/187 (2%)
Query: 747 WGEGRPGWHIECSAMAADIFGDELDIHTGAVDLKFPHHENEIHQCQAYFDKEPWVRYFMH 568
WG+GRPGWHIECSAMA + G +DIH G DL+FPHH+NE+ Q +AYF+ + WVRYF+H
Sbjct: 292 WGKGRPGWHIECSAMAGTLLGASMDIHGGGFDLRFPHHDNELAQSEAYFENDCWVRYFLH 351
Query: 567 SGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLNTPYNAPMTYKIESME----YA 400
+GHL I G KMSKSLKNFITI+++LKK+++RQLR+ FL + + Y +ME Y
Sbjct: 352 TGHLTIAGCKMSKSLKNFITIKDALKKHSARQLRLAFLMHSWKDTLDYSSNTMESALQYE 411
Query: 399 KVQEARIFNFLSVAESKIKDNKNIFKIMTMEDRMVVNMLIKAQKEVRDSFEDNFNTFKTM 220
K N + + + D F+ E+ + + V ++ DN +T M
Sbjct: 412 KFMNEFFLNVKDILRAPV-DITGQFEKWEAEEVELNKNFYGKKTAVHEALCDNIDTRTVM 470
Query: 219 SAILNLISVMN 187
+ L+S N
Sbjct: 471 EEMRALVSQCN 481
Score = 21.2 bits (43), Expect = 1e-38
Identities = 9/22 (40%), Positives = 12/22 (53%)
Query: 799 FVLWKKAKEGEIFL*IKMGRGK 734
F LWK +K GE G+G+
Sbjct: 275 FALWKASKPGEPSWPCPWGKGR 296
>gi|2983551 (AE000721) cysteinyl-tRNA synthetase [Aquifex aeolicus]
Length = 495
Score = 138 bits (345), Expect = 5e-32
Identities = 91/244 (37%), Positives = 140/244 (57%), Gaps = 30/244 (12%)
Query: 759 YESKWGEGRPGWHIECSAMAADIFGDELDIHTGAVDLKFPHHENEIHQCQAYFDKEPWVR 580
++S WG+GRPGWH EC AM G+ +DIH G +DL FPHHENEI Q +A K P+ R
Sbjct: 194 WDSPWGKGRPGWHTECVAMVFKHLGETIDIHGGGLDLVFPHHENEIAQAEAITGK-PFAR 252
Query: 579 YFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLNTPYNAPMTYKIESMEYA 400
Y+MH+G + + G KMSKSL N++T+ E KY LR+ L T Y +P+ + E ME
Sbjct: 253 YWMHNGLVTVGGQKMSKSLGNYVTLREVYTKYHPDVLRLLVLFTHYRSPLDFSWEKMEET 312
Query: 399 KVQEARIFNFLSVAESKIKDNKNIFKIMTMEDRM-VVNMLIKAQKEVRDSF----EDNFN 235
R+ N I+D + + K+ +E + + L + KE ++F D+FN
Sbjct: 313 LKAYERLKN-------AIEDLELLKKLQVVESKEGGTHPLYEQVKEFEENFYASLSDDFN 365
Query: 234 TFKTMSAILNLISVMNQ---------KI-DTLTNSTILAVKNFIEKILRILGVITE---- 97
T + +S + L+ +N+ KI D ++ A K+ + + +I G++ +
Sbjct: 366 TPEALSHVYKLVGELNKVKNKAYSEGKITDRELSAYEFASKSLLNTMKKIFGLLEDLYPE 425
Query: 96 -----------EVNKTEDNDVLDILGEYRNAIRK 28
E + D ++ IL E RN RK
Sbjct: 426 CKVERVVERELEAGQVFDEKLIQILIEARNIARK 459
Score = 20.8 bits (42), Expect = 5e-32
Identities = 9/22 (40%), Positives = 12/22 (53%)
Query: 799 FVLWKKAKEGEIFL*IKMGRGK 734
F LWK +K GE G+G+
Sbjct: 181 FALWKASKAGEPAWDSPWGKGR 202
>gi|3746059 (AC005311) putative cysteinyl-tRNA synthetase [Arabidopsis
thaliana] >gi|4432812|gb|AAD20662| (AC006593) putative
cysteinyl-tRNA synthetase [Arabidopsis thaliana]
Length = 563
Score = 138 bits (344), Expect = 5e-32
Identities = 88/252 (34%), Positives = 137/252 (53%), Gaps = 7/252 (2%)
Query: 762 FYESKWGEGRPGWHIECSAMAADIFGDELDIHTGAVDLKFPHHENEIHQCQAYFDKEPWV 583
F+ES WG GRPGWHIECSAM+A G DIH G +DL FPHHENEI Q A D +
Sbjct: 255 FWESPWGRGRPGWHIECSAMSAAYLGYSFDIHGGGMDLVFPHHENEIAQSCAACDSSN-I 313
Query: 582 RYFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLNTPYNAPMTYKIESMEY 403
Y++H+G + +D KMSKSL NF TI + + Y LR+F + T Y +P+ Y +E
Sbjct: 314 SYWIHNGFVTVDSEKMSKSLGNFFTIRQVIDLYHPLALRLFLMGTHYRSPINYSDFLLES 373
Query: 402 AKVQEARIFNFLSVAESKIKDNKNIFKIMTMEDRMVVNMLIKAQKEVRDSFEDNFNTFKT 223
A + I+ L ES + + + F+ ++ + + + + E S D+ T T
Sbjct: 374 ASERIFYIYQTLHDCESALGEKDSTFENGSVPSDTLTS-INTFRTEFVASMSDDLLTPVT 432
Query: 222 MSAILNLISVMNQKIDT-------LTNSTILAVKNFIEKILRILGVITEEVNKTEDNDVL 64
++A+ + +N I T ++ A++ I +L ILG++ T ++VL
Sbjct: 433 LAAMSEPLKTINDLIHTRKGKKQARREESLKALETTIRDVLTILGLM-----PTSYSEVL 487
Query: 63 DILGEYRNAIRKLAAKKKD 7
+ L E A+++ K++D
Sbjct: 488 EQLKE--KALKRAGLKEED 504
>gi|41203|emb|CAA39691| (X56234) cysteine-tRNA ligase [Escherichia coli]
Length = 461
Score = 130 bits (324), Expect = 1e-30
Identities = 76/234 (32%), Positives = 126/234 (53%), Gaps = 8/234 (3%)
Query: 759 YESKWGEGRPGWHIECSAMAADIFGDELDIHTGAVDLKFPHHENEIHQCQAYFDKEPWVR 580
+ S WG GRPGWHIECSAM G+ DIH G DL FPHHENEI Q D + +V
Sbjct: 194 WPSPWGAGRPGWHIECSAMNCKQLGNHFDIHGGGSDLMFPHHENEIAQSTCAHDGQ-YVN 252
Query: 579 YFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLNTPYNAPMTYKIESMEYA 400
Y+MHSG + +D KMSKSL NF T+ + LK Y + +R F ++ Y + + Y E+++ A
Sbjct: 253 YWMHSGMVMVDREKMSKSLGNFFTVRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQA 312
Query: 399 KVQEARIFNFLSVAESKIKDNKNIFKIMTMEDRMVVNMLIKAQKEVRDSFEDNFNTFKTM 220
+ R++ L + + E R + ++ +D+FNT +
Sbjct: 313 RAAVERLYTALRGTDKTVAPAGG----EAFEARFI------------EAMDDDFNTPEAY 356
Query: 219 SAILNLISVMNQ-KIDTLTNSTILAVKNFIEKILRILGVITEEVN-------KTEDNDVL 64
S + ++ +N+ K + + + +A + + K+ +LG++ +E + +D++V
Sbjct: 357 SVLFDMAREVNRLKAEDMAAANAMA--SHLRKLSAVLGLLEQEPEAFLQSGAQADDSEVA 414
Query: 63 DI 58
+I
Sbjct: 415 EI 416
Score = 24.3 bits (51), Expect = 1e-30
Identities = 9/11 (81%), Positives = 10/11 (90%)
Query: 799 FVLWKKAKEGE 767
FVLWK +KEGE
Sbjct: 181 FVLWKMSKEGE 191
>gi|135097|sp|P21888|SYC_ECOLI CYSTEINYL-TRNA SYNTHETASE (CYSTEINE--TRNA LIGASE) (CYSRS)
>gi|68549|pir||YYEC cysteine--tRNA ligase (EC 6.1.1.16)
- Escherichia coli >gi|41206|emb|CAA41983| (X59293)
cysteinyl-tRNA synthetase [Escherichia coli] >gi|145692
(M59381) transfer RNA-Cys synthetase [Escherichia coli]
>gi|1773207 (U82664) cysteinyl-tRNA synthetase
[Escherichia coli] >gi|1786737 (AE000158) cysteine tRNA
synthetase [Escherichia coli] >gi|227964|prf||1714234A
Cys-tRNA synthetase [Escherichia coli]
Length = 461
Score = 130 bits (323), Expect = 1e-30
Identities = 76/234 (32%), Positives = 126/234 (53%), Gaps = 8/234 (3%)
Query: 759 YESKWGEGRPGWHIECSAMAADIFGDELDIHTGAVDLKFPHHENEIHQCQAYFDKEPWVR 580
+ S WG GRPGWHIECSAM G+ DIH G DL FPHHENEI Q D + +V
Sbjct: 194 WPSPWGAGRPGWHIECSAMNCKQLGNHFDIHGGGSDLMFPHHENEIAQSTCAHDGQ-YVN 252
Query: 579 YFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLNTPYNAPMTYKIESMEYA 400
Y+MHSG + +D KMSKSL NF T+ + LK Y + +R F ++ Y + + Y E+++ A
Sbjct: 253 YWMHSGMVMVDREKMSKSLGNFFTVRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQA 312
Query: 399 KVQEARIFNFLSVAESKIKDNKNIFKIMTMEDRMVVNMLIKAQKEVRDSFEDNFNTFKTM 220
+ R++ L + + E R + ++ +D+FNT +
Sbjct: 313 RAALERLYTALRGTDKTVAPAGG----EAFEARFI------------EAMDDDFNTPEAY 356
Query: 219 SAILNLISVMNQ-KIDTLTNSTILAVKNFIEKILRILGVITEEVN-------KTEDNDVL 64
S + ++ +N+ K + + + +A + + K+ +LG++ +E + +D++V
Sbjct: 357 SVLFDMAREVNRLKAEDMAAANAMA--SHLRKLSAVLGLLEQEPEAFLQSGAQADDSEVA 414
Query: 63 DI 58
+I
Sbjct: 415 EI 416
Score = 24.3 bits (51), Expect = 1e-30
Identities = 9/11 (81%), Positives = 10/11 (90%)
Query: 799 FVLWKKAKEGE 767
FVLWK +KEGE
Sbjct: 181 FVLWKMSKEGE 191
>gi|4377255|gb|AAD19070| (AE001673) Cysteinyl tRNA Synthetase [Chlamydia pneumoniae]
Length = 474
Score = 131 bits (326), Expect = 6e-30
Identities = 76/242 (31%), Positives = 130/242 (53%), Gaps = 3/242 (1%)
Query: 762 FYESKWGEGRPGWHIECSAMAADIFGDELDIHTGAVDLKFPHHENEIHQCQAYFDKEPWV 583
++ES +G+GRPGWH+ECS MA ++ GD LDIH G VD FPHHENEI Q +A K P+
Sbjct: 203 YWESPFGKGRPGWHLECSIMAMELLGDSLDIHAGGVDNIFPHHENEIAQSEALSGK-PFA 261
Query: 582 RYFMHSGHLHIDGLKMSKSLKNFITIEESL-KKYTSRQLRIFFLNTPYNAPMTYKIESME 406
RY++HS HL IDG KMSKSL NF+T+ + L +++T +++R L + Y + + E++
Sbjct: 262 RYWLHSEHLLIDGKKMSKSLGNFLTLRDLLHQEFTGQEVRYMLLQSHYRTQLNFTEEALL 321
Query: 405 YAKVQEARIFNFLSVAESKIKDNKNIFKIMTMEDRMVVNMLIKAQKEVRDSFEDNFNTFK 226
+ R+ +F+S E ++ + +A ++ N
Sbjct: 322 ACRHALRRLKDFVSRLEGVDLPGESPLPRTLDSSSQFIEAFSRA-------LANDLNVST 374
Query: 225 TMSAILNLISVMNQKIDT--LTNSTILAVKNFIEKILRILGVITEEVNKTEDNDVLDILG 52
+++ + + +N ID + + L + + ++K+ +LGV+ + V+ ++
Sbjct: 375 GFASLFDFVHEINTLIDQGHFSKADSLYILDTLKKVDTVLGVLPLTTSVCIPETVMQLVA 434
Query: 51 EYRNA 37
E A
Sbjct: 435 EREEA 439
>gi|3329246 (AE001350) Cysteinyl tRNA Synthetase [Chlamydia trachomatis]
Length = 497
Score = 128 bits (318), Expect = 5e-29
Identities = 82/251 (32%), Positives = 138/251 (54%), Gaps = 13/251 (5%)
Query: 777 RKERYFYESKWGEGRPGWHIECSAMAADIFGDELDIHTGAVDLKFPHHENEIHQCQAYFD 598
R F+ES +G+GRPGWH+ECS M+ + G LDIH G VD FPHHENEI Q ++
Sbjct: 212 RDGEIFWESPFGKGRPGWHLECSIMSISLLGQSLDIHAGGVDNIFPHHENEIAQSESLSH 271
Query: 597 KEPWVRYFMHSGHLHIDGLKMSKSLKNFITIEESLKK-YTSRQLRIFFLNTPYNAPMTYK 421
K P+VRY++HS HL +D KMSKSL NF T+ + L + ++ ++R L Y + +
Sbjct: 272 K-PFVRYWLHSHHLLVDRKKMSKSLGNFFTLRDLLDQGFSGEEVRYLLLQGHYRTQLNFT 330
Query: 420 IESMEYAKVQEARIFNFLSVAESKIKDNKNIFKIMTMEDRMVVNMLIKAQKEVRDSFEDN 241
E + ++ R+ +F+ E + I + + + I S ++
Sbjct: 331 QEGLHASRQSLKRLRDFICRLEDPSYPDDIIHPEVATACQSFLETFI-------TSLTND 383
Query: 240 FNTFKTMSAILNLISVMNQKID------TLTNSTILAVKN------FIEKILRILGVITE 97
N +++A+ + I +N ID T T+S++ + ++ + KI ++LGV+
Sbjct: 384 LNISSSLAALFDFIRKINSSIDQHTGIQTETDSSVFSKQDAQHILALLRKIDQVLGVL-- 441
Query: 96 EVNKTEDNDVLDILGEYRNAIRKL 25
++ + + + +L E R A RK+
Sbjct: 442 PFSQPDIPEEVLLLVEQREAARKV 465
>gi|1174501|sp|P43816|SYC_HAEIN CYSTEINYL-TRNA SYNTHETASE (CYSTEINE--TRNA LIGASE) (CYSRS)
>gi|1073961|pir||C64047 cysteine--tRNA ligase (EC
6.1.1.16) - Haemophilus influenzae (strain Rd KW20)
>gi|1573026 (U32693) cysteinyl-tRNA synthetase (cysS)
[Haemophilus influenzae Rd]
Length = 459
Score = 125 bits (311), Expect = 2e-28
Identities = 82/243 (33%), Positives = 129/243 (52%), Gaps = 9/243 (3%)
Query: 759 YESKWGEGRPGWHIECSAMAADIFGDELDIHTGAVDLKFPHHENEIHQ-CQAYFDKEPWV 583
+ S WG GRPGWHIECSAM G+ DIH G DL FPHHENEI Q C A+ + +V
Sbjct: 194 WASPWGAGRPGWHIECSAMNCKQLGEYFDIHGGGSDLMFPHHENEIAQSCCAHGGQ--YV 251
Query: 582 RYFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLNTPYNAPMTYKIESMEY 403
Y++HSG + +D KMSKSL NF TI + L Y + +R F L Y + + Y E++
Sbjct: 252 NYWIHSGMIMVDKEKMSKSLGNFFTIRDVLNHYNAEAVRYFLLTAHYRSQLNYSEENLNL 311
Query: 402 AKVQEARIFNFLSVAESKIKDNKNIFKIMTMEDRMVVNMLIKAQKEVRDSFEDNFNTFKT 223
A+ R++ L + + + V R++ +D+FNT
Sbjct: 312 AQGALERLYTALRGTDQS--------AVAFGGENFVATF--------REAMDDDFNTPNA 355
Query: 222 MSAILNLISVMNQ-KIDTLTNSTILAVKNFIEKILRILGVITEEVNK-----TEDNDV-- 67
+S + + +N+ K + + + LA + + ++ ILG++ +E K + D++V
Sbjct: 356 LSVLFEMAREINKLKTEDVEKANGLAAR--LRELGAILGLLQQEPEKFLQAGSNDDEVAK 413
Query: 66 LDILGEYRNAIR 31
++ L + RN R
Sbjct: 414 IEALIKQRNEAR 425
Score = 21.9 bits (45), Expect = 2e-28
Identities = 8/11 (72%), Positives = 9/11 (81%)
Query: 799 FVLWKKAKEGE 767
FVLWK +KE E
Sbjct: 181 FVLWKMSKENE 191
>gi|3122884|sp|O29836|SYC_ARCFU CYSTEINYL-TRNA SYNTHETASE (CYSTEINE--TRNA LIGASE) (CYSRS)
>gi|2650218 (AE001076) cysteinyl-tRNA synthetase (cysS)
[Archaeoglobus fulgidus]
Length = 467
Score = 122 bits (304), Expect = 2e-27
Identities = 82/224 (36%), Positives = 113/224 (49%), Gaps = 1/224 (0%)
Query: 774 KERYFYESKWGEGRPGWHIECSAMAADIFGDELDIHTGAVDLKFPHHENEIHQCQAYFDK 595
K + ++S WG GRPGWHIECS M+A G DIH G DL FPHHENE Q A F
Sbjct: 191 KAQAVFDSPWGRGRPGWHIECSVMSAKYLGVPFDIHGGGKDLIFPHHENERAQSFARFGV 250
Query: 594 EPWVRYFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLNTPYNAPMTYKIE 415
EP V+ ++H+ + I G KMSKSL N + I + L++Y LR F L Y +P+ Y E
Sbjct: 251 EP-VKIWVHNDFIRIKGEKMSKSLGNIVRIRDVLQRYEGEVLRYFLLTAHYRSPLDYTEE 309
Query: 414 SMEYAKVQEARIFNFLSVAESKIKDNKNIFKIMTMEDRMVVNMLIKAQKEVRDSFEDNFN 235
++E AK R + +L A I + I + T DR N + + +R FE+ N
Sbjct: 310 ALERAK----RAYEYLRSA--LINLDMEIAYLKTFGDRKEGNQ-VDVEDYIR-RFEEAMN 361
Query: 234 -TFKTMSAILNLISVMNQKIDTLTNSTILAVKNFIEKILRILGVI 103
T AI L +L ++ + E R+ GV+
Sbjct: 362 RDLHTPDAIAVLHEFAGLINKSLYELSLNQAEELYEAFKRLCGVL 406
Score = 21.6 bits (44), Expect = 2e-27
Identities = 7/12 (58%), Positives = 9/12 (74%)
Query: 799 FVLWKKAKEGEI 764
F LWK AKE ++
Sbjct: 179 FALWKSAKEADL 190
>gi|1351147|sp|P47495|SYC_MYCGE CYSTEINYL-TRNA SYNTHETASE (CYSTEINE--TRNA LIGASE) (CYSRS)
>gi|1361470|pir||I64227 cysteine--tRNA ligase (EC
6.1.1.16) - Mycoplasma genitalium (SGC3) >gi|3844851
(U39704) cysteinyl-tRNA synthetase (cysS) [Mycoplasma
genitalium]
Length = 428
Score = 122 bits (303), Expect = 3e-27
Identities = 77/224 (34%), Positives = 115/224 (50%)
Query: 759 YESKWGEGRPGWHIECSAMAADIFGDELDIHTGAVDLKFPHHENEIHQCQAYFDKEPWVR 580
+ SKWG GRPGWH+EC+ + F +EL IH G VDLKFPHHENE A ++ +P +
Sbjct: 181 WNSKWGLGRPGWHVECAFLIDYCFKNELTIHGGGVDLKFPHHENENALHMALYN-QPITK 239
Query: 579 YFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLNTPYNAPMTYKIESMEYA 400
++MH GHL I+ KMSKSL+NF+ + L + R LR F Y P+ +E A
Sbjct: 240 HWMHIGHLMIENQKMSKSLQNFLLAVDFLNFHDFRVLRWIFYQKHYLHPIDLNQSLIEKA 299
Query: 399 KVQEARIFNFLSVAESKIKDNKNIFKIMTMEDRMVVNMLIKAQKEVRDSFEDNFNTFKTM 220
RI L+VA + + ++ I + V + L+ DN N +
Sbjct: 300 NNDIQRIAKTLNVARTWLVYSEQSELISPKQYDPVFSALL-----------DNLNFANAV 348
Query: 219 SAILNLISVMNQKIDTLTNSTILAVKNFIEKILRILGVITEEVN 88
+AI LI +N I T S + +F+E + +LG+ + ++
Sbjct: 349 AAIWKLIKKINTSIKTKDFSVLREQLSFLEWSIDLLGISFKSIH 392
>gi|3860654|emb|CAA14555| (AJ235270) CYSTEINYL-TRNA SYNTHETASE (cysS) [Rickettsia prowazekii]
Length = 457
Score = 122 bits (302), Expect = 4e-27
Identities = 56/130 (43%), Positives = 81/130 (62%), Gaps = 1/130 (0%)
Query: 786 RRRRKERYFYESKWGEGRPGWHIECSAMAADIFGDELDIHTGAVDLKFPHHENEIHQCQA 607
++ E + S WG GRPGWHIECSAM+ G DIH G DL FPHH NEI Q +
Sbjct: 190 KKNEPENINFTSPWGLGRPGWHIECSAMSYKYLGQNFDIHGGGADLIFPHHTNEIAQSRC 249
Query: 606 YFDKEPWVRYFMHSGHLHIDGLKMSKSLKNFITIEESL-KKYTSRQLRIFFLNTPYNAPM 430
F + +Y++H+G L ++G KMSKSL NFIT+ + + K+ +R+F L++ Y P+
Sbjct: 250 AFPSSTYAKYWIHNGFLTVNGSKMSKSLGNFITVRDLMDKQIQGEVVRLFLLSSHYRRPL 309
Query: 429 TYKIESMEYAK 397
Y ++++ AK
Sbjct: 310 DYNDKAIDDAK 320
>gi|2500966|sp|P74330|SYC_SYNY3 CYSTEINYL-TRNA SYNTHETASE (CYSTEINE--TRNA LIGASE) (CYSRS)
>gi|1653511|dbj|BAA18424| (D90914) cysteinyl-tRNA
synthetase [Synechocystis sp.]
Length = 483
Score = 118 bits (292), Expect = 2e-26
Identities = 54/112 (48%), Positives = 72/112 (64%)
Query: 759 YESKWGEGRPGWHIECSAMAADIFGDELDIHTGAVDLKFPHHENEIHQCQAYFDKEPWVR 580
+ES WG+GRPGWHIECSAM + G+ +D+H G DL FPHHENEI Q +A +P +
Sbjct: 200 WESPWGKGRPGWHIECSAMVREFLGETIDLHVGGNDLIFPHHENEIAQSEA-VTGQPLAQ 258
Query: 579 YFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLNTPYNAPMTY 424
Y++H+G + +DG KMSKSL NF TI LK +R+ L Y P+ +
Sbjct: 259 YWLHNGMVKVDGEKMSKSLGNFTTIRALLKTVDPMAIRLLVLQGHYRKPLDF 310
Score = 22.3 bits (46), Expect = 2e-26
Identities = 10/22 (45%), Positives = 12/22 (54%)
Query: 799 FVLWKKAKEGEIFL*IKMGRGK 734
F LWK AK GE G+G+
Sbjct: 187 FALWKSAKPGEPAWESPWGKGR 208
>gi|4155389 (AE001511) CYSTEINYL-TRNA SYNTHETASE [Helicobacter pylori J99]
Length = 465
Score = 116 bits (288), Expect = 2e-25
Identities = 75/215 (34%), Positives = 117/215 (53%), Gaps = 15/215 (6%)
Query: 759 YESKWGEGRPGWHIECSAMAADIFG-----DELDIHTGAVDLKFPHHENEIHQCQAYFDK 595
++S G+GRPGWHIECS+M + ++DIH G DL FPHHENE Q + F
Sbjct: 192 FDSPLGKGRPGWHIECSSMVFETLALANAPYQIDIHAGGTDLLFPHHENEACQTRCAFGV 251
Query: 594 EPWVRYFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLNTPYNAPMTYKIE 415
E +Y+MH+G ++I+ KMSKSL N I+++LK Y LR + L Y + + + E
Sbjct: 252 EI-AKYWMHNGFVNINNEKMSKSLGNSFFIKDALKNYDGEILRNYLLGVHYRSVLNFNEE 310
Query: 414 SMEYAKVQEARIFNFLSVAESKIKDNKNIFKIMTMEDRMV-----VNMLIKAQKEVRDSF 250
+ +K K + KI ++ R++ +N K KE+ +
Sbjct: 311 DLLMSK--------------------KRLDKIYRLKQRVLGTLGGINPNFK--KEILECM 348
Query: 249 EDNFNTFKTMSAILNLISVMNQKID-----TLTNSTILAVKNFIEKILRI 115
+D+ N K +S + +++S N+K+D ILA FIE++L I
Sbjct: 349 QDDLNVSKALSVLESMLSSTNEKLDQNPKNKALKGEILANLKFIEELLGI 398
>gi|2507427|sp|P41259|SYC_HELPY CYSTEINYL-TRNA SYNTHETASE (CYSTEINE--TRNA LIGASE) (CYSRS)
>gi|2314022|gb|AAD07934.1| (AE000598) cysteinyl-tRNA
synthetase (cysS) [Helicobacter pylori 26695]
Length = 465
Score = 116 bits (288), Expect = 2e-25
Identities = 74/215 (34%), Positives = 117/215 (54%), Gaps = 15/215 (6%)
Query: 759 YESKWGEGRPGWHIECSAMAADIFG-----DELDIHTGAVDLKFPHHENEIHQCQAYFDK 595
++S G+GRPGWHIECS+M + ++DIH G DL FPHHENE Q + F
Sbjct: 192 FDSPLGKGRPGWHIECSSMVFETLALTNTPYQIDIHAGGADLLFPHHENEACQTRCAFGV 251
Query: 594 EPWVRYFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLNTPYNAPMTYKIE 415
E +Y+MH+G ++I+ KMSKSL N ++++LK Y LR + L Y + + + E
Sbjct: 252 E-LAKYWMHNGFVNINNEKMSKSLGNSFFVKDALKNYDGEILRNYLLGVHYRSVLNFNEE 310
Query: 414 SMEYAKVQEARIFNFLSVAESKIKDNKNIFKIMTMEDRMV-----VNMLIKAQKEVRDSF 250
+ +K K + KI ++ R++ +N K KE+ +
Sbjct: 311 DLLVSK--------------------KRLDKIYRLKQRVLGTLGGINPNFK--KEILECM 348
Query: 249 EDNFNTFKTMSAILNLISVMNQKID-----TLTNSTILAVKNFIEKILRI 115
+D+ N K +S + +++S N+K+D ILA FIE++L I
Sbjct: 349 QDDLNVSKALSVLESMLSSTNEKLDQNPKNKALKGEILANLKFIEELLGI 398
>gi|2500964|sp|P75423|SYC_MYCPN CYSTEINYL-TRNA SYNTHETASE (CYSTEINE--TRNA LIGASE) (CYSRS)
>gi|2146088|pir||S73806 cysteine--tRNA ligase (EC
6.1.1.16) cysS - Mycoplasma pneumoniae (SGC3) (ATCC
29342) >gi|1674171 (AE000047) Mycoplasma pneumoniae,
cysteinyl-tRNA synthetase; similar to Swiss-Prot
Accession Number Q06752, from B. subtilis [Mycoplasma
pneumoniae]
Length = 437
Score = 109 bits (271), Expect = 2e-23
Identities = 72/218 (33%), Positives = 105/218 (48%), Gaps = 3/218 (1%)
Query: 759 YESKWGEGRPGWHIECSAMAADIFGDELDIHTGAVDLKFPHHENEIHQCQAYFDKEPWVR 580
+ S WG GRPGWH+EC+ + F D+L IH G VDLKFPHHENE A +DK P +
Sbjct: 190 WNSPWGWGRPGWHVECAFLIDYSFKDQLTIHGGGVDLKFPHHENENAMHMALYDK-PLTQ 248
Query: 579 YFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLNTPYNAPMTYKIESMEYA 400
++MH GHL + KMSKSL+NF+ + L + R LR F Y P+ +E A
Sbjct: 249 HWMHIGHLMFENQKMSKSLQNFLLAVDFLTIHDFRILRWLFYQKHYYHPLDLSQSLIEQA 308
Query: 399 KVQEARIFNFLSVAESKIKDNKNIFKIMTMEDRMVVNMLIKAQKEVRDSFE---DNFNTF 229
RI ++V + ++ I A K+ F+ +N N
Sbjct: 309 CSDIKRIQKAVNVCRTWFVYSE--------------QSAIPAPKQFEPVFKALLNNLNFA 354
Query: 228 KTMSAILNLISVMNQKIDTLTNSTILAVKNFIEKILRILGV 106
++ I L+ +N + S + + +E L ILG+
Sbjct: 355 NAITHIWKLVKQINHDVSKQNLSGLKEHLSHLEWALNILGI 395
>gi|3122887|sp|P96862|SYC_MYCTU CYSTEINYL-TRNA SYNTHETASE (CYSTEINE--TRNA LIGASE) (CYSRS)
>gi|1877311|emb|CAB07154| (Z92774) cysS [Mycobacterium
tuberculosis]
Length = 469
Score = 108 bits (267), Expect = 4e-23
Identities = 53/138 (38%), Positives = 82/138 (59%)
Query: 759 YESKWGEGRPGWHIECSAMAADIFGDELDIHTGAVDLKFPHHENEIHQCQAYFDKEPWVR 580
+ + WG GRPGWH+ECSAMA G E DIH G +DL FPHHENEI Q +A D + R
Sbjct: 196 WPTPWGRGRPGWHLECSAMARSYLGPEFDIHCGGMDLVFPHHENEIAQSRAAGD--GFAR 253
Query: 579 YFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLNTPYNAPMTYKIESMEYA 400
Y++H+G + + G KMSKSL N +++ L++ +LR + + Y + + + +M+ A
Sbjct: 254 YWLHNGWVTMGGEKMSKSLGNVLSMPAMLQRVRPAELRYYLGSAHYRSMLEFSETAMQDA 313
Query: 399 KVQEARIFNFLSVAESKI 346
+ +FL +++
Sbjct: 314 VKAYVGLEDFLHRVRTRV 331
Score = 21.2 bits (43), Expect = 4e-23
Identities = 10/22 (45%), Positives = 11/22 (49%)
Query: 799 FVLWKKAKEGEIFL*IKMGRGK 734
F LWK K GE GRG+
Sbjct: 183 FTLWKGEKPGEPSWPTPWGRGR 204
>gi|2688519 (AE001161) cysteinyl-tRNA synthetase (cysS) [Borrelia burgdorferi]
Length = 480
Score = 93.2 bits (228), Expect = 2e-18
Identities = 65/232 (28%), Positives = 121/232 (52%), Gaps = 5/232 (2%)
Query: 759 YESKWGEGRPGWHIECSAMAADIFGDELDIHTGAVDLKFPHHENEIHQCQAYFDKEPWVR 580
++S WG G P WH+EC+AM + F D LDIH G VD HH NEI + + +K+ W
Sbjct: 206 WDSPWGFGYPSWHLECAAMNLEYFKDALDIHLGGVDHIGVHHINEIAIAECFLNKK-WCD 264
Query: 579 YFMHSGHLHIDGLKMSKSLKNFITIEE-SLKKYTSRQLRIFFLNTPYNAPMTYKIESMEY 403
F+H L +D KMSKS NFIT+++ + ++ R L + Y + + +++++
Sbjct: 265 VFVHGEFLIMDYNKMSKSRGNFITVKDLEDQNFSPLDFRYLCLTSHYRNQLKFSLDNLQA 324
Query: 402 AKVQEARIFNFLSVAESKIKDNKNIFKIMTMEDRMVVNMLIKAQKEVRDSFED----NFN 235
+K+ + N LS ++ + + T+ ++ + N +KE DSF + + N
Sbjct: 325 SKIARENLINKLSY----FYESLDPVDLNTL-NKDLKNFGFSVEKEYYDSFVEKISFDLN 379
Query: 234 TFKTMSAILNLISVMNQKIDTLTNSTILAVKNFIEKILRILGVITEEVNKTEDNDVL 64
+ ++ + +I K D L+ + L + ++I+ L + E + +++DV+
Sbjct: 380 VAQGLALLWEII-----KSDNLSFVSKLRLAFIFDEIMS-LNLREEILKNLQNHDVV 430
>gi|3322350 (AE001193) cysteinyl-tRNA synthetase (cysS) [Treponema pallidum]
Length = 520
Score = 87.0 bits (212), Expect = 1e-16
Identities = 61/187 (32%), Positives = 96/187 (50%), Gaps = 26/187 (13%)
Query: 759 YESKWGEGRPGWHIECSAMAADIFGDELDIHTGAVDLKFPHHENEIHQCQAYFDKEPWVR 580
++S WG G PGWHI CSAM+ G DIH G VD HH NE QC+A PWVR
Sbjct: 212 WDSPWGRGYPGWHIGCSAMSMKFLGPRCDIHIGGVDHIRVHHRNERAQCEA-ITGAPWVR 270
Query: 579 YFMHSGHL---------HID--------GLKMSKSLKNFITIEESLKK-YTSRQLRIFFL 454
Y++H L H D KMSKS F+T+ ++ + R F L
Sbjct: 271 YWLHHEFLLMQLQKRAVHADMGSSVVSSFSKMSKSCGQFLTLSSLQERGFQPADFRFFLL 330
Query: 453 NTPYNAPMTYKIESMEYAKV-------QEARIFNFLSVAESKIK-DNKNIFKIMTMEDRM 298
+ Y + + ++++ A+ + AR+ + ++ + E R
Sbjct: 331 SGQYRTQLAFSWDALKTARAARRSFVRRVARVVDAARATTGSVRGTSAECAAERVCESRA 390
Query: 297 VVNMLIKAQKEVRDSFEDNFNTFKTMSAILNLI 199
+ L+ + R + ED+F+T + +SA+ L+
Sbjct: 391 SESELLLT--DFRAALEDDFSTPRALSALQKLV 421
>gi|3122878|sp|O07045|SYC_AZOBR CYSTEINYL-TRNA SYNTHETASE (CYSTEINE--TRNA LIGASE) (CYSRS)
Length = 448
Score = 83.9 bits (204), Expect = 1e-15
Identities = 42/83 (50%), Positives = 51/83 (60%), Gaps = 1/83 (1%)
Query: 759 YESKWGEGRPGWHIECSAMAADIFGDELDIHTGAVDLKFPHHENEIHQCQAYFDKEPWVR 580
++S WG GRPGWHIECSAMA + G DIH G +DL P HENEI Q + EP R
Sbjct: 191 WDSPWGRGRPGWHIECSAMAKEHLGVTFDIHGGGLDLILPDHENEIAQSRCAHAGEPLAR 250
Query: 579 YFMHSGHLHIDGL-KMSKSLKNFI 511
Y++H G +MSKSL N +
Sbjct: 251 YWVHQRLRDRRGARRMSKSLGNLL 274
>gi|3261823|emb|CAB10724| (Z97559) cysS2 [Mycobacterium tuberculosis]
Length = 414
Score = 77.6 bits (188), Expect = 9e-14
Identities = 35/85 (41%), Positives = 50/85 (58%)
Query: 759 YESKWGEGRPGWHIECSAMAADIFGDELDIHTGAVDLKFPHHENEIHQCQAYFDKEPWVR 580
+ S +G GRPGWH+EC+A+A G LDI G DL FPHHE + + + R
Sbjct: 218 WPSPFGPGRPGWHVECAAIALSRIGSGLDIQGGGSDLIFPHHEFTAAHAECVSGERRFAR 277
Query: 579 YFMHSGHLHIDGLKMSKSLKNFITI 505
+++H+G + DG KMSKS N + +
Sbjct: 278 HYVHAGMIGWDGHKMSKSRGNLVLV 302
>gi|2181176|emb|CAA67901| (X99587) cysteine tRNA synthetase [Azospirillum brasilense]
Length = 448
Score = 73.0 bits (176), Expect = 2e-12
Identities = 33/72 (45%), Positives = 42/72 (57%)
Query: 759 YESKWGEGRPGWHIECSAMAADIFGDELDIHTGAVDLKFPHHENEIHQCQAYFDKEPWVR 580
++S WG GRPGWHIECSAMA + G DIH G +DL P HENEI Q + W
Sbjct: 191 WDSPWGRGRPGWHIECSAMAKEHLGVTFDIHGGGLDLILPDHENEIAQSRCAHAGSRWPA 250
Query: 579 YFMHSGHLHIDG 544
+G + ++G
Sbjct: 251 TGSTNGFVTVEG 262
>gi|2145826|pir||S72854 cysteine--tRNA ligase (EC 6.1.1.16) - Mycobacterium leprae
>gi|467009 (U00017) syC; B2126_C2_193 [Mycobacterium
leprae]
Length = 241
Score = 57.0 bits (135), Expect = 2e-07
Identities = 24/43 (55%), Positives = 29/43 (66%)
Query: 759 YESKWGEGRPGWHIECSAMAADIFGDELDIHTGAVDLKFPHHE 631
+ S +G GRPGWH+EC+A+A G LDI G DL FPHHE
Sbjct: 176 WSSPFGPGRPGWHVECAAIALSRIGIGLDIQGGGSDLIFPHHE 218
>gi|3122899|sp|O30250|SYL_ARCFU LEUCYL-TRNA SYNTHETASE (LEUCINE--TRNA LIGASE) (LEURS) >gi|2650668
(AE001108) leucyl-tRNA synthetase (leuS) [Archaeoglobus
fulgidus]
Length = 932
Score = 54.3 bits (128), Expect = 1e-06
Identities = 45/177 (25%), Positives = 91/177 (50%), Gaps = 12/177 (6%)
Query: 744 GEGRPGWHIECSAMAADIFGD---------ELDIHTGAVDLKFPHHENEIHQCQAYFDKE 592
G+G G E S ++ ++ +D+ + DL H + A F +
Sbjct: 553 GKGEVGKVAEASKLSVELIQQIRDDFEYWYPVDLRSSGKDLVANHLLFYLFHHVAIFPPD 612
Query: 591 PWVRYFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLN-TPYNAPMTYKIE 415
W R +G++ ++G KMSKS +T++ ++++Y + R++ L+ Y++ +K
Sbjct: 613 KWPRAIAVNGYVSLEGKKMSKSKGPLLTMKRAVQQYGADVTRLYILHAAEYDSDADWKSR 672
Query: 414 SMEYAKVQEARIFNFLSVAESKIKDN--KNIFKIMTMEDRMVVNMLIKAQKEVRDSFEDN 241
+E R +N +K+N K + ++ T+ DR +V+ + +A KEVR++ DN
Sbjct: 673 EVEGLANHLRRFYNL-------VKENYLKEVGELTTL-DRWLVSRMQRAIKEVREAM-DN 723
Query: 240 FNTFKTMSA 214
T + ++A
Sbjct: 724 LQTRRAVNA 732
>gi|541386|pir||F53402 cysteine--tRNA ligase (EC 6.1.1.16) - Bacillus stearothermophilus
(fragment)
Length = 221
Score = 45.7 bits (106), Expect = 2e-06
Identities = 17/25 (68%), Positives = 20/25 (80%)
Query: 759 YESKWGEGRPGWHIECSAMAADIFG 685
++S WG+GRPGWHIECSAMA G
Sbjct: 195 WDSPWGKGRPGWHIECSAMAGRYLG 219
Score = 27.0 bits (58), Expect = 2e-06
Identities = 12/22 (54%), Positives = 14/22 (63%)
Query: 799 FVLWKKAKEGEIFL*IKMGRGK 734
F LWK AKEGEI G+G+
Sbjct: 182 FALWKAAKEGEICWDSPWGKGR 203
>gi|135140|sp|P10857|SYLC_NEUCR LEUCYL-TRNA SYNTHETASE, CYTOPLASMIC (LEUCINE--TRNA LIGASE) (LEURS)
>gi|68517|pir||SYNCLC leucine--tRNA ligase (EC 6.1.1.4),
cytosolic - Neurospora crassa >gi|168832 (M30473)
leucyl-tRNA synthetase [Neurospora crassa]
Length = 1123
Score = 52.3 bits (123), Expect = 4e-06
Identities = 42/192 (21%), Positives = 83/192 (42%), Gaps = 1/192 (0%)
Query: 678 LDIHTGAVDLKFPHHENEIHQCQAYFDKEPWVRYFMHSGHLHIDGLKMSKSLKNFITIEE 499
LDI DL H ++ A F +E W + +GHL ++G KMSKS NF+T+++
Sbjct: 711 LDIRVSGKDLIPNHLTFWLYNHIALFPREYWPKSVRANGHLQLNGEKMSKSTGNFMTLDD 770
Query: 498 SLKKYTSRQLRIFFLNTPYNAPMTYKIESMEYAKVQEARIFNFLSVAESKIKDNK-NIFK 322
+KKY + R+ + + +E + A R + E +KD +
Sbjct: 771 VVKKYGADAARVALADAGDGISDSNFVE--DVADNTILRFYTNKEWIEETLKDESLRTGE 828
Query: 321 IMTMEDRMVVNMLIKAQKEVRDSFEDNFNTFKTMSAILNLISVMNQKIDTLTNSTILAVK 142
+ + +D + N + E R +E+ +A + ++ + + + I K
Sbjct: 829 LNSFQDALFDNEMNALVNEARKHYEETSYKLALKAAHYDFLNARDMYREACAAAGIPLHK 888
Query: 141 NFIEKILRILGVI 103
+ + K +R+ ++
Sbjct: 889 DLVTKYIRLQALV 901
>gi|135141|sp|P26637|SYLC_YEAST LEUCYL-TRNA SYNTHETASE, CYTOPLASMIC (LEUCINE--TRNA LIGASE) (LEURS)
>gi|322939|pir||JC1421 leucine--tRNA ligase (EC 6.1.1.4),
cytosolic - yeast (Saccharomyces cerevisiae)
>gi|3508|emb|CAA44671| (X62878) Leucyl-tRNA synthetase
(cytoplasmic) [Saccharomyces cerevisiae]
>gi|1370340|emb|CAA97865| (Z73516) ORF YPL160w
[Saccharomyces cerevisiae] >gi|1403549|emb|CAA65561|
(X96770) P2564 protein [Saccharomyces cerevisiae]
Length = 1090
Score = 48.4 bits (113), Expect = 6e-05
Identities = 35/114 (30%), Positives = 55/114 (47%)
Query: 678 LDIHTGAVDLKFPHHENEIHQCQAYFDKEPWVRYFMHSGHLHIDGLKMSKSLKNFITIEE 499
LD+ DL H I+ A F K+ W + +GHL ++ KMSKS NF+T+E+
Sbjct: 683 LDVSISGKDLIPNHLTFFIYTHVALFPKKFWPKGIRANGHLMLNNSKMSKSTGNFMTLEQ 742
Query: 498 SLKKYTSRQLRIFFLNTPYNAPMTYKIESMEYAKVQEARIFNFLSVAESKIKDN 337
+++K+ + RI F + ES A + R+FN AE K++
Sbjct: 743 TVEKFGADAARIAFADAGDTVEDANFDESNANAAI--LRLFNLKEWAEEITKES 794
>gi|171768 (M19992) isoleucyl-tRNA synthetase (EC 6.1.1.5) [Saccharomyces
cerevisiae]
Length = 1073
Score = 45.7 bits (106), Expect = 4e-04
Identities = 48/164 (29%), Positives = 81/164 (49%), Gaps = 21/164 (12%)
Query: 603 FDKEPWVRYFMHSGH-LHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLNTPYNAPMT 427
F P+ + + SG L DG KMSKSLKN+ L KY + LR++ +N+P
Sbjct: 581 FGSVPYKKSVIVSGIVLAADGRKMSKSLKNYPDPSIVLNKYGADALRLYLINSP-----V 635
Query: 426 YKIESMEYAK--VQEA---------RIFNFLSVAESKIKDNKNI---FKIMTMEDRMVVN 289
K ES+++ + V+E F FL + +K NI + D ++
Sbjct: 636 LKAESLKFKEEGVKEVVSKVLLPWWNSFKFLDGQIALLKKMSNIDFQYDDSVKSDNVMAE 695
Query: 288 MLIKAQKEVRDSFEDNFNTFKTMSAILNLISVMNQKIDTLTNSTI------LAVKNFIEK 127
M++ + + + + +K + + L++ ID LTN I L +N +E
Sbjct: 696 MILASMQSLVQVIHEEMGQYKLYTVVPKLLNF----IDELTNWYIRFNRRRLKGENVVED 751
Query: 126 ILRIL 112
L+ L
Sbjct: 752 CLKAL 756
>gi|1711638|sp|Q10490|SYLC_SCHPO PUTATIVE LEUCYL-TRNA SYNTHETASE, CYTOPLASMIC (LEUCINE--TRNA LIGASE)
(LEURS) >gi|1314182|emb|CAA97370| (Z73100) unknown
[Schizosaccharomyces pombe]
Length = 1111
Score = 45.7 bits (106), Expect = 4e-04
Identities = 25/71 (35%), Positives = 39/71 (54%)
Query: 675 DIHTGAVDLKFPHHENEIHQCQAYFDKEPWVRYFMHSGHLHIDGLKMSKSLKNFITIEES 496
DI DL H ++ A FD++ W + +GHL ++G KMSKS NF+T+ E+
Sbjct: 692 DIRASGKDLVPNHLTFCLYTHTAIFDEKYWPKGIRANGHLLMNGEKMSKSTGNFMTLHEA 751
Query: 495 LKKYTSRQLRI 463
KK+ + R+
Sbjct: 752 TKKFGADATRL 762
>gi|3257379|dbj|BAA30062| (AP000004) 967aa long hypothetical leucyl-tRNA synthetase [Pyrococcus
horikoshii]
Length = 967
Score = 43.0 bits (99), Expect = 0.003
Identities = 41/164 (25%), Positives = 81/164 (49%), Gaps = 3/164 (1%)
Query: 678 LDIHTGAVDLKFPHHENEIHQCQAYFDKEPWVRYFMHSGHLHIDGLKMSKSLKNFITIEE 499
LD DL H I A F +E W + +G ++G KMSKS N + +
Sbjct: 604 LDWRCSGKDLIPNHLTFFIFNHVAIFREEHWPKGIAVNGFGTLEGQKMSKSKGNVLNFID 663
Query: 498 SLKKYTSRQLRIFFLN-TPYNAPMTYKIESMEYAKVQEARIFNFLS-VAESKIKDNKNIF 325
++++ + +R++ ++ +++ ++ + + + Q R + +S AE ++K N +
Sbjct: 664 AIEENGADVVRLYIMSLAEHDSDFDWRRKEVGKLRKQIERFYELISQFAEYEVKGNVELK 723
Query: 324 KIMTMEDRMVVNMLIKAQKEVRDSFEDNFNTFKTMSAI-LNLISVMN 187
I DR +++ L KA KE ++ E+ F+T +A+ S+MN
Sbjct: 724 DI----DRWMLHRLNKAIKETTNALEE----FRTRTAVQWAFYSIMN 762
>gi|2129086|pir||A64379 isoleucine--tRNA ligase (EC 6.1.1.5) - Methanococcus jannaschii
Length = 942
Score = 43.0 bits (99), Expect = 0.003
Identities = 51/210 (24%), Positives = 98/210 (46%), Gaps = 3/210 (1%)
Query: 678 LDIHTGAVDLKFPHHENEIHQCQAYFDKEPWVRYFMHSGHLHIDGLKMSKSLKNFITIEE 499
+D A DL H I A F +E W R + +G++ I+G K+SKS + + E
Sbjct: 587 VDWRCSAKDLIPNHLTFYIFNHVAIFPEEFWPRGIVVNGYVTIEGKKLSKSKGPVLPVLE 646
Query: 498 SLKKYTSRQLRIFFLNTPYNAPM--TYKIESMEYAKVQEARIFNFL-SVAESKIKDNKNI 328
+K+ + R F++ T P K + ME K R++ F +AE + + +
Sbjct: 647 VAEKFGADVGR-FYITTCAELPQDADIKFKEMENTKKVLERLYLFAKEIAERRGETGEEF 705
Query: 327 FKIMTMEDRMVVNMLIKAQKEVRDSFEDNFNTFKTMSAILNLISVMNQKIDTLTNSTILA 148
I D+ +++ L KA K+ D + +NF K + L+ + + + I
Sbjct: 706 SYI----DKWLLSRLYKAVKQY-DEYMENFELRKAGILLYQLLDDL-KWYRRRGGNNIRV 759
Query: 147 VKNFIEKILRILGVITEEVNKTEDNDVLDILGE 49
++ F+E I++++ T + + ++ +ILG+
Sbjct: 760 LEEFLEVIIKLMMPFTPHLCE----EMWEILGK 788
>gi|3915868|sp|Q58050|SYL_METJA LEUCYL-TRNA SYNTHETASE (LEUCINE--TRNA LIGASE) (LEURS) >gi|2826301
(U67511) leucyl-tRNA synthetase (leuS) [Methanococcus
jannaschii]
Length = 942
Score = 43.0 bits (99), Expect = 0.003
Identities = 51/210 (24%), Positives = 98/210 (46%), Gaps = 3/210 (1%)
Query: 678 LDIHTGAVDLKFPHHENEIHQCQAYFDKEPWVRYFMHSGHLHIDGLKMSKSLKNFITIEE 499
+D A DL H I A F +E W R + +G++ I+G K+SKS + + E
Sbjct: 587 VDWRCSAKDLIPNHLTFYIFNHVAIFPEEFWPRGIVVNGYVTIEGKKLSKSKGPVLPVLE 646
Query: 498 SLKKYTSRQLRIFFLNTPYNAPM--TYKIESMEYAKVQEARIFNFL-SVAESKIKDNKNI 328
+K+ + R F++ T P K + ME K R++ F +AE + + +
Sbjct: 647 VAEKFGADVGR-FYITTCAELPQDADIKFKEMENTKKVLERLYLFAKEIAERRGETGEEF 705
Query: 327 FKIMTMEDRMVVNMLIKAQKEVRDSFEDNFNTFKTMSAILNLISVMNQKIDTLTNSTILA 148
I D+ +++ L KA K+ D + +NF K + L+ + + + I
Sbjct: 706 SYI----DKWLLSRLYKAVKQY-DEYMENFELRKAGILLYQLLDDL-KWYRRRGGNNIRV 759
Query: 147 VKNFIEKILRILGVITEEVNKTEDNDVLDILGE 49
++ F+E I++++ T + + ++ +ILG+
Sbjct: 760 LEEFLEVIIKLMMPFTPHLCE----EMWEILGK 788
>gi|628472|pir||C53739 vacA 5'-region protein - Helicobacter pylori (ATCC 49503)
(fragment) >gi|471728 (U05676) cysteinyl-tRNA synthetase
homolog [Helicobacter pylori]
Length = 188
Score = 42.2 bits (97), Expect = 0.004
Identities = 39/168 (23%), Positives = 78/168 (46%), Gaps = 13/168 (7%)
Query: 507 IEESLKKYTSRQLRIFFLNTPYNAPMTYKIESMEYAKVQEARIFNFLSVAESKIKDNKNI 328
I+++LK Y LR + L Y + + + E + +K K +
Sbjct: 3 IKDALKNYDGEILRNYLLGVHYRSVLNFNEEDLLVSK--------------------KRL 42
Query: 327 FKIMTMEDRMVVNM---LIKAQKEVRDSFEDNFNTFKTMSAILNLISVMNQKID-----T 172
KI ++ R++ N+ +KE+ + +D+ N K +S + +++S N+K+D
Sbjct: 43 DKIYRLKQRVLGNLGGINPNFKKEILECMQDDLNVSKALSVLESMLSSTNEKLDQNPKNK 102
Query: 171 LTNSTILAVKNFIEKILRI-----LGVITEEVNKTEDNDVLDILGEYRNAIRKLAAKKKD 7
ILA F+E++L I V+++E ++ + + E + A + K D
Sbjct: 103 ALKGEILANLKFVEELLGIGFKDPSAYFQLGVSESEKQEIENKIEERKRAKEQKDFLKAD 162
Query: 6 H 4
H
Sbjct: 163 H 163
>gi|135134|sp|P09436|SYIC_YEAST ISOLEUCYL-TRNA SYNTHETASE, CYTOPLASMIC (ISOLEUCINE--TRNA LIGASE)
(ILERS) >gi|68524|pir||SYBYI4 isoleucine--tRNA ligase (EC
6.1.1.5) - yeast (Saccharomyces cerevisiae)
>gi|3820|emb|CAA30733| (X07886) ILSI (AA 1-1072)
[Saccharomyces cerevisiae] >gi|498991|emb|CAA56034|
(X79489) ILS1 protein [Saccharomyces cerevisiae]
>gi|536123|emb|CAA84898| (Z35838) ORF YBL076c
[Saccharomyces cerevisiae]
Length = 1072
Score = 41.4 bits (95), Expect = 0.007
Identities = 45/164 (27%), Positives = 77/164 (46%), Gaps = 20/164 (12%)
Query: 603 FDKEPWVRYFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLNTPYNAPMTY 424
F P+ + L DG KMSKSLKN+ L KY + LR++ +N+P
Sbjct: 581 FGSVPYKNVIVSGIVLAADGRKMSKSLKNYPDPSIVLNKYGADALRLYLINSP-----VL 635
Query: 423 KIESMEYAK--VQEA---------RIFNFLSVAESKIKDNKNI---FKIMTMEDRMVVNM 286
K ES+++ + V+E F FL + +K NI + D ++
Sbjct: 636 KAESLKFKEEGVKEVVSKVLLPWWNSFKFLDGQIALLKKMSNIDFQYDDSVKSDNVMDRW 695
Query: 285 LIKAQKEVRDSFEDNFNTFKTMSAILNLISVMNQKIDTLTNSTI------LAVKNFIEKI 124
++ + + + + +K + + L++ ID LTN I L +N +E
Sbjct: 696 ILASMQSLVQFIHEEMGQYKLYTVVPKLLNF----IDELTNWYIRFNRRRLKGENGVEDC 751
Query: 123 LRIL 112
L+ L
Sbjct: 752 LKAL 755
>gi|1711637|sp|Q09996|SYLC_CAEEL PROBABLE LEUCYL-TRNA SYNTHETASE (LEUCINE--TRNA LIGASE) (LEURS)
Length = 1198
Score = 41.0 bits (94), Expect = 0.010
Identities = 17/46 (36%), Positives = 31/46 (66%)
Query: 600 DKEPWVRYFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRI 463
D W + +GHL ++ KMSKS NF+T+EE+++K+++ +R+
Sbjct: 710 DTSKWPKGIRANGHLLLNNEKMSKSTGNFMTLEEAIEKFSADGMRL 755
>gi|3874391|emb|CAA86751| (Z46787) similar to leucyl-tRNA synthetase; cDNA EST EMBL:D64208
comes from this gene; cDNA EST EMBL:D65056 comes from
this gene; cDNA EST EMBL:D68348 comes from this gene;
cDNA EST yk377h12.3 comes from this gene; cDNA EST
yk3... >gi|3879166|emb|CAA85280| (Z36238) similar to
leucyl-tRNA synthetase; cDNA EST EMBL:D64208 comes from
this gene; cDNA EST EMBL:D65056 comes from this gene;
cDNA EST EMBL:D68348 comes from this gene; cDNA EST
yk377h12.3 comes from this gene; cDNA EST yk3...
Length = 1186
Score = 41.0 bits (94), Expect = 0.010
Identities = 17/46 (36%), Positives = 31/46 (66%)
Query: 600 DKEPWVRYFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRI 463
D W + +GHL ++ KMSKS NF+T+EE+++K+++ +R+
Sbjct: 698 DTSKWPKGIRANGHLLLNNEKMSKSTGNFMTLEEAIEKFSADGMRL 743
>gi|135128|sp|P26499|SYI_METTM ISOLEUCYL-TRNA SYNTHETASE (ISOLEUCINE--TRNA LIGASE) (ILERS)
Length = 1046
Score = 39.9 bits (91), Expect = 0.022
Identities = 22/53 (41%), Positives = 32/53 (59%), Gaps = 2/53 (3%)
Query: 600 DKEPWVRYFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFL--NTPY 442
D+ P+ R MH L +G KMSKSL N + E+ ++KY + LR + L N P+
Sbjct: 572 DETPYRRVLMHGFTLDEEGRKMSKSLGNVVEPEDVIEKYGADVLRFYLLWANKPW 626
>gi|68525|pir||SYEXI isoleucine--tRNA ligase (EC 6.1.1.5) - Methanobacterium
thermoautotrophicum >gi|149728 (M59245) transfer RNA-Ile
synthetase [Methanobacterium thermoautotrophicum]
Length = 1045
Score = 39.9 bits (91), Expect = 0.022
Identities = 22/53 (41%), Positives = 32/53 (59%), Gaps = 2/53 (3%)
Query: 600 DKEPWVRYFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFL--NTPY 442
D+ P+ R MH L +G KMSKSL N + E+ ++KY + LR + L N P+
Sbjct: 571 DETPYRRVLMHGFTLDEEGRKMSKSLGNVVEPEDVIEKYGADVLRFYLLWANKPW 625
>gi|3122898|sp|O29622|SYI_ARCFU ISOLEUCYL-TRNA SYNTHETASE (ISOLEUCINE--TRNA LIGASE) (ILERS)
>gi|2649988 (AE001061) isoleucyl-tRNA synthetase (ileS)
[Archaeoglobus fulgidus]
Length = 1018
Score = 39.5 bits (90), Expect = 0.029
Identities = 35/119 (29%), Positives = 59/119 (49%), Gaps = 10/119 (8%)
Query: 603 FDKEPWVRYFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLNTPYNAPMTY 424
FDK P+ MH L G KMSKSL N + EE + + R++ L Y+AP
Sbjct: 565 FDKAPYKAVLMHGFTLDEQGRKMSKSLGNVVEPEEVVGQIGVDGFRLYVL---YSAPW-- 619
Query: 423 KIESMEYAKVQEARIFNFLSVAESKIK--------DNKNIFK--IMTMEDRMVVNMLIKA 274
E + ++ + I L+V + ++ DN + + + +EDR +++ L
Sbjct: 620 --EDLRFSWEEARNINRMLNVVWNAVRFAHTYMSLDNYSFGEKGELKIEDRWILSRLESF 677
Query: 273 QKEVRDSFE 247
KE ++ E
Sbjct: 678 IKEANEAME 686
>gi|3334357|sp|O27428|SYI_METTH ISOLEUCYL-TRNA SYNTHETASE (ISOLEUCINE--TRNA LIGASE) (ILERS)
>gi|2622484 (AE000900) isoleucyl-tRNA synthetase
[Methanobacterium thermoautotrophicum]
Length = 1044
Score = 39.5 bits (90), Expect = 0.029
Identities = 22/53 (41%), Positives = 32/53 (59%), Gaps = 2/53 (3%)
Query: 600 DKEPWVRYFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFL--NTPY 442
D+ P+ R MH L +G KMSKSL N + E+ ++KY + LR + L N P+
Sbjct: 571 DEVPYRRVLMHGFTLDEEGRKMSKSLGNVVEPEDVIEKYGADVLRFYLLWENKPW 625
>gi|3122901|sp|O13651|SYIC_SCHPO ISOLEUCYL-TRNA SYNTHETASE, CYTOPLASMIC (ISOLEUCINE--TRNA LIGASE)
(ILERS) >gi|2257546|dbj|BAA21439| (AB004538)
ISOLEUCYL-TRNA SYNTHETASE ,CYTOPLASMIC
[Schizosaccharomyces pombe] >gi|2950493|emb|CAA17821|
(AL022072) isoleucyl-trna synthetase, cytoplasmic
[Schizosaccharomyces pombe]
Length = 1064
Score = 39.5 bits (90), Expect = 0.029
Identities = 20/53 (37%), Positives = 30/53 (55%)
Query: 603 FDKEPWVRYFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLNTP 445
FDK P+ + + DG KMSK LKN+ ++KY S LR++ +N+P
Sbjct: 576 FDKAPYKNVIVSGLVMAEDGKKMSKRLKNYPEPNLIIEKYGSDALRLYLINSP 628
>gi|2984068 (AE000755) leucyl-tRNA synthetase beta subunit [Aquifex aeolicus]
Length = 289
Score = 39.1 bits (89), Expect = 0.038
Identities = 41/166 (24%), Positives = 77/166 (45%), Gaps = 8/166 (4%)
Query: 549 DGLKMSKSLKNFITIEESLKKYTSRQLRIFFL-NTPYNAPMTYKIESMEYAKVQEARIFN 373
+ KMSKS N + EE+++KY + +R++ L P + E ++ A R +N
Sbjct: 42 ESAKMSKSKANVVDPEEAVEKYGADTVRLYILFAAPPEQDFEWTDEGIQGAYRFLQRYWN 101
Query: 372 FLSVAESKIKDNKNIFKIMTMEDRMVVNMLIKAQKEV-------RDSFEDNFNTFKTMSA 214
F++ KIK+ E R V + ++E+ R FE+ + ++
Sbjct: 102 FVNKHLEKIKN----LTYTVEELRNVQGKAKEVRREIHQTIADYRRDFEERYQFNTAIAK 157
Query: 213 ILNLISVMNQKIDTLTNSTILAVKNFIEKILRILGVITEEVNKTEDNDVLDILG 52
I+ L++ + Q T ++ IE I +L IT + + +V ++LG
Sbjct: 158 IMKLLNTL-QDFSPQTEQDYKVLREGIETITLLLSPITPHIAE----EVWEMLG 206
>gi|3122906|sp|O26861|SYV_METTH VALYL-TRNA SYNTHETASE (VALINE--TRNA LIGASE) (VALRS) >gi|2621857
(AE000855) valyl-tRNA synthetase [Methanobacterium
thermoautotrophicum]
Length = 897
Score = 38.7 bits (88), Expect = 0.049
Identities = 30/127 (23%), Positives = 59/127 (45%)
Query: 624 IHQCQAYFDKEPWVRYFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLNTP 445
I +C A ++P+ ++ DG KMSKS N I EE L+ Y + LR++ +
Sbjct: 521 ILRCMALTGEKPFSEIVINGMVFGEDGHKMSKSRGNVIAPEEVLEDYGADALRLWAAGSV 580
Query: 444 YNAPMTYKIESMEYAKVQEARIFNFLSVAESKIKDNKNIFKIMTMEDRMVVNMLIKAQKE 265
+ + + + ++Y + +N + +N + + M DR +++ L+ E
Sbjct: 581 PGSDVPFAWKDVKYGYKFLRKFWNAFRFISIHLTENPEV-EARPM-DRWILSRLMNLVAE 638
Query: 264 VRDSFED 244
V +S +D
Sbjct: 639 VTESLDD 645
>gi|1174531|sp|P43827|SYL_HAEIN LEUCYL-TRNA SYNTHETASE (LEUCINE--TRNA LIGASE) (LEURS)
>gi|1074962|pir||H64102 leucine--tRNA ligase (EC 6.1.1.4)
- Haemophilus influenzae (strain Rd KW20) >gi|1573943
(U32774) leucyl-tRNA synthetase (leuS) [Haemophilus
influenzae Rd]
Length = 861
Score = 38.3 bits (87), Expect = 0.064
Identities = 38/168 (22%), Positives = 81/168 (47%), Gaps = 4/168 (2%)
Query: 594 EPWVRYFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFL-NTPYNAPMTYKI 418
+P R +HSG KMSKS N I +E ++KY + +R+F + +P + ++
Sbjct: 606 DPEGRELVHSGMT-----KMSKSKNNGIDPQEMVEKYGADTVRLFMMFASPAEMTLEWQE 660
Query: 417 ESMEYAKVQEARIFNFLSVAESKIKDNKNIFKIMTMEDRMVVNMLIKAQKEVRDSF--ED 244
+E AK R++N + + ++ E +++ + K +V D
Sbjct: 661 SGVEGAKRFLGRVWNLVYQYQQNPAKTSLDLTALSAEQKVLRREVHKTIAKVSDDIGRRQ 720
Query: 243 NFNTFKTMSAILNLISVMNQ-KIDTLTNSTILAVKNFIEKILRILGVITEEV 91
FNT ++A++ L++ + + +D+ + ++A + ++R+L IT +
Sbjct: 721 TFNT--AIAAVMELMNKLTKASLDSEQDRAVMA--EALSAVVRMLYPITPHI 768
>gi|2983002 (AE000683) isoleucyl-tRNA synthetase [Aquifex aeolicus]
Length = 956
Score = 37.9 bits (86), Expect = 0.084
Identities = 31/131 (23%), Positives = 65/131 (48%), Gaps = 5/131 (3%)
Query: 591 PWVRYFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLNTPYNAPM-----T 427
P+ H + G KMSKSL N I+ +E +K++ + LR++ ++ Y +
Sbjct: 607 PYKAVLTHGFIVDEKGRKMSKSLGNVISPQEVVKEFGADILRLWVVSEDYTEDVKLGKNL 666
Query: 426 YKIESMEYAKVQEARIFNFLSVAESKIKDNKNIFKIMTMEDRMVVNMLIKAQKEVRDSFE 247
K + +Y K++ F ++ + + N F+ + DR +++ L K+V +++E
Sbjct: 667 LKKIADDYRKIRNTLRFIIGNLYDFNPRTNALPFEKLHHFDRWIISELQNLLKKVHENYE 726
Query: 246 DNFNTFKTMSAILNLI 199
F ++ + I N +
Sbjct: 727 -KFLFYRVHNHIKNFV 741
>gi|2501013|sp|Q21926|SYI_CAEEL PROBABLE ISOLEUCYL-TRNA SYNTHETASE, CYTOPLASMIC (ISOLEUCINE--TRNA
LIGASE) (ILERS) >gi|3879120|emb|CAA94369| (Z70310)
predicted using Genefinder; Similarity to Human
isoleucyl-tRNA synthetase (SW:SYI_HUMAN); cDNA EST
EMBL:D33089 comes from this gene; cDNA EST EMBL:D33388
comes from this gene; cDNA EST EMBL:D34131 comes from
this gene;...
Length = 1141
Score = 37.9 bits (86), Expect = 0.084
Identities = 39/171 (22%), Positives = 76/171 (43%), Gaps = 2/171 (1%)
Query: 609 AYFDKEPWVRYFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLNTPYNAPM 430
A F+K P+ + L DG KMSKS KN+ + KY + LR++ +N+P
Sbjct: 578 ALFNKPPFKNLICNGLVLASDGAKMSKSKKNYPDPMLIVNKYGADALRLYLINSP----- 632
Query: 429 TYKIESMEYAKVQEARIFNFLSVAESKIKDNKNIFKIMTMEDRMVVNMLIKAQKEVRDSF 250
+ E + +E + + L K++F R V + + E + F
Sbjct: 633 ---VVRGENLRFREEGVRDLL----------KDVFLPWFNAYRFFVQNVQAYEHETGNVF 679
Query: 249 EDNFNTFKTMSAILNLISVMNQKIDTLTNSTILAVKNFIE--KILRILGVITE 97
+ N + +VM++ I++ TNS + V+ ++ ++ ++G +T+
Sbjct: 680 DMNVHVASE--------NVMDRWIESFTNSLVAFVRKEMDSYRLYAVVGPLTK 724
>gi|2501065|sp|P56000|SYV_HELPY VALYL-TRNA SYNTHETASE (VALINE--TRNA LIGASE) (VALRS)
>gi|2314306|gb|AAD08195.1| (AE000621) valyl-tRNA
synthetase (valS) [Helicobacter pylori 26695]
Length = 874
Score = 37.9 bits (86), Expect = 0.084
Identities = 45/184 (24%), Positives = 79/184 (42%), Gaps = 9/184 (4%)
Query: 771 ERYFYESKWGEGRPGWHIECSAM--AADI--FGDELDIHTGAVDLKFPHHENEIHQCQAY 604
+ +F W GW E S + +D+ F + TG D+ F + ++
Sbjct: 453 DTWFSSGLWAFSTLGWGQEKSGLFNESDLKDFYPNTTLITG-FDILFFWVARMLFCSESL 511
Query: 603 FDKEPWVRYFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLN-TPYNAPMT 427
+ P+ ++H+ G KMSKS N I E ++KY + LR N +
Sbjct: 512 LGELPFKDIYLHALVRDEKGEKMSKSKGNVIDPLEMIEKYGADSLRFTLANLCATGRDIK 571
Query: 426 YKIESMEYAKVQEARIFN---FLSVAESKIKDNKNIFKIMTMEDRMVVNMLIKAQKEVRD 256
+E K ++FN +L + + KD + + + T R + L A KE R+
Sbjct: 572 LSTTHLENNKNFANKLFNAASYLKLKQESFKDKERLNEYQTPLGRYAKSRLNSATKEARN 631
Query: 255 SFED-NFNTFKTM 220
+ ++ FN T+
Sbjct: 632 ALDNYRFNDATTL 644
>gi|2160156 (AC000132) Strong similarity to S. pombe leucyl-tRNA synthetase
(gb|Z73100). [Arabidopsis thaliana]
Length = 1084
Score = 37.5 bits (85), Expect = 0.11
Identities = 21/71 (29%), Positives = 35/71 (48%)
Query: 678 LDIHTGAVDLKFPHHENEIHQCQAYFDKEPWVRYFMHSGHLHIDGLKMSKSLKNFITIEE 499
LD+ DL H I+ A W R +GH+ ++ KMSKS NF T+ +
Sbjct: 669 LDLRVSGKDLIQNHLTFFIYNHTALMANRNWPRGIRCNGHIMLNSEKMSKSTGNFRTLRQ 728
Query: 498 SLKKYTSRQLR 466
S++++++ R
Sbjct: 729 SIEEFSATGTR 739
>gi|2501068|sp|Q58413|SYV_METJA VALYL-TRNA SYNTHETASE (VALINE--TRNA LIGASE) (VALRS)
>gi|2129352|pir||F64425 valine--tRNA ligase (EC 6.1.1.9)
- Methanococcus jannaschii >gi|1591666 (U67543)
valyl-tRNA synthetase (valS) [Methanococcus jannaschii]
Length = 878
Score = 37.1 bits (84), Expect = 0.14
Identities = 31/122 (25%), Positives = 58/122 (47%), Gaps = 6/122 (4%)
Query: 597 KEPWVRYFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLNTPYNAPMTYKI 418
K+PW ++ DG KMSKS N + +E + KY + LR++ N+ + +
Sbjct: 508 KKPWDEIVINGMVFGEDGHKMSKSRGNVVEPDEIIAKYGADALRLWASNSVVGDDVQFLW 567
Query: 417 ESMEYA-----KVQEARIFNFLSVAESKIKDNKNIFKIMTMEDRMVVNMLIKAQKEVRDS 253
+ ++Y K A F + +++ I + K +I D +++ L + + V
Sbjct: 568 KEVDYGYRFLRKSWNACRFAKMHISDDIIDELKKPMEISNPIDLWILSKLQRLIERVDKD 627
Query: 252 FED-NFNT 232
E+ FNT
Sbjct: 628 LENYRFNT 635
>gi|3861224|emb|CAA15124| (AJ235272) VALYL-TRNA SYNTHETASE (valS) [Rickettsia prowazekii]
Length = 814
Score = 36.7 bits (83), Expect = 0.19
Identities = 32/116 (27%), Positives = 60/116 (51%), Gaps = 10/116 (8%)
Query: 591 PWVRYFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLNTPYNAPMTYKIES 412
PW + L D KMSKS N + E+ L++Y + +R + N+ A Y +
Sbjct: 519 PWKNIMVSGWCLAEDRSKMSKSKGNVLVPEKLLERYGADVIRYWSANSKLGADTAYSEDV 578
Query: 411 MEYAKVQEARIFN---FLSVAESKI--KDNK-NIF----KIMTMEDRMVVNMLIKAQKEV 262
M+ K +++N F+S+ K+ +D K ++F KI D+ ++N L+ K
Sbjct: 579 MKNGKRLVNKLWNAAKFVSIHFDKLTSEDKKVSLFDIKEKITNEFDQWMINKLVALVKLA 638
Query: 261 RDSFED 244
++ ++
Sbjct: 639 TNALQN 644
>gi|4155674 (AE001535) VALYL-TRNA SYNTHETASE [Helicobacter pylori J99]
Length = 872
Score = 36.7 bits (83), Expect = 0.19
Identities = 45/184 (24%), Positives = 79/184 (42%), Gaps = 9/184 (4%)
Query: 771 ERYFYESKWGEGRPGWHIECSAM--AADI--FGDELDIHTGAVDLKFPHHENEIHQCQAY 604
+ +F W GW E S + +D+ F + TG D+ F + ++
Sbjct: 451 DTWFSSGLWAFSTLGWGQEKSDLFNESDLKDFYPNTTLITG-FDILFFWVARMLFCSESL 509
Query: 603 FDKEPWVRYFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLN-TPYNAPMT 427
+ P+ ++H+ G KMSKS N I E ++KY + LR N +
Sbjct: 510 LGELPFKDIYLHALVRDEKGEKMSKSKGNVIDPLEMIEKYGADSLRFTLANLCATGRDIK 569
Query: 426 YKIESMEYAKVQEARIFN---FLSVAESKIKDNKNIFKIMTMEDRMVVNMLIKAQKEVRD 256
+E K ++FN +L + + KD + + + T R + L KEVR+
Sbjct: 570 LSTTHLENNKNFANKLFNAASYLKLKQESFKDKERLNEYQTALGRYAKSRLNLVTKEVRN 629
Query: 255 SFED-NFNTFKTM 220
+ ++ FN T+
Sbjct: 630 ALDNYRFNDATTL 642
>gi|4504555|oth|NP_002152|PIARS isoleucine-tRNA synthetase >gi|440799 (U04953) isoleucyl-tRNA
synthetase [Homo sapiens]
Length = 1262
Score = 36.0 bits (81), Expect = 0.33
Identities = 36/170 (21%), Positives = 73/170 (42%), Gaps = 3/170 (1%)
Query: 609 AYFDKEPWVRYFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLNTPYNAPM 430
A F + P+ ++ L DG KMSK KN+ ++KY + LR++ +N+P
Sbjct: 577 ALFGQPPFKNVIVNGLVLASDGQKMSKRKKNYPDPVSIIQKYGADALRLYLINSP----- 631
Query: 429 TYKIESMEYAKVQEARIFNFLSVAESKIKDNKNIFKIMTMEDRMVVNMLIKAQKEVRDSF 250
+ E + +E + + L K++ R ++ +++ QKE F
Sbjct: 632 ---VVRAENLRFKEEGVRDVL----------KDVLLPWYNAYRFLIQNVLRLQKEEEIEF 678
Query: 249 EDNFNTFKTMSAILN--LISVMNQKIDTL-TNSTILAVKNFIEKILRILGVIT 100
N NT + I + ++S M I T + + ++++ + ++T
Sbjct: 679 LYNENTVRESPNITDRWILSFMQSLIGFFETEMAAYRLYTVVPRLVKFVDILT 731
>gi|2501012|sp|Q58357|SYI_METJA ISOLEUCYL-TRNA SYNTHETASE (ISOLEUCINE--TRNA LIGASE) (ILERS)
>gi|2129087|pir||C64418 isoleucine--tRNA ligase (EC
6.1.1.5) - Methanococcus jannaschii >gi|1591614 (U67538)
isoleucyl-tRNA synthetase (ileS) [Methanococcus
jannaschii]
Length = 1039
Score = 36.0 bits (81), Expect = 0.33
Identities = 29/119 (24%), Positives = 56/119 (46%), Gaps = 6/119 (5%)
Query: 603 FDKEPWVRYFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLN-TPYNAPMT 427
F+ P+ + MH L G KMSKSL N + ++ ++KY + LR + L+ +
Sbjct: 579 FNDIPYKKCLMHGFTLDEHGDKMSKSLGNVVNPDDVVEKYGADLLRFYLLSANKVWEDLR 638
Query: 426 YKIESME-----YAKVQEARIFNFLSVAESKIKDNKNIFKIMTMEDRMVVNMLIKAQKEV 262
+ M+ + + A +F + K ++ F+ + EDR +V+ + K
Sbjct: 639 FVWSEMDDVLSLFNTLWNAYMFAVNYMVLDNFKPDEKYFEYLKDEDRWIVSRINSVAKIA 698
Query: 261 RDSFE 247
++ E
Sbjct: 699 IENLE 703
>gi|730870|sp|P41252|SYI_HUMAN ISOLEUCYL-TRNA SYNTHETASE, CYTOPLASMIC (ISOLEUCINE--TRNA LIGASE)
(ILERS) >gi|2135522|pir||I59314 Isoleucyl tRNA Synthetase
- human >gi|551622|dbj|BAA05835| (D28473) isoleucyl-tRNA
synthetase [Homo sapiens]
Length = 1266
Score = 36.0 bits (81), Expect = 0.33
Identities = 36/170 (21%), Positives = 73/170 (42%), Gaps = 3/170 (1%)
Query: 609 AYFDKEPWVRYFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLNTPYNAPM 430
A F + P+ ++ L DG KMSK KN+ ++KY + LR++ +N+P
Sbjct: 581 ALFGQPPFKNVIVNGLVLASDGQKMSKRKKNYPDPVSIIQKYGADALRLYLINSP----- 635
Query: 429 TYKIESMEYAKVQEARIFNFLSVAESKIKDNKNIFKIMTMEDRMVVNMLIKAQKEVRDSF 250
+ E + +E + + L K++ R ++ +++ QKE F
Sbjct: 636 ---VVRAENLRFKEEGVRDVL----------KDVLLPWYNAYRFLIQNVLRLQKEEEIEF 682
Query: 249 EDNFNTFKTMSAILN--LISVMNQKIDTL-TNSTILAVKNFIEKILRILGVIT 100
N NT + I + ++S M I T + + ++++ + ++T
Sbjct: 683 LYNENTVRESPNITDRWILSFMQSLIGFFETEMAAYRLYTVVPRLVKFVDILT 735
>gi|2688775 (AE001181) isoleucyl-tRNA synthetase (ileS) [Borrelia burgdorferi]
Length = 1042
Score = 34.8 bits (78), Expect = 0.73
Identities = 32/141 (22%), Positives = 67/141 (46%), Gaps = 14/141 (9%)
Query: 609 AYFDKEPWVRYFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLNTPYNAPM 430
A F+ + ++ L DG KMSKS KN+ + + + + LR++ + +P
Sbjct: 571 ALFENTAFKNVIVNGLVLSSDGRKMSKSFKNYTDPMQVINTFGADALRLYLIMSP----- 625
Query: 429 TYKIESMEYA-------------KVQEARIFNFLSVAESKIKDNKNIFKIMTME-DRMVV 292
K + ++Y+ + A F K K KN+ D+ ++
Sbjct: 626 VVKADDLKYSDNGVRDVLKNIIIPIWNAYSFFTTYAIIDKFKPPKNLSLAKNNNLDKWII 685
Query: 291 NMLIKAQKEVRDSFEDNFNTFKTMSAILNLISVMN 187
+ L ++ K++ ++ D +N K++ ++L I +N
Sbjct: 686 SEL-ESLKKILNTEIDKYNLTKSIESLLEFIDKLN 719
>gi|4156066 (AE001567) LEUCYL-TRNA SYNTHETASE [Helicobacter pylori J99]
Length = 806
Score = 34.4 bits (77), Expect = 0.96
Identities = 19/45 (42%), Positives = 28/45 (62%)
Query: 549 DGLKMSKSLKNFITIEESLKKYTSRQLRIFFLNTPYNAPMTYKIE 415
DG KMSKS N ++ +E LKKY + +R+F L + AP ++E
Sbjct: 569 DGAKMSKSKGNVVSPKEILKKYGADAVRLFIL---FAAPPAKELE 610
>gi|3122908|sp|O27552|SYL_METTH LEUCYL-TRNA SYNTHETASE (LEUCINE--TRNA LIGASE) (LEURS) >gi|2622626
(AE000911) leucyl-tRNA synthetase [Methanobacterium
thermoautotrophicum]
Length = 937
Score = 34.4 bits (77), Expect = 0.96
Identities = 22/77 (28%), Positives = 38/77 (48%)
Query: 678 LDIHTGAVDLKFPHHENEIHQCQAYFDKEPWVRYFMHSGHLHIDGLKMSKSLKNFITIEE 499
LD A DL H I A F + W R + G ++G KMS S N I + +
Sbjct: 563 LDWRLSAKDLIGNHLTFHIFHHSAIFPESGWPRGAVVFGMGLLEGNKMSSSKGNVILLRD 622
Query: 498 SLKKYTSRQLRIFFLNT 448
+++K+ + +R+F +++
Sbjct: 623 AIEKHGADVVRLFLMSS 639
>gi|1174521|sp|P41972|SYI_STAAU ISOLEUCYL-TRNA SYNTHETASE (ISOLEUCINE--TRNA LIGASE) (ILERS)
>gi|541337|pir||S40178 isoleucine--tRNA ligase (EC
6.1.1.5) - Staphylococcus aureus
>gi|437916|emb|CAA52296| (X74219) isoleucyl-tRNA
synthetase [Staphylococcus aureus]
Length = 917
Score = 34.4 bits (77), Expect = 0.96
Identities = 35/133 (26%), Positives = 60/133 (44%), Gaps = 5/133 (3%)
Query: 591 PWVRYFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLNTPYNAPMTYKIES 412
P+ H + +G KMSKSL N I ++ +K+ + R++ +T Y A + E
Sbjct: 578 PYKFLLSHGFVMDGEGKKMSKSLGNVIVPDQVVKQKGADIARLWVSSTDYLADVRISDEI 637
Query: 411 MEYAKVQEARIFNFLSVAESKIKD-NKNIFKIMTME----DRMVVNMLIKAQKEVRDSFE 247
++ +I N L I D N + I E DR ++N L + +++E
Sbjct: 638 LKQTSDVYRKIRNTLRFMLGNINDFNPDTDSIPESELLEVDRYLLNRLREFTASTINNYE 697
Query: 246 DNFNTFKTMSAILNLISV 193
NF+ + N I+V
Sbjct: 698 -NFDYLNIYQEVQNFINV 714
>gi|2293014|emb|CAA71688| (Y10687) purine NTPase [Sulfolobus acidocaldarius]
Length = 886
Score = 34.0 bits (76), Expect = 1.3
Identities = 41/198 (20%), Positives = 83/198 (41%), Gaps = 4/198 (2%)
Query: 684 DELDIHTGAVDLKFPHHENEIHQCQAYFD--KEPWVRYFMHSGHLHIDGLKMSKSLKNFI 511
+E+ + ++ + E+E++Q F+ KE V+Y + SG L + K+ +
Sbjct: 206 EEIKVKLENIEREAKEKEDELNQYNTEFNRIKEIKVQYDILSGELSVVNKKIEEIALRLK 265
Query: 510 TIEESLKKYTSRQLRIFFLNTPYNAPMTYKIESMEYAKVQEARIFNFLSVAESKIKDNKN 331
EE K+Y + + L+ N I S + VQ + + ++V E+ +K K
Sbjct: 266 DFEEKEKRYNKIETEVKELDE--NREKINTISSFKSILVQIDSLKSQINVVENDLKRKKE 323
Query: 330 IFKIM--TMEDRMVVNMLIKAQKEVRDSFEDNFNTFKTMSAILNLISVMNQKIDTLTNST 157
K E + K +KE+ + + K ++ +L I +I+ L
Sbjct: 324 KLKRKKELEEKEKQYEEIEKRKKELEEKEKQYEEIEKRLTYVLKNIERQKNEIEKLNYVD 383
Query: 156 ILAVKNFIEKILRILGVITEEV 91
++N I+ + + I E+
Sbjct: 384 TQDLENKIKDVSDRINQIDNEL 405
Score = 31.7 bits (70), Expect = 6.3
Identities = 28/137 (20%), Positives = 68/137 (49%), Gaps = 20/137 (14%)
Query: 423 KIESMEYAKVQEARIFNFLSVAESKIKDNKNIFKIMTMEDRMV----------------V 292
KI +E + I + + V +I+ ++I I+ E+ + +
Sbjct: 156 KINKLEMLRESNGPIHSLIKVLTDRIRSLQSIKDILKREEAEIDRLKKEIEEIKVKLENI 215
Query: 291 NMLIKAQKEVRDSFEDNFNTFKTMSAILNLIS----VMNQKIDTLTNSTILAVKNFIEKI 124
K +++ + + FN K + +++S V+N+KI+ + L +K+F EK
Sbjct: 216 EREAKEKEDELNQYNTEFNRIKEIKVQYDILSGELSVVNKKIEEIA----LRLKDFEEKE 271
Query: 123 LRILGVITEEVNKTEDNDVLDILGEYRNAIRKLAAKK 13
R + TE E+ + ++ + +++ + ++ + K
Sbjct: 272 KRYNKIETEVKELDENREKINTISSFKSILVQIDSLK 308
>gi|2447060 (U42580) A559L [Paramecium bursaria Chlorella virus 1]
Length = 213
Score = 34.0 bits (76), Expect = 1.3
Identities = 24/93 (25%), Positives = 47/93 (49%), Gaps = 4/93 (4%)
Query: 291 NMLIKAQKEVRDSFEDNFNTFKTMSAILNLISVMNQKIDTLTNSTILAVKN----FIEKI 124
N++ K KE+ D ED+ K +S I + + KI+TLT + + +K +K
Sbjct: 61 NIVQKDIKEILDIPEDDPKLKKDLSDIQKTKTSTDAKINTLTKTAVDTIKKESKVVKDKA 120
Query: 123 LRILGVITEEVNKTEDNDVLDILGEYRNAIRKLAAKK 13
++ ++ + V K E N +Y + I+++ +K
Sbjct: 121 PKLAAIVVDNVKKNELNAQKSASSKYVDLIKQIEQEK 157
>gi|1711635|sp|Q10765|SYI_MYCTU PROBABLE ISOLEUCYL-TRNA SYNTHETASE (ISOLEUCINE--TRNA LIGASE)
(ILERS) >gi|1403506|emb|CAA98326| (Z74020) ileS
[Mycobacterium tuberculosis]
Length = 1041
Score = 34.0 bits (76), Expect = 1.3
Identities = 19/55 (34%), Positives = 28/55 (50%)
Query: 609 AYFDKEPWVRYFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLNTP 445
A FD+ + H L DG KMSKSL+N+ + E + S +R F + +P
Sbjct: 596 ALFDRPAFKTCVAHGIVLGFDGQKMSKSLRNYPDVTEVFDRDGSDAMRWFLMASP 650
>gi|3024678|sp|P56457|SYL_HELPY LEUCYL-TRNA SYNTHETASE (LEUCINE--TRNA LIGASE) (LEURS)
>gi|2314727|gb|AAD08585.1| (AE000652) leucyl-tRNA
synthetase (leuS) [Helicobacter pylori 26695]
Length = 806
Score = 33.6 bits (75), Expect = 1.6
Identities = 41/128 (32%), Positives = 65/128 (50%), Gaps = 25/128 (19%)
Query: 564 GHLHID---------------GLKMSKSLKNFITIEESLKKYTSRQLRIFFLNTPYNAPM 430
G+LH+D G KMSKS N ++ +E LKKY + R+F L + AP
Sbjct: 549 GYLHLDEPFKQLITQGMVLKNGAKMSKSKGNVVSPKEILKKYGADAARLFIL---FAAPP 605
Query: 429 TYKIESMEYAKVQEARIFNFLSVAESKIK--DNKNIFKIMTMED-----RMVVNMLIKAQ 271
++E + A R L + I +K FK +++ + R V +K
Sbjct: 606 AKELEWNDSALEGAHRFIKRLYDKANAINPTTSKPEFKEVSLNEAQKLGRKKVYEALKKS 665
Query: 270 KEVRDSFED--NFNT-FKTMSAILNLISVMNQK 181
E+ + E +FNT + LN +S N +
Sbjct: 666 HEIFNKAESAYSFNTLIASCMEALNALSAQNNE 698
>gi|3122909|sp|O28059|SYV_ARCFU VALYL-TRNA SYNTHETASE (VALINE--TRNA LIGASE) (VALRS) >gi|2648303
(AE000952) valyl-tRNA synthetase (valS) [Archaeoglobus
fulgidus]
Length = 863
Score = 33.6 bits (75), Expect = 1.6
Identities = 28/116 (24%), Positives = 52/116 (44%), Gaps = 3/116 (2%)
Query: 591 PWVRYFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLNTPYNAPMTYKIES 412
PW ++ DG KMSKSL N I EE ++KY LR + + + + +
Sbjct: 500 PWYEIVINGMVFGEDGRKMSKSLGNVIVPEEVVEKYGVDALRQWAASGVIGDDIIFNWKD 559
Query: 411 MEYAKVQEARIFNFLSVAESKIKD---NKNIFKIMTMEDRMVVNMLIKAQKEVRDSFED 244
+ A + + ++ I D ++ K++ DR +++ L + EVR ++
Sbjct: 560 VIAASRFQQKFWSITRFTLMHISDYTPSEEDKKLLRDADRWILSKLNRLVGEVRKHMDE 618
>gi|3257481|dbj|BAA30164| (AP000004) 1066aa long hypothetical isoleucyl-tRNA synthetase
[Pyrococcus horikoshii]
Length = 1066
Score = 33.6 bits (75), Expect = 1.6
Identities = 21/50 (42%), Positives = 25/50 (50%)
Query: 603 FDKEPWVRYFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFL 454
FD P+ R MH L G KMSKSL N I EE ++K R + L
Sbjct: 604 FDTVPYRRVAMHGYVLDEKGDKMSKSLGNIIRPEEVVEKAGRDTFRFYML 653
>gi|2496245|sp|Q57611|Y147_METJA HYPOTHETICAL ATP-BINDING PROTEIN MJ0147 >gi|2128154|pir||D64318
hypothetical protein MJ0147 - Methanococcus jannaschii
>gi|1590908 (U67472) M. jannaschii predicted coding
region MJ0147 [Methanococcus jannaschii]
Length = 371
Score = 32.8 bits (73), Expect = 2.8
Identities = 38/150 (25%), Positives = 68/150 (45%), Gaps = 1/150 (0%)
Query: 657 VDLKFPHHENEIHQCQAYFDKEPWVRYFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTS 478
+ +KF + E EIH+ + + EP + YF++ G L+ + K + IE L S
Sbjct: 3 IQMKFFNREKEIHEILSILEGEPNIIYFIY-GPLNSGKTALIKHI-----IENKL----S 52
Query: 477 RQLRIFFLNTPYNAPMTYKIESMEYAKVQEARIFNFLSVAESKIKDNKNIFKIMTMEDRM 298
++F++N TY I E + EA KIKD + ++T ++
Sbjct: 53 DDYKVFYINF-----RTYLIS--EKREFIEAIFTTKKDDFFEKIKDKSEVLNLITKGAKI 105
Query: 297 VVNMLIKAQKEVRDSFEDNFN-TFKTMSAIL 208
+ + I + E FE+ N F+ +++IL
Sbjct: 106 LTGIPI-PEVEFDKLFEEKINDAFQYLNSIL 135
>gi|2984142 (AE000760) preprotein translocase SecA subunit [Aquifex aeolicus]
Length = 984
Score = 32.8 bits (73), Expect = 2.8
Identities = 33/114 (28%), Positives = 56/114 (48%), Gaps = 3/114 (2%)
Query: 354 SKIKDNKNIFKIMTMEDRMVVNMLIKAQKEVRDSFEDNFNTFKTMSAILNLISVMNQKID 175
SK+ D I + +E RMV L AQK V NF K + +++++ +
Sbjct: 699 SKLMDMLKIERGEPIESRMVSKALENAQKRVE---AQNFQIRKRLYEFDSVMNIQRDVVY 755
Query: 174 TLTNSTILAVKNFIEKILRIL-GVITEEVNK--TEDNDVLDILGEYRNAIRKLAAKK 13
TL +L +N EKI L +IT++VN+ ED+ L L + +++L ++
Sbjct: 756 TLRRQ-LLEGENVHEKIKEFLKDIITQKVNELLPEDDPELWDLEPLKAFLKELTGRE 811
>gi|2688027 (AE001125) acriflavine resistance protein (acrB) [Borrelia
burgdorferi]
Length = 1036
Score = 32.5 bits (72), Expect = 3.7
Identities = 24/76 (31%), Positives = 40/76 (52%), Gaps = 4/76 (5%)
Query: 414 SMEYAKVQEARIFNFLSVAESKIKDNKNIFKIMTMEDRMVVNMLIKAQKEVRDSFEDNFN 235
++EYAK R FL + +S+ K K+I + DR+ N+L ++E RD+ + +
Sbjct: 589 NLEYAKFYSNR---FLEIVKSEAKGYKSIIATLRA-DRITFNVLFPLKEESRDNLTQSVD 644
Query: 234 ----TFKTMSAILNLISVMN 187
+K M+ I NL N
Sbjct: 645 YDSIKYKIMNRIGNLYPEFN 664
>gi|2688147 (AE001135) leucyl-tRNA synthetase (leuS) [Borrelia burgdorferi]
Length = 840
Score = 32.5 bits (72), Expect = 3.7
Identities = 33/115 (28%), Positives = 59/115 (50%), Gaps = 1/115 (0%)
Query: 540 KMSKSLKNFITIEESLKKYTSRQLRIFFLNTPYNAPMTYKIESMEYAKVQEARIFNFLSV 361
KMSKSLKN I ++ +K++ + +RI+ + + P+T +S + +F FL
Sbjct: 617 KMSKSLKNVINPDDIIKEFGADSMRIYEM---FMGPLT---DSKPWNTKGIIGVFRFL-- 668
Query: 360 AESKIKDNKNIFKIMTMEDRMVVNMLIKAQKEVRDSFED-NFNTFKTMSAILNLIS 196
+KI + + R +++ L K K+V + E NFNT +SA++ I+
Sbjct: 669 --NKIWNLREKELSKENPPREIISELHKVIKKVTEDTEKLNFNT--AISAMMIFIN 720
>gi|4538961|emb|CAB39785| (AL049488) isoleucine-tRNA ligase-like protein [Arabidopsis
thaliana]
Length = 1254
Score = 32.5 bits (72), Expect = 3.7
Identities = 19/67 (28%), Positives = 36/67 (53%), Gaps = 2/67 (2%)
Query: 609 AYFDKEPWVRYFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLNTPY--NA 436
A F+K + + L DG KM+K L+N+ E + +Y + +R++ +N+P
Sbjct: 609 ALFEKPAFKNLICNGLVLAEDGKKMAKKLRNYPPPLEVIDEYGADAVRLYLINSPVVRAE 668
Query: 435 PMTYKIESM 409
P+ +K E +
Sbjct: 669 PLRFKKEGV 677
>gi|2388946|emb|CAB11680| (Z98978) SPAC27E2.06c, putative methionyl-trna synthetase, le
n:539aa, similar eg. to YGR171C, SYMM_YEAST, P22438,
methi onyl-trna synthetase; mitochondrial, (575aa),
fasta scores, opt:396, E():0, (39.9% identity in 546 aa
o...
Length = 539
Score = 32.1 bits (71), Expect = 4.8
Identities = 46/181 (25%), Positives = 86/181 (47%), Gaps = 26/181 (14%)
Query: 582 RYFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLNT-PYNAPMTYKIESME 406
+ +HS H ++ +KMSKSL N + ++KY +R + L + + IE +E
Sbjct: 313 KILVHS-HWTMNKVKMSKSLGNVVDPFWLIEKYGVDTIRYYLLKRGRLTSDSNFDIEELE 371
Query: 405 YAKVQEARIFNFLSVAESKIKDNK-----NIFKIMTMEDRM-----VVNMLIKAQKEVRD 256
K +E + L V S+++ K I K +D +V+ LI+
Sbjct: 372 --KDEEHDLRRSLGVLLSRLQSKKLFISNEIQKQWHKKDDFTEYEDIVHELIELPVVCAQ 429
Query: 255 SFEDN--FNTFKTMSAILNLISVM-------------NQKIDTLTNSTILAVKNFIEKIL 121
S + + + ++L ++ + +KIDTL + L
Sbjct: 430 SIDGGCVYEVINLVQSVLRRVTKLFQLKEPWKLSDDSQEKIDTLM---------LVAHSL 480
Query: 120 RILGVITEEVNKTEDNDVLDILGEYRN 40
RI G++ + + T+ ++LD LG +N
Sbjct: 481 RISGILLQPIMPTKSTELLDQLGIPKN 507
>gi|4539131|emb|CAB39575| (AL049478) putative isoleucyl-tRNA synthetase [Mycobacterium
leprae]
Length = 1059
Score = 32.1 bits (71), Expect = 4.8
Identities = 18/55 (32%), Positives = 28/55 (50%)
Query: 609 AYFDKEPWVRYFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLNTP 445
A FD+ + H L +G KMSKSL+N+ + E + S +R F + +P
Sbjct: 614 ALFDRPAFKTCIAHGVVLGSNGQKMSKSLRNYPDVTEIFDRDGSDAMRWFLMASP 668
>gi|1351159|sp|P47587|SYI_MYCGE ISOLEUCYL-TRNA SYNTHETASE (ISOLEUCINE--TRNA LIGASE) (ILERS)
>gi|1361704|pir||B64238 isoleucine--tRNA ligase (EC
6.1.1.5) - Mycoplasma genitalium (SGC3) >gi|3844928
(U39716) isoleucyl-tRNA synthetase (ileS) [Mycoplasma
genitalium]
Length = 895
Score = 32.1 bits (71), Expect = 4.8
Identities = 28/99 (28%), Positives = 42/99 (42%)
Query: 600 DKEPWVRYFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLNTPYNAPMTYK 421
D P+ H L +G KMSKSL N + + +Y + LR++ NT +
Sbjct: 570 DLIPFKSLVSHGFTLDENGNKMSKSLGNIVDPLKICDQYGADILRLWVANTDWQIDNKIG 629
Query: 420 IESMEYAKVQEARIFNFLSVAESKIKDNKNIFKIMTMED 304
+ ++ Q RI N L I N N F +M+D
Sbjct: 630 VNILKQVAEQYRRIRNSLL---RFILGNINGFNFTSMDD 665
>gi|2501026|sp|P75398|SYL_MYCPN LEUCYL-TRNA SYNTHETASE (LEUCINE--TRNA LIGASE) (LEURS)
>gi|2146327|pir||S73779 leucine--tRNA ligase (EC 6.1.1.4)
leuS - Mycoplasma pneumoniae (SGC3) (ATCC 29342)
>gi|1674141 (AE000044) Mycoplasma pneumoniae, leucyl-tRNA
synthetase; similar to Swiss-Prot Accession Number
P36430, from B. subtilis [Mycoplasma pneumoniae]
Length = 793
Score = 32.1 bits (71), Expect = 4.8
Identities = 52/213 (24%), Positives = 85/213 (39%), Gaps = 10/213 (4%)
Query: 726 WHIECSAMAADIFGDEL--DIHTGAVDLKFPH------HENEIHQCQAYFDKEPWVRYFM 571
W I+ S A D+F L D++ G + H ++Q KEP+ +
Sbjct: 500 WPID-SREAKDLFERYLPVDLYVGGAEHAVLHLLYARFWHQFLYQKGIVTTKEPFKKLIN 558
Query: 570 HSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFL-NTPYNAPMTYKIESMEYAKV 394
L DG KMSKS N I + + + LR++ + P +A +T+ + + +
Sbjct: 559 QGMVLGPDGKKMSKSKGNTINPTPLIDSHGADALRLYLMFMGPISAALTWLDDGLNGMRR 618
Query: 393 QEARIFNFLSVAESKIKDNKNIFK-IMTMEDRMVVNMLIKAQKEVRDSFEDNFNTFKTMS 217
R+ NF +NI K + E N+ +K E + E N M
Sbjct: 619 WLDRVHNFF--------HKENIIKETVDQETVYGYNLFLKRSFEHLEKQELNL-VISQMM 669
Query: 216 AILNLISVMNQKIDTLTNSTILAVKNFIEKILRILGVITEEVN 88
LNL+ Q T+ K F+ + + EE+N
Sbjct: 670 IFLNLLYKTKQ-------LTLAYAKGFLTVLSFFAPYLAEELN 705
>gi|3758845|emb|CAB11130| (Z98551) predicted using hexExon; MAL3P6.21 (PFC0810c),
Hypothetical protein, len: 1120 aa [Plasmodium
falciparum]
Length = 1119
Score = 32.1 bits (71), Expect = 4.8
Identities = 38/164 (23%), Positives = 74/164 (44%), Gaps = 9/164 (5%)
Query: 606 YFDKEPWVRYFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFLNTPYNAPMT 427
Y D + ++ F H + ++DG K I++ L + + I+ N ++
Sbjct: 364 YLDIQQDIKSFFHESNRNVDGSK----------IQDMLFHFERKNNSIYQNNMFHDKYYK 413
Query: 426 YKIESMEYAKVQEARIFNFLSVAESKIKDNKNIFKIMTMEDRMVVNMLIKAQKEVRDSFE 247
KI+ ++E + N +S K NKNI ++ + + N+ +QKE F
Sbjct: 414 KKIKK----DIKEEKKQN-----DSLQKYNKNIDPLLFIRKK---NIFFDSQKEYYKKFL 461
Query: 246 DNFNTFKTMSAILNLISVMNQKIDTLTN---------STILAVKNFIEKILRI 115
++ + ++ + N S N +I L N + LA+KN ++K +I
Sbjct: 462 SSYTYEEIVNILCNKSSRCNNRIFQLINDVNKNTFILKSHLALKNELKKFFQI 514
>gi|2983452 (AE000714) arginyl-tRNA synthetase [Aquifex aeolicus]
Length = 583
Score = 32.1 bits (71), Expect = 4.8
Identities = 22/94 (23%), Positives = 43/94 (45%), Gaps = 9/94 (9%)
Query: 543 LKMSKSLKNFITIEESLKKYTSRQLRIFFLNTPYNAPMTYKIESME---------YAKVQ 391
+KMSK F+T+ E L + +R FL + P+ + +E + Y +
Sbjct: 405 VKMSKRAGTFVTLRELLDEVGKDAVRFIFLTKRSDTPLDFDVEKAKEKSSENPVYYVQYA 464
Query: 390 EARIFNFLSVAESKIKDNKNIFKIMTMEDRMVVNMLIKAQKEV 262
ARI + + K + ++ +++ + IK K+V
Sbjct: 465 HARISGIFREFKERYKKDVSVEELINYVQHLEEEAEIKLIKKV 507
>gi|4155204 (AE001497) RNA POLYMERASE SIGMA-54 FACTOR [Helicobacter pylori J99]
Length = 414
Score = 32.1 bits (71), Expect = 4.8
Identities = 33/120 (27%), Positives = 56/120 (46%), Gaps = 7/120 (5%)
Query: 387 ARIFNFLSVAESKIKDNKNIFKIMTMEDRMVVNMLIKAQKEVRDSFEDNFNTFKTMSAIL 208
A + +L + +S+++D + + K ++ N LIK + + +F D FNT K+ +
Sbjct: 16 ATLKGWLPILQSELEDLEEVLKQNALD-----NPLIKIENKRIKNFSDRFNTKKSSDHLE 70
Query: 207 NLISVMNQKIDTLTNSTI--LAVKNFIEKILR--ILGVITE---EVNKTEDNDVLDILGE 49
N + +TL + I L +KI I G+ E E N E VL + E
Sbjct: 71 NCATASKSLFETLESQIIPPLFPTETSQKIAMDIISGLNNEGYFEENIEERAKVLGVESE 130
Query: 48 YRNAIRK 28
+RK
Sbjct: 131 VYEKVRK 137
>gi|2496959|sp|Q09307|YQR2_CAEEL HYPOTHETICAL 45.1 KD PROTEIN F19C6.2 IN CHROMOSOME X
>gi|3876082|emb|CAA88050| (Z48006) cDNA EST EMBL:D70601
comes from this gene; cDNA EST EMBL:D66703 comes from
this gene; cDNA EST yk474e8.3 comes from this gene
[Caenorhabditis elegans]
Length = 495
Score = 31.7 bits (70), Expect = 6.3
Identities = 44/211 (20%), Positives = 80/211 (37%), Gaps = 6/211 (2%)
Query: 756 ESKWGEGRPGWHIECSAMAADIFGDELDIH--TGAVDLKFPHHENEIHQCQAYFDKEPWV 583
E+K + P W A ++FG IH + V KFP
Sbjct: 97 EAKKSDVLPNWAHSVKDFAENVFGMNGVIHLISPTVSEKFPKRSQ--------------- 141
Query: 582 RYFMHSGHLHIDGLK-MSKSLKNFITIEESLKKYTSRQLRIFFL-NTPYNAPMTYKIESM 409
ID L+ + +N + E +K S + R F + NT N P++ +
Sbjct: 142 ----------IDILRAVPNETRNILVTESLSEKLHSSESRNFGMHNTSVNVPVSMAYVAR 191
Query: 408 EYAKVQEARIFNFLSVAESKIKDNKNIFKIMTMEDRMVVNMLIKAQ--KEVRDSFEDNFN 235
E + I F+ E KIK+ + ++ DR++++ +I KEV +
Sbjct: 192 ERPDILSLAIREFVKTDERKIKELEK--RLANEHDRVMIHTIINDTDWKEVTAVADIESP 249
Query: 234 TFKTMSAILNLISVMNQKIDTLTNSTILAVKNFIEKI 124
T + + ++K ++ N + V +K+
Sbjct: 250 TDIVSHRVSLALLAFDEKHSSIPNGVDVPVSGLFQKV 286
>gi|2507608|sp|P47508|SYL_MYCGE LEUCYL-TRNA SYNTHETASE (LEUCINE--TRNA LIGASE) (LEURS)
>gi|1361707|pir||D64229 leucine--tRNA ligase (EC
6.1.1.4) - Mycoplasma genitalium (SGC3) >gi|3844862
(U39706) leucyl-tRNA synthetase (leuS) [Mycoplasma
genitalium]
Length = 792
Score = 31.7 bits (70), Expect = 6.3
Identities = 22/76 (28%), Positives = 37/76 (47%), Gaps = 1/76 (1%)
Query: 597 KEPWVRYFMHSGHLHIDGLKMSKSLKNFITIEESLKKYTSRQLRIFFL-NTPYNAPMTYK 421
KEP+ + L DG KMSKS N I + + + LR++ + P +A +T+
Sbjct: 550 KEPFQKLINQGMVLGPDGKKMSKSKGNTINPTPLVDSHGADALRLYLMFMGPISASLTWN 609
Query: 420 IESMEYAKVQEARIFNF 370
E + + R++NF
Sbjct: 610 DEGLNGMRRWLDRVYNF 626
>gi|457146 (L27838) rhoptry protein [Plasmodium yoelii]
Length = 2269
Score = 31.3 bits (69), Expect = 8.3
Identities = 34/115 (29%), Positives = 53/115 (45%), Gaps = 18/115 (15%)
Query: 375 NFLSVAESKIKDNKNIFKIMTMEDRMVVNMLIKAQKEVRDSFEDNFNTFKTM-------- 220
N L + ES++ N N K + + N + A E NFNT K +
Sbjct: 1810 NILQLQESELTANLNDLKEIGQKISDKKNKFLHALNETPIP---NFNTLKEIYHDIVKYK 1866
Query: 219 ---SAILNLISVMNQKIDTLTNSTILAVKNFIEKILRIL-------GVITEEVNKTEDND 70
I N+ S N+ I TL TI +K ++ IL + +I + + T +ND
Sbjct: 1867 RQIDEIENITSEENENI-TLYIDTITKLKEKVQSILNFVTTYENDSNIIKQHIQDTNEND 1925
Query: 69 VLDILGEYRNAIR 31
V I + I+
Sbjct: 1926 VSKIKESLKTTIQ 1938
Score = 31.3 bits (69), Expect = 8.3
Identities = 40/160 (25%), Positives = 79/160 (49%), Gaps = 30/160 (18%)
Query: 513 ITIEESLKKYTSRQLRIF-----FLNTPYNAPMTYKIESMEYAKVQEARIFNFLSVAESK 349
I +E+L KY + I+ N N T ES+ Y ++Q RI +
Sbjct: 1251 IDSDENLGKYNTEMGNIYEEFIKSYNLITNYLETVSKESITYNQIQNKRI----DTQKEL 1306
Query: 348 IKDNKNIFKIMTMEDRM-------VVNMLIKAQKEVRDSFEDNF--------------NT 232
+K+ +N+ K + D + +V K V D+F++ + NT
Sbjct: 1307 LKNIENVNKAKSYLDYIKENEFDRIVTHFKKKLNTVNDNFKNEYSKVNEGFDNISNSINT 1366
Query: 231 FKTMS---AILNLISVMNQKIDTLTNSTILAVKNFIEKILRILGVITEEVN-KTEDNDVL 64
K + ++LN+++ + + N+T + K E I R + + +N K +++ +
Sbjct: 1367 VKNSTDENSLLNILNQTKEMYANIVNNTYYSYKYEAENIFRNIPKLANTLNIKIKNSSGI 1426
Query: 63 DILGEYRNAI 34
D+ + + AI
Sbjct: 1427 DLSKDIKIAI 1436
>gi|3881411|emb|CAB00882| (Z77136) cDNA EST EMBL:C12394 comes from this gene; cDNA EST
EMBL:C10616 comes from this gene; cDNA EST yk348g4.3
comes from this gene; cDNA EST yk348g4.5 comes from this
gene; cDNA EST yk194h9.5 comes from this gene; cDNA EST
...
Length = 1152
Score = 31.3 bits (69), Expect = 8.3
Identities = 30/122 (24%), Positives = 61/122 (49%), Gaps = 8/122 (6%)
Query: 504 EESLKKYTSRQLRIFFLNTPYNAPMTYKIESMEYAK---VQEARIFNFLSVAESKIKDNK 334
E K T+R +F + P M E+ E A ++E + + ++ +K+KD K
Sbjct: 721 ENVRSKITARAAVLFSYSLPTPESMQIVAENHENASAEVIEEEQFGSNINQRINKLKDIK 780
Query: 333 NIFKIMTMEDRMVVNMLIKAQKEVRDSFEDNFNTFKTMSAILNLISVMN--QKIDTLT-- 166
NI I+ + +R V M + + + D F ++ N+ L+++ + +++D +
Sbjct: 781 NIKIILEVINRSSVRMQLASLPIIIDIFMESLNSSTPQGFEQPLMAIFDRYEQLDPFSLF 840
Query: 165 -NSTILAVKN 139
STI ++N
Sbjct: 841 QQSTIAWIRN 850
>gi|586388|sp|P38348|YB9Q_YEAST HYPOTHETICAL 55.5 KD PROTEIN IN MRPL37-RIF1 INTERGENIC REGION
>gi|626246|pir||S46153 probable membrane protein YBR272c
- yeast (Saccharomyces cerevisiae)
>gi|536709|emb|CAA85235| (Z36141) ORF YBR272c
[Saccharomyces cerevisiae]
Length = 480
Score = 31.3 bits (69), Expect = 8.3
Identities = 34/158 (21%), Positives = 77/158 (48%), Gaps = 13/158 (8%)
Query: 522 KNFITIEESLKKYTSRQL---RIFFLNTPYNAPMTYKIESMEYAKVQEARIFNFLSVAES 352
K IE++L++ ++ +L R+F N PY + ++E++ + ++ + F ++
Sbjct: 151 KLITAIEKALERLSTDELIRRRLFDNNLPYLVSVKGRMETVSFVRLIDFLTIEFQFISGP 210
Query: 351 KIKDNKNIFK----IMTMEDRMV----VNMLIKAQKEVRDSFEDNFNTFKTMSAILNLIS 196
+ KD F + ++ED +V VN K E+R+ +D + + + IL + +
Sbjct: 211 EFKDIIFCFTKEEILKSVEDILVFIELVNYYTKFLLEIRN--QDKYWALRHVKKILPVFA 268
Query: 195 VMNQKIDTLTNSTILAVKNFIEKILRILGVITEEVN--KTEDNDVLDILGE 49
+ + + + + ++ + + +E + KT D D L I E
Sbjct: 269 QLFEDTENYPDVRAFSTNCLLQLFAEVSRIEEDEYSLFKTMDKDSLKIGSE 319
>gi|780502 (U18466) AP endonuclease class II [African swine fever virus]
>gi|1097525|prf||2113434ET AP endonuclease:ISOTYPE=class
II [African swine fever virus]
Length = 296
Score = 31.3 bits (69), Expect = 8.3
Identities = 18/45 (40%), Positives = 23/45 (51%)
Query: 507 IEESLKKYTSRQLRIFFLNTPYNAPMTYKIESMEYAKVQEARIFN 373
I E LKK + + +L TP+N TYK +ME K RI N
Sbjct: 124 IVEGLKKIKPVEGVVIYLETPHNKHHTYKYSTMEQIKELFLRIRN 168
Database: Non-redundant GenBank CDS
translations+PDB+SwissProt+SPupdate+PIR
Posted date: Apr 17, 1999 5:33 AM
Number of letters in database: 113,023,754
Number of sequences in database: 369,800
Lambda K H
0.318 0.135 0.00
Gapped
Lambda K H
0.270 0.0470 4.94e-324
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 138310476
Number of Sequences: 369800
Number of extensions: 2768517
Number of successful extensions: 11507
Number of sequences better than 10.0: 176
Number of HSP's better than 10.0 without gapping: 61
Number of HSP's successfully gapped in prelim test: 39
Number of HSP's that attempted gapping in prelim test: 11397
Number of HSP's gapped (non-prelim): 123
length of query: 266
length of database: 113023754
effective HSP length: 54
effective length of query: 211
effective length of database: 93054554
effective search space: 19634510894
effective search space used: 19634510894
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 68 (30.9 bits)