BLASTX 2.0.8 [Jan-05-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 1449F
(882 letters)
Database: Non-redundant GenBank CDS
translations+PDB+SwissProt+SPupdate+PIR
368,476 sequences; 112,640,273 total letters
Searching...................................................done
Score E
Sequences producing significant alignments: (bits) Value
gi|2920561 (AF019228) chitin synthase [Spraguea lophii] 268 5e-92
gi|2443816 (AF020528) Class IV chitin synthase [Magnaporthe grisea] 52 6e-06
gi|2133265|pir||JC6079 chitin synthase (EC 2.4.1.16) - Emericell... 50 2e-05
gi|1272475 (U52362) chitin synthase [Emericella nidulans] 50 2e-05
gi|3360|emb|CAA40559| (X57300) chitin synthase [Saccharomyces ce... 48 1e-04
gi|172104 (M73697) chitin synthase [Saccharomyces cerevisiae] 48 1e-04
gi|584947|sp|P29465|CHS3_YEAST CHITIN SYNTHASE 3 (CHITIN-UDP ACE... 48 1e-04
gi|2444457 (AF021318) chitin synthase [Filobasidiella neoformans] 43 0.003
gi|2493681|sp|Q01285|CHS4_NEUCR CHITIN SYNTHASE 4 (CHITIN-UDP AC... 42 0.006
gi|3978549|dbj|BAA34808| (AB004547) Chs3 [Rhizopus microsporus v... 41 0.008
gi|4218177|emb|CAA08762| (AJ009630) chitin synthase [Glomus vers... 41 0.008
gi|231792|sp|P30573|CHS3_CANAL CHITIN SYNTHASE 3 (CHITIN-UDP ACE... 40 0.025
gi|742325|prf||2009366A chitin synthase 3 [Candida albicans] 40 0.025
gi|2613106 (AF030553) class IV chitin synthase [Ustilago maydis] 36 0.48
gi|2258376 (AF007261) Orf717 [Reclinomonas americana] 35 0.83
gi|3097043|emb|CAA75298| (Y15035) B22R [Cowpox virus] 32 7.2
>gi|2920561 (AF019228) chitin synthase [Spraguea lophii]
Length = 185
Score = 268 bits (677), Expect = 5e-92
Identities = 130/142 (91%), Positives = 130/142 (91%)
Query: 327 MQRPSNSELLRSSSRTLFRQDILRDDHAKSFWYNIXPIFTFLIPDCILRCLGIKSKSSIT 506
MQRPSNSELLRSSSRTLFRQDILRDDHAKSFWYNI PIFTFLIPDCILRCLGIKSKSSIT
Sbjct: 1 MQRPSNSELLRSSSRTLFRQDILRDDHAKSFWYNIXPIFTFLIPDCILRCLGIKSKSSIT 60
Query: 507 AWREKVAXXXXXXXXXXXXGFFTYGINIFLCHGNNNFIYGKLKLKKNYVIANGGIYYTDE 686
AWREKVA GFFTYGINIFLCHGNNNFIYGKLKLKKNYVIANGGIYYTDE
Sbjct: 61 AWREKVALCSIISMLCLILGFFTYGINIFLCHGNNNFIYGKLKLKKNYVIANGGIYYTDE 120
Query: 687 YENSSIIYLGSKNTGNLVIWIF 752
YENSSIIYLGSKNTGNLVIWIF
Sbjct: 121 YENSSIIYLGSKNTGNLVIWIF 142
Score = 92.1 bits (225), Expect = 5e-92
Identities = 43/43 (100%), Positives = 43/43 (100%)
Query: 752 LSKQDMVRVGDIYFYTSDVLNRYFIIRENKIFNPAVFLEYGGD 880
LSKQDMVRVGDIYFYTSDVLNRYFIIRENKIFNPAVFLEYGGD
Sbjct: 143 LSKQDMVRVGDIYFYTSDVLNRYFIIRENKIFNPAVFLEYGGD 185
>gi|2443816 (AF020528) Class IV chitin synthase [Magnaporthe grisea]
Length = 1198
Score = 51.9 bits (122), Expect = 6e-06
Identities = 31/114 (27%), Positives = 53/114 (46%)
Query: 342 NSELLRSSSRTLFRQDILRDDHAKSFWYNIXPIFTFLIPDCILRCLGIKSKSSITAWREK 521
NS++++ R ++ I S W + TF PD I++C G+ +K+ AWREK
Sbjct: 184 NSKIVKEGKR---KEKIPEQLRPPSAWNVYCAVITFWSPDFIMKCCGMPAKAQRRAWREK 240
Query: 522 VAXXXXXXXXXXXXGFFTYGINIFLCHGNNNFIYGKLKLKKNYVIANGGIYYTD 683
+ GF T+G N +C G ++ + ++Y+I +G Y D
Sbjct: 241 IGLISLILIIMGVVGFLTFGFNQAVCGGPVLRLHIN-SVDRSYMIFHGTAYNLD 293
>gi|2133265|pir||JC6079 chitin synthase (EC 2.4.1.16) - Emericella nidulans
>gi|1688026|dbj|BAA11866| (D83246) chitin synthase
[Emericella nidulans]
Length = 1086
Score = 50.4 bits (118), Expect = 2e-05
Identities = 26/98 (26%), Positives = 42/98 (42%)
Query: 381 RQDILRDDHAKSFWYNIXPIFTFLIPDCILRCLGIKSKSSITAWREKVAXXXXXXXXXXX 560
R+ + S W + TF PD +L+C G+ K+ +AWREK+
Sbjct: 76 RRKAMEQVRPPSLWTTYCSVITFWAPDFVLKCFGMPQKAQRSAWREKIGLISIILMIAAF 135
Query: 561 XGFFTYGINIFLCHGNNNFIYGKLKLKKNYVIANGGIY 674
GF T+G +C G + ++ Y+I +G Y
Sbjct: 136 VGFLTFGFTATVC-GTPSTRLKINEIGSGYMIFHGQAY 172
>gi|1272475 (U52362) chitin synthase [Emericella nidulans]
Length = 1184
Score = 50.0 bits (117), Expect = 2e-05
Identities = 21/73 (28%), Positives = 32/73 (43%)
Query: 381 RQDILRDDHAKSFWYNIXPIFTFLIPDCILRCLGIKSKSSITAWREKVAXXXXXXXXXXX 560
R+ + S W + TF PD +L+C G+ K+ +AWREK+
Sbjct: 174 RRKAMEQVRPPSLWTTYCSVITFWAPDFVLKCFGMPQKAQRSAWREKIGLISIILMIAAF 233
Query: 561 XGFFTYGINIFLC 599
GF T+G +C
Sbjct: 234 VGFLTFGFTATVC 246
>gi|3360|emb|CAA40559| (X57300) chitin synthase [Saccharomyces cerevisiae]
Length = 1099
Score = 47.6 bits (111), Expect = 1e-04
Identities = 33/97 (34%), Positives = 40/97 (41%), Gaps = 5/97 (5%)
Query: 414 SFWYNIXPIFTFLIPDCILRCLGIKSKSSITAWREKVAXXXXXXXXXXXXGFFTYGINIF 593
SFW TF P IL G+ K AWREKVA F T+G
Sbjct: 101 SFWQMYCYFITFWAPAPILAFCGMPKKERQMAWREKVALISVILYIGAIVAFLTFGFTKT 160
Query: 594 LCHGNNNFIYGKLKLKKN-----YVIANGGIYYTDEYENSSI 704
+C + KL+LK N +V+ NG Y D S I
Sbjct: 161 VCSSS------KLRLKNNEVSTEFVVINGKAYELDTSSRSGI 196
>gi|172104 (M73697) chitin synthase [Saccharomyces cerevisiae]
Length = 1165
Score = 47.6 bits (111), Expect = 1e-04
Identities = 33/97 (34%), Positives = 40/97 (41%), Gaps = 5/97 (5%)
Query: 414 SFWYNIXPIFTFLIPDCILRCLGIKSKSSITAWREKVAXXXXXXXXXXXXGFFTYGINIF 593
SFW TF P IL G+ K AWREKVA F T+G
Sbjct: 167 SFWQMYCYFITFWAPAPILAFCGMPKKERQMAWREKVALISVILYIGAIVAFLTFGFTKT 226
Query: 594 LCHGNNNFIYGKLKLKKN-----YVIANGGIYYTDEYENSSI 704
+C + KL+LK N +V+ NG Y D S I
Sbjct: 227 VCSSS------KLRLKNNEVSTEFVVINGKAYELDTSSRSGI 262
>gi|584947|sp|P29465|CHS3_YEAST CHITIN SYNTHASE 3 (CHITIN-UDP ACETYL-GLUCOSAMINYL TRANSFERASE 3)
>gi|626127|pir||S45879 chitin synthase (EC 2.4.1.16) 3 -
yeast (Saccharomyces cerevisiae)
>gi|498752|emb|CAA53680| (X76078) chitin synthase 3
[Saccharomyces cerevisiae] >gi|536230|emb|CAA84965|
(Z35892) ORF YBR023c [Saccharomyces cerevisiae]
>gi|1587581|prf||2206497D chitin synthase 3
[Saccharomyces cerevisiae]
Length = 1165
Score = 47.6 bits (111), Expect = 1e-04
Identities = 33/97 (34%), Positives = 40/97 (41%), Gaps = 5/97 (5%)
Query: 414 SFWYNIXPIFTFLIPDCILRCLGIKSKSSITAWREKVAXXXXXXXXXXXXGFFTYGINIF 593
SFW TF P IL G+ K AWREKVA F T+G
Sbjct: 167 SFWQMYCYFITFWAPAPILAFCGMPKKERQMAWREKVALISVILYIGAIVAFLTFGFTKT 226
Query: 594 LCHGNNNFIYGKLKLKKN-----YVIANGGIYYTDEYENSSI 704
+C + KL+LK N +V+ NG Y D S I
Sbjct: 227 VCSSS------KLRLKNNEVSTEFVVINGKAYELDTSSRSGI 262
>gi|2444457 (AF021318) chitin synthase [Filobasidiella neoformans]
Length = 1041
Score = 43.0 bits (99), Expect = 0.003
Identities = 25/63 (39%), Positives = 31/63 (48%), Gaps = 2/63 (3%)
Query: 444 TFLIPDCILRCLGIKSKSSITAWREKVAXXXXXXXXXXXXGFFTYGINIFLCHGN--NNF 617
TF P +LR GIK K S AWREK+A GF T G+ LC + N
Sbjct: 83 TFWAPPVLLRWFGIKEKQSRQAWREKIALCWIAVLLGGVVGFVTMGLQRALCPADQQNQS 142
Query: 618 IYGKL 632
+Y +L
Sbjct: 143 MYQRL 147
>gi|2493681|sp|Q01285|CHS4_NEUCR CHITIN SYNTHASE 4 (CHITIN-UDP ACETYL-GLUCOSAMINYL TRANSFERASE 4)
>gi|2133308|pir||S61886 chitin synthase class IV -
Neurospora crassa >gi|793938 (U25097) chitin synthase
[Neurospora crassa]
Length = 1195
Score = 41.8 bits (96), Expect = 0.006
Identities = 27/93 (29%), Positives = 40/93 (42%), Gaps = 2/93 (2%)
Query: 414 SFWYNIXPIFTFLIPDCILRCLGIKSKSSITAWREKVAXXXXXXXXXXXXGFFTYGINIF 593
SFW TF P +L+C G+ + AWREK+ GF T+G
Sbjct: 169 SFWNIYCGFVTFWCPGFVLKCFGMPEMAQQRAWREKMGLISIILLIMGFVGFITFGFTQV 228
Query: 594 LCHGNNNFIYGK--LKLKKNYVIANGGIYYTDEYE 692
+C GK L+L+ N V + I++ Y+
Sbjct: 229 VC--------GKPPLRLRINEVGSGYMIFHGSAYD 255
>gi|3978549|dbj|BAA34808| (AB004547) Chs3 [Rhizopus microsporus var. oligosporus]
Length = 1112
Score = 41.4 bits (95), Expect = 0.008
Identities = 27/108 (25%), Positives = 47/108 (43%)
Query: 414 SFWYNIXPIFTFLIPDCILRCLGIKSKSSITAWREKVAXXXXXXXXXXXXGFFTYGINIF 593
S W + T P +L+ GIK K WREKV+ GF T+G N
Sbjct: 114 SCWVTTTWVLTCWTPPVLLKACGIKDKGMQQIWREKVSLVMIIVFLCAFVGFLTFGFNAT 173
Query: 594 LCHGNNNFIYGKLKLKKNYVIANGGIYYTDEYENSSIIYLGSKNTGNL 737
+C N I +++++++ +G + + + S + G +G+L
Sbjct: 174 VCGITPNRI-RPTGVEESHIVISGRAFDLKTFRHPSPV-PGMPQSGDL 219
>gi|4218177|emb|CAA08762| (AJ009630) chitin synthase [Glomus versiforme]
Length = 1194
Score = 41.4 bits (95), Expect = 0.008
Identities = 31/102 (30%), Positives = 43/102 (41%), Gaps = 6/102 (5%)
Query: 420 WYNIXPIFTFLIPDCILRCLGIKSKSSITAWREKVAXXXXXXXXXXXXGFFTYGINIFLC 599
W I TF +P +L+ G + AWREK+ GF T+G +C
Sbjct: 208 WTAYCSIITFWVPGFLLKVFGKPQPAQQQAWREKIGLISIILFMCAFVGFLTFGFTQAVC 267
Query: 600 HGNNNFIYGKLKLKKNYV-----IANGGIYYTDEYENSSII-YLGSKN 725
L+LK NYV I +G Y T + + + LG KN
Sbjct: 268 G------IPPLRLKVNYVDTGYLIIHGRAYDTSKSNHPPALGILGGKN 309
>gi|231792|sp|P30573|CHS3_CANAL CHITIN SYNTHASE 3 (CHITIN-UDP ACETYL-GLUCOSAMINYL TRANSFERASE 3)
>gi|218362|dbj|BAA02707| (D13454) chitin synthase III
[Candida albicans]
Length = 1213
Score = 39.9 bits (91), Expect = 0.025
Identities = 27/113 (23%), Positives = 49/113 (42%), Gaps = 1/113 (0%)
Query: 336 PSNSELLRSSSRTLFRQDILRDDHAKSFWYNIXPIFTFLIPDCILRCLGIKSKSSITAWR 515
P ++++ SSSR + + + H FW TF P +L+ G+ +K AWR
Sbjct: 140 PKKNQVI-SSSRPMNNEKPAKPKHDIYFWKVYCYAITFWAPAPLLKLFGLPTKDRQFAWR 198
Query: 516 EKVAXXXXXXXXXXXXGFFTYGINIFLCHGNNNFIYGKLK-LKKNYVIANGGIY 674
EK+ + T+G +C ++ + ++ + Y+I NG Y
Sbjct: 199 EKIGLISCILYVGAFVAYLTFGFTKTVC--SSQVVRTQINHVNGGYLIINGRAY 250
>gi|742325|prf||2009366A chitin synthase 3 [Candida albicans]
Length = 1175
Score = 39.9 bits (91), Expect = 0.025
Identities = 27/113 (23%), Positives = 49/113 (42%), Gaps = 1/113 (0%)
Query: 336 PSNSELLRSSSRTLFRQDILRDDHAKSFWYNIXPIFTFLIPDCILRCLGIKSKSSITAWR 515
P ++++ SSSR + + + H FW TF P +L+ G+ +K AWR
Sbjct: 102 PKKNQVI-SSSRPMNNEKPAKPKHDIYFWKVYCYAITFWAPAPLLKLFGLPTKDRQFAWR 160
Query: 516 EKVAXXXXXXXXXXXXGFFTYGINIFLCHGNNNFIYGKLK-LKKNYVIANGGIY 674
EK+ + T+G +C ++ + ++ + Y+I NG Y
Sbjct: 161 EKIGLISCILYVGAFVAYLTFGFTKTVC--SSQVVRTQINHVNGGYLIINGRAY 212
>gi|2613106 (AF030553) class IV chitin synthase [Ustilago maydis]
Length = 1239
Score = 35.6 bits (80), Expect = 0.48
Identities = 18/60 (30%), Positives = 23/60 (38%)
Query: 420 WYNIXPIFTFLIPDCILRCLGIKSKSSITAWREKVAXXXXXXXXXXXXGFFTYGINIFLC 599
W I T P LR GI++ AWREK+ GF T+G +C
Sbjct: 208 WMIYCYILTICCPGPFLRIFGIRTPEQQRAWREKMGLIGIITLIMAAVGFLTFGFTQTVC 267
>gi|2258376 (AF007261) Orf717 [Reclinomonas americana]
Length = 717
Score = 34.8 bits (78), Expect = 0.83
Identities = 19/61 (31%), Positives = 38/61 (62%), Gaps = 2/61 (3%)
Query: 595 KNIFIPYV--KNPKIKHNIEIIEHNATFSLHAVILLLLFIPKHRNIQSGIRNVKIGXILY 422
KNI + Y+ KN K+N+ + + FS+ ++L+L F+ +H N + G +++ I +++
Sbjct: 77 KNIGLYYIYKKNKLKKNNLLYVYSHEFFSIQIIVLILEFVNRHLNYRIGTKDL-IFLLMF 135
Query: 421 QND 413
ND
Sbjct: 136 LND 138
>gi|3097043|emb|CAA75298| (Y15035) B22R [Cowpox virus]
Length = 1933
Score = 31.7 bits (70), Expect = 7.2
Identities = 15/46 (32%), Positives = 26/46 (55%)
Query: 576 YGINIFLCHGNNNFIYGKLKLKKNYVIANGGIYYTDEYENSSIIYL 713
Y I+ +LC +++IY ++ K+YV+ N D E+S I+ L
Sbjct: 1762 YPIDNYLCGTLDDYIYTSVEYNKSYVLVNDTYMSYDYLESSGIVVL 1807
Database: Non-redundant GenBank CDS
translations+PDB+SwissProt+SPupdate+PIR
Posted date: Apr 12, 1999 12:54 PM
Number of letters in database: 112,640,273
Number of sequences in database: 368,476
Lambda K H
0.318 0.135 0.00
Gapped
Lambda K H
0.270 0.0470 4.94e-324
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 129845802
Number of Sequences: 368476
Number of extensions: 2210801
Number of successful extensions: 5004
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 16
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 4985
Number of HSP's gapped (non-prelim): 19
length of query: 294
length of database: 112640273
effective HSP length: 54
effective length of query: 239
effective length of database: 92742569
effective search space: 22165473991
effective search space used: 22165473991
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 69 (31.3 bits)