BLASTX 2.0.8 [Jan-05-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 14-13R
(743 letters)
Database: Non-redundant GenBank CDS
translations+PDB+SwissProt+SPupdate+PIR
369,800 sequences; 113,023,754 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gi|1203939 (U49830) Similar to protein tyrosine phosphatase [Cae... 39 0.045
gi|3413|emb|CAA34421| (X16385) Baf1 protein (AA 1-731) [Saccharo... 36 0.23
gi|114814|sp|P14164|BAF1_YEAST TRANSCRIPTION FACTOR BAF1 (ARS BI... 36 0.23
gi|320637|pir||S29870 DNA-binding protein BAF1 - yeast (Saccharo... 36 0.23
gi|3822238|gb|AAC70189|AAC70189 (AF081810) mucin-like protein [L... 32 4.5
gi|3024085|sp|Q28824|KMLS_BOVIN MYOSIN LIGHT CHAIN KINASE, SMOOT... 32 5.9
gi|3914889|sp|O51453|RS13_BORBU 30S RIBOSOMAL PROTEIN S13 >gi|26... 31 7.7
>gi|1203939 (U49830) Similar to protein tyrosine phosphatase [Caenorhabditis
elegans]
Length = 815
Score = 38.7 bits (88), Expect = 0.045
Identities = 43/129 (33%), Positives = 61/129 (46%), Gaps = 17/129 (13%)
Query: 446 CLTAIVEENTEYDGLVKVYPTTTSPKLLSMMTYFDNS--AKPGFVTTKKKVGFKDISNSG 273
C T + NT D +VY T P+ T DN+ +K G +KKK S
Sbjct: 338 CATLTFDHNTPGDHFERVYVNITLPE----KTGQDNNQGSKKGQTDSKKKD-----QGSR 388
Query: 272 YTYGKNKKEDGWQYVMSRQKS-------------IDEIKSSAKTLNP--WGRLKSSENPF 138
G +KK++ + R+ S I KS +P +GR K+ EN
Sbjct: 389 KARGNSKKKNHSSKKVVRKTSKKKKNAASPNAQLIPVAKSPGNDKSPAGFGRKKAGENLN 448
Query: 137 ETIKPLKTPSPSENSPLESANPFKTT 60
E +K L+ P+P LE N +KTT
Sbjct: 449 EVMKHLRAPTPRAQQELEKTN-WKTT 473
>gi|3413|emb|CAA34421| (X16385) Baf1 protein (AA 1-731) [Saccharomyces cerevisiae]
Length = 731
Score = 36.4 bits (82), Expect = 0.23
Identities = 38/143 (26%), Positives = 64/143 (44%), Gaps = 9/143 (6%)
Query: 572 KEDASLASALKKDIPVSSGDSHKKDLPLSGGISYSGXNIPKKCLTAIVEENTEYDGLVKV 393
+++ SL + IP + K D L + S + K ++ VEE+ D L++
Sbjct: 343 EDNLSLGKFILTKIPKILQNDLKFDQILESSYNNSNHTVSKFKVSHYVEESGLLDILMQR 402
Query: 392 YPTTTSP---KLLSMMTYFDNSAKPGFVTTKKKVG-FKDISNSGYTYGKNKKEDGWQYVM 225
Y T +LLS + + K FV KKK+G + D+ S + N +
Sbjct: 403 YGLTAEDFEKRLLSQIARRITTYKARFVLKKKKMGEYNDLQPSSSSNNNNNNDGELSGTN 462
Query: 224 SRQKSID-----EIKSSAKTLNPWGRLKSSEN 144
R SID EI S+ + N + +++N
Sbjct: 463 LRSNSIDYAKHQEISSAGTSSNTTKNVNNNKN 494
>gi|114814|sp|P14164|BAF1_YEAST TRANSCRIPTION FACTOR BAF1 (ARS BINDING FACTOR 1) (PROTEIN ABF1)
(BIDIRECTIONALLY ACTING FACTOR) (SFB-B) (DNA REPLICATION
ENHANCER-BINDING PROTEIN OBF1) >gi|58415|emb|CAA35966|
(X51654) ARS-binding protein 1 [Saccharomyces
cerevisiae] >gi|170968 (M29067) ARS binding factor 1
[Saccharomyces cerevisiae] >gi|172058 (M63578) OBF1
[Saccharomyces cerevisiae] >gi|226720|prf||1604243A ARS
binding factor I [Saccharomyces cerevisiae]
Length = 731
Score = 36.4 bits (82), Expect = 0.23
Identities = 38/143 (26%), Positives = 64/143 (44%), Gaps = 9/143 (6%)
Query: 572 KEDASLASALKKDIPVSSGDSHKKDLPLSGGISYSGXNIPKKCLTAIVEENTEYDGLVKV 393
+++ SL + IP + K D L + S + K ++ VEE+ D L++
Sbjct: 343 EDNLSLGKFILTKIPKILQNDLKFDQILESSYNNSNHTVSKFKVSHYVEESGLLDILMQR 402
Query: 392 YPTTTSP---KLLSMMTYFDNSAKPGFVTTKKKVG-FKDISNSGYTYGKNKKEDGWQYVM 225
Y T +LLS + + K FV KKK+G + D+ S + N +
Sbjct: 403 YGLTAEDFEKRLLSQIARRITTYKARFVLKKKKMGEYNDLQPSSSSNNNNNNDGELSGTN 462
Query: 224 SRQKSID-----EIKSSAKTLNPWGRLKSSEN 144
R SID EI S+ + N + +++N
Sbjct: 463 LRSNSIDYAKHQEISSAGTSSNTTKNVNNNKN 494
>gi|320637|pir||S29870 DNA-binding protein BAF1 - yeast (Saccharomyces cerevisiae)
>gi|486187|emb|CAA81951| (Z28111) ORF YKL112w
[Saccharomyces cerevisiae] >gi|1680183|gb||S93804_2
(S93804) ARS-binding factor I [Saccharomyces cerevisiae]
Length = 731
Score = 36.4 bits (82), Expect = 0.23
Identities = 38/143 (26%), Positives = 64/143 (44%), Gaps = 9/143 (6%)
Query: 572 KEDASLASALKKDIPVSSGDSHKKDLPLSGGISYSGXNIPKKCLTAIVEENTEYDGLVKV 393
+++ SL + IP + K D L + S + K ++ VEE+ D L++
Sbjct: 343 EDNLSLGKFILTKIPKILQNDLKFDQILESSYNNSNHTVSKFKVSHYVEESGLLDILMQR 402
Query: 392 YPTTTSP---KLLSMMTYFDNSAKPGFVTTKKKVG-FKDISNSGYTYGKNKKEDGWQYVM 225
Y T +LLS + + K FV KKK+G + D+ S + N +
Sbjct: 403 YGLTAEDFEKRLLSQIARRITTYKARFVLKKKKMGEYNDLQPSSSSNNNNNNDGELSGTN 462
Query: 224 SRQKSID-----EIKSSAKTLNPWGRLKSSEN 144
R SID EI S+ + N + +++N
Sbjct: 463 LRSNSIDYAKHQEISSAGTSSNTTKNVNNNKN 494
>gi|3822238|gb|AAC70189|AAC70189 (AF081810) mucin-like protein [Lymantria dispar
nucleopolyhedrovirus]
Length = 1029
Score = 32.1 bits (71), Expect = 4.5
Identities = 49/204 (24%), Positives = 73/204 (35%), Gaps = 4/204 (1%)
Query: 665 TVCEKSKDVESDKIXXXKYYKLPVNDLDGEYKEDASLASALKKDIPVSSGDSHKKDLPLS 486
TV +KS+ +DK + K P + D K ++++ K + S D P
Sbjct: 279 TVSDKSRSPSADKHGSDRA-KSPKSGCDRP-KSSSAVSGKPKPEAKSKSSC----DKPTG 332
Query: 485 GGISYSGXNIPKKCLTAIVEENTEYDGLVKVYPTTTSPKLLSMMTYFDNSAKPGFVTTKK 306
G+S SG + PK + + VK PK S T D + V K
Sbjct: 333 PGVSTSGYDKPKSSSADKPSDKAKSSSTVK-------PKSSSADTPSDKAKSSSTVKPK- 384
Query: 305 KVGFKDISNSGYTYGKNKKEDGWQYVMSRQKSIDEIKSSAKTLNPWGRLKSSENPFETIK 126
S+S K K + KS +K + P + KSS T+K
Sbjct: 385 -------SSSDKPSDKAKSSSSSDKPCDKAKSSSTVKPKSSPDKPCDKAKSS----STVK 433
Query: 125 PL----KTPSPSENSPLESANPFKTTDP 54
P K+P + P + A T P
Sbjct: 434 PKSSSDKSPDKLSDKPSDKAKSSSTVKP 461
>gi|3024085|sp|Q28824|KMLS_BOVIN MYOSIN LIGHT CHAIN KINASE, SMOOTH MUSCLE (MLCK) (CONTAINS: TELOKIN)
>gi|484362|pir||JN0583 myosin-light-chain kinase (EC
2.7.1.117) 155K protein - bovine >gi|298639|bbs|127479
(S57131) 155 kda myosin light chain kinase homolog
[cattle, stomach, Peptide, 1176 aa] [Bos taurus]
Length = 1176
Score = 31.7 bits (70), Expect = 5.9
Identities = 18/45 (40%), Positives = 24/45 (53%), Gaps = 3/45 (6%)
Query: 188 AKTLNPWGRLKSSENP--FETIKPLKTPSPSEN-SPLESANPFKTTDP 54
A+T P G K +E P +KP +TP P N P E+ P +T P
Sbjct: 146 AETPKPLGSTKPAETPKPLGNVKPAETPKPLGNIKPTETPKPLGSTKP 193
Score = 31.3 bits (69), Expect = 7.7
Identities = 17/45 (37%), Positives = 24/45 (52%), Gaps = 3/45 (6%)
Query: 188 AKTLNPWGRLKSSENP--FETIKPLKTPSPSENS-PLESANPFKTTDP 54
A+T P G +K +E P +KP +TP P N+ P E+ P P
Sbjct: 218 AETPKPLGNVKPAETPKPLGNVKPAETPKPVSNAKPAETLKPVGNAKP 265
>gi|3914889|sp|O51453|RS13_BORBU 30S RIBOSOMAL PROTEIN S13 >gi|2688391 (AE001152) ribosomal protein
S13 (rpsM) [Borrelia burgdorferi]
Length = 125
Score = 31.3 bits (69), Expect = 7.7
Identities = 25/69 (36%), Positives = 40/69 (57%), Gaps = 10/69 (14%)
Query: 689 GMENTRYETVCEKS--------KDVESDKIXXXKYYKLPVNDLDGEYKEDASLASALKK- 537
G+ TR VC KS KD+++D++ + K+ +D E K + +A ++K+
Sbjct: 24 GIGRTRALEVCNKSSISPSKIAKDLDNDEVNRLR--KVIESDYIVEGKLRSEVAMSIKRL 81
Query: 536 -DIPVSSGDSHKKDLPLSG 483
DI G H+K LPL G
Sbjct: 82 MDIACYRGVRHRKGLPLRG 100
Database: Non-redundant GenBank CDS
translations+PDB+SwissProt+SPupdate+PIR
Posted date: Apr 17, 1999 5:33 AM
Number of letters in database: 113,023,754
Number of sequences in database: 369,800
Lambda K H
0.318 0.135 0.00
Gapped
Lambda K H
0.270 0.0470 4.94e-324
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 130420830
Number of Sequences: 369800
Number of extensions: 2435935
Number of successful extensions: 7210
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 7201
Number of HSP's gapped (non-prelim): 18
length of query: 247
length of database: 113023754
effective HSP length: 53
effective length of query: 194
effective length of database: 93424354
effective search space: 18124324676
effective search space used: 18124324676
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 68 (30.9 bits)