BLASTX 2.0.8 [Jan-05-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 14-12R
(662 letters)
Database: Non-redundant GenBank CDS
translations+PDB+SwissProt+SPupdate+PIR
368,476 sequences; 112,640,273 total letters
Searching...................................................done
Score E
Sequences producing significant alignments: (bits) Value
gi|1203939 (U49830) Similar to protein tyrosine phosphatase [Cae... 39 0.039
gi|3413|emb|CAA34421| (X16385) Baf1 protein (AA 1-731) [Saccharo... 36 0.20
gi|320637|pir||S29870 DNA-binding protein BAF1 - yeast (Saccharo... 36 0.20
gi|114814|sp|P14164|BAF1_YEAST TRANSCRIPTION FACTOR BAF1 (ARS BI... 36 0.20
gi|3024085|sp|Q28824|KMLS_BOVIN MYOSIN LIGHT CHAIN KINASE, SMOOT... 32 5.0
gi|2648297 (AE000951) conserved hypothetical protein [Archaeoglo... 31 8.6
gi|120776|sp|Q00422|GABA_MOUSE GA BINDING PROTEIN ALPHA CHAIN (G... 31 8.6
gi|1944199|dbj|BAA19658| (AB002817) dihydroflavonol 4-reductase ... 31 8.6
>gi|1203939 (U49830) Similar to protein tyrosine phosphatase [Caenorhabditis
elegans]
Length = 815
Score = 38.7 bits (88), Expect = 0.039
Identities = 43/129 (33%), Positives = 61/129 (46%), Gaps = 17/129 (13%)
Query: 446 CLTAIVEENTEYDGLVKVYPTTTSPKLLSMMTYFDNS--AKPGFVTTKKKVGFKDISNSG 273
C T + NT D +VY T P+ T DN+ +K G +KKK S
Sbjct: 338 CATLTFDHNTPGDHFERVYVNITLPE----KTGQDNNQGSKKGQTDSKKKD-----QGSR 388
Query: 272 YTYGKNKKEDGWQYVMSRQKS-------------IDEIKSSAKTLNP--WGRLKSSENPF 138
G +KK++ + R+ S I KS +P +GR K+ EN
Sbjct: 389 KARGNSKKKNHSSKKVVRKTSKKKKNAASPNAQLIPVAKSPGNDKSPAGFGRKKAGENLN 448
Query: 137 ETIKPLKTPSPSENSPLESANPFKTT 60
E +K L+ P+P LE N +KTT
Sbjct: 449 EVMKHLRAPTPRAQQELEKTN-WKTT 473
>gi|3413|emb|CAA34421| (X16385) Baf1 protein (AA 1-731) [Saccharomyces cerevisiae]
Length = 731
Score = 36.4 bits (82), Expect = 0.20
Identities = 38/143 (26%), Positives = 64/143 (44%), Gaps = 9/143 (6%)
Query: 572 KEDASLASALKKDIPVSSGDSHKKDLPLSGGISYSGXNIPKKCLTAIVEENTEYDGLVKV 393
+++ SL + IP + K D L + S + K ++ VEE+ D L++
Sbjct: 343 EDNLSLGKFILTKIPKILQNDLKFDQILESSYNNSNHTVSKFKVSHYVEESGLLDILMQR 402
Query: 392 YPTTTSP---KLLSMMTYFDNSAKPGFVTTKKKVG-FKDISNSGYTYGKNKKEDGWQYVM 225
Y T +LLS + + K FV KKK+G + D+ S + N +
Sbjct: 403 YGLTAEDFEKRLLSQIARRITTYKARFVLKKKKMGEYNDLQPSSSSNNNNNNDGELSGTN 462
Query: 224 SRQKSID-----EIKSSAKTLNPWGRLKSSEN 144
R SID EI S+ + N + +++N
Sbjct: 463 LRSNSIDYAKHQEISSAGTSSNTTKNVNNNKN 494
>gi|320637|pir||S29870 DNA-binding protein BAF1 - yeast (Saccharomyces cerevisiae)
>gi|486187|emb|CAA81951| (Z28111) ORF YKL112w
[Saccharomyces cerevisiae] >gi|1680183|gb||S93804_2
(S93804) ARS-binding factor I [Saccharomyces cerevisiae]
Length = 731
Score = 36.4 bits (82), Expect = 0.20
Identities = 38/143 (26%), Positives = 64/143 (44%), Gaps = 9/143 (6%)
Query: 572 KEDASLASALKKDIPVSSGDSHKKDLPLSGGISYSGXNIPKKCLTAIVEENTEYDGLVKV 393
+++ SL + IP + K D L + S + K ++ VEE+ D L++
Sbjct: 343 EDNLSLGKFILTKIPKILQNDLKFDQILESSYNNSNHTVSKFKVSHYVEESGLLDILMQR 402
Query: 392 YPTTTSP---KLLSMMTYFDNSAKPGFVTTKKKVG-FKDISNSGYTYGKNKKEDGWQYVM 225
Y T +LLS + + K FV KKK+G + D+ S + N +
Sbjct: 403 YGLTAEDFEKRLLSQIARRITTYKARFVLKKKKMGEYNDLQPSSSSNNNNNNDGELSGTN 462
Query: 224 SRQKSID-----EIKSSAKTLNPWGRLKSSEN 144
R SID EI S+ + N + +++N
Sbjct: 463 LRSNSIDYAKHQEISSAGTSSNTTKNVNNNKN 494
>gi|114814|sp|P14164|BAF1_YEAST TRANSCRIPTION FACTOR BAF1 (ARS BINDING FACTOR 1) (PROTEIN ABF1)
(BIDIRECTIONALLY ACTING FACTOR) (SFB-B) (DNA REPLICATION
ENHANCER-BINDING PROTEIN OBF1) >gi|58415|emb|CAA35966|
(X51654) ARS-binding protein 1 [Saccharomyces
cerevisiae] >gi|170968 (M29067) ARS binding factor 1
[Saccharomyces cerevisiae] >gi|172058 (M63578) OBF1
[Saccharomyces cerevisiae] >gi|226720|prf||1604243A ARS
binding factor I [Saccharomyces cerevisiae]
Length = 731
Score = 36.4 bits (82), Expect = 0.20
Identities = 38/143 (26%), Positives = 64/143 (44%), Gaps = 9/143 (6%)
Query: 572 KEDASLASALKKDIPVSSGDSHKKDLPLSGGISYSGXNIPKKCLTAIVEENTEYDGLVKV 393
+++ SL + IP + K D L + S + K ++ VEE+ D L++
Sbjct: 343 EDNLSLGKFILTKIPKILQNDLKFDQILESSYNNSNHTVSKFKVSHYVEESGLLDILMQR 402
Query: 392 YPTTTSP---KLLSMMTYFDNSAKPGFVTTKKKVG-FKDISNSGYTYGKNKKEDGWQYVM 225
Y T +LLS + + K FV KKK+G + D+ S + N +
Sbjct: 403 YGLTAEDFEKRLLSQIARRITTYKARFVLKKKKMGEYNDLQPSSSSNNNNNNDGELSGTN 462
Query: 224 SRQKSID-----EIKSSAKTLNPWGRLKSSEN 144
R SID EI S+ + N + +++N
Sbjct: 463 LRSNSIDYAKHQEISSAGTSSNTTKNVNNNKN 494
>gi|3024085|sp|Q28824|KMLS_BOVIN MYOSIN LIGHT CHAIN KINASE, SMOOTH MUSCLE (MLCK) (CONTAINS: TELOKIN)
>gi|484362|pir||JN0583 myosin-light-chain kinase (EC
2.7.1.117) 155K protein - bovine >gi|298639|bbs|127479
(S57131) 155 kda myosin light chain kinase homolog
[cattle, stomach, Peptide, 1176 aa] [Bos taurus]
Length = 1176
Score = 31.7 bits (70), Expect = 5.0
Identities = 18/45 (40%), Positives = 24/45 (53%), Gaps = 3/45 (6%)
Query: 188 AKTLNPWGRLKSSENP--FETIKPLKTPSPSEN-SPLESANPFKTTDP 54
A+T P G K +E P +KP +TP P N P E+ P +T P
Sbjct: 146 AETPKPLGSTKPAETPKPLGNVKPAETPKPLGNIKPTETPKPLGSTKP 193
Score = 31.3 bits (69), Expect = 6.6
Identities = 17/45 (37%), Positives = 24/45 (52%), Gaps = 3/45 (6%)
Query: 188 AKTLNPWGRLKSSENP--FETIKPLKTPSPSENS-PLESANPFKTTDP 54
A+T P G +K +E P +KP +TP P N+ P E+ P P
Sbjct: 218 AETPKPLGNVKPAETPKPLGNVKPAETPKPVSNAKPAETLKPVGNAKP 265
>gi|2648297 (AE000951) conserved hypothetical protein [Archaeoglobus fulgidus]
Length = 322
Score = 30.9 bits (68), Expect = 8.6
Identities = 19/58 (32%), Positives = 29/58 (49%), Gaps = 14/58 (24%)
Query: 482 GISYSGXNIPKKCLTAIVEENTEYDGLVKVYPTTTSPK--------------LLSMMTYF 345
G S G +PK V NT YD ++K T+P+ L +++YF
Sbjct: 147 GESLGGYTVPKNSFALGVISNTIYDVILKTAGVNTTPEYAGLMSVENMEARGLTELISYF 206
Query: 344 DNSAKPGFVTTK 309
+ PG + K
Sbjct: 207 GTTLSPGLLLLK 218
>gi|120776|sp|Q00422|GABA_MOUSE GA BINDING PROTEIN ALPHA CHAIN (GABP-ALPHA SUBUNIT)
>gi|109846|pir||A40858 GA-binding protein alpha chain -
mouse >gi|193383 (M74515) GA binding protein [Mus
musculus]
Length = 454
Score = 30.9 bits (68), Expect = 8.6
Identities = 21/87 (24%), Positives = 41/87 (46%), Gaps = 4/87 (4%)
Query: 485 GGISYSGXNIPKKCLTAIVEENTE----YDGLVKVYPTTTSPKLLSMMTYFDNSAKPGFV 318
G I +S + +K + A E+ D V++ P + P + + ++SAK V
Sbjct: 237 GEILWSHLELLRKYVLASQEQQMNEIVTIDQPVQIIPASVPPATPTTIKVINSSAKAAKV 296
Query: 317 TTKKKVGFKDISNSGYTYGKNKKEDGWQYVM 225
++ +D S+ G G N + WQ+++
Sbjct: 297 QRSPRISGEDRSSPGNRTGNNGQIQLWQFLL 327
>gi|1944199|dbj|BAA19658| (AB002817) dihydroflavonol 4-reductase [Perilla frutescens]
Length = 456
Score = 30.9 bits (68), Expect = 8.6
Identities = 21/79 (26%), Positives = 37/79 (46%)
Query: 410 DGLVKVYPTTTSPKLLSMMTYFDNSAKPGFVTTKKKVGFKDISNSGYTYGKNKKEDGWQY 231
DG++ + + T K + + F NSA V +K + + + S + +KK GW Y
Sbjct: 112 DGMLSIMRSCTKAKTVKKLI-FTNSAGTLNVEEHQKPVYNEANWSDLDFIYSKKMTGWMY 170
Query: 230 VMSRQKSIDEIKSSAKTLN 174
+S+ + E +AK N
Sbjct: 171 FVSKILAEKEAMKAAKESN 189
Database: Non-redundant GenBank CDS
translations+PDB+SwissProt+SPupdate+PIR
Posted date: Apr 12, 1999 12:54 PM
Number of letters in database: 112,640,273
Number of sequences in database: 368,476
Lambda K H
0.318 0.135 0.00
Gapped
Lambda K H
0.270 0.0470 4.94e-324
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 108244659
Number of Sequences: 368476
Number of extensions: 2039663
Number of successful extensions: 5686
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 5678
Number of HSP's gapped (non-prelim): 16
length of query: 220
length of database: 112640273
effective HSP length: 53
effective length of query: 167
effective length of database: 93111045
effective search space: 15549544515
effective search space used: 15549544515
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 68 (30.9 bits)