BLASTX 2.0.8 [Jan-05-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 14-10R
(549 letters)
Database: Non-redundant GenBank CDS
translations+PDB+SwissProt+SPupdate+PIR
368,476 sequences; 112,640,273 total letters
Searching...................................................done
Score E
Sequences producing significant alignments: (bits) Value
gi|3941720 (AF087021) tyrosyl-tRNA synthetase; tyrosine--tRNA li... 91 6e-18
gi|4128224 (AF096709) tyrosyl-tRNA synthetase [Drosophila virilis] 89 2e-17
gi|1711657|sp|P54577|SYY_HUMAN TYROSYL-TRNA SYNTHETASE (TYROSYL-... 89 3e-17
gi|2665519 (U89436) tyrosyl-tRNA synthetase [Homo sapiens] >gi|4... 89 3e-17
gi|2501079|sp|Q29465|SYY_BOVIN TYROSYL-TRNA SYNTHETASE (TYROSYL-... 80 1e-14
gi|3183174|sp|O14055|SYYC_SCHPO TYROSYL-TRNA SYNTHETASE, CYTOPLA... 74 7e-13
gi|549037|sp|P36421|SYYC_YEAST TYROSYL-TRNA SYNTHETASE, CYTOPLAS... 69 2e-11
gi|1584013|prf||2122232A Tyr-tRNA synthetase [Pneumocystis carinii] 69 2e-11
gi|3334364|sp|O27795|SYY_METTH TYROSYL-TRNA SYNTHETASE (TYROSINE... 53 1e-06
gi|2501077|sp|Q57834|SYY_METJA TYROSYL-TRNA SYNTHETASE (TYROSINE... 46 1e-04
gi|1841468|emb|CAA71881| (Y10990) Tyrosyl-tRNA synthetase [Nicot... 41 0.005
gi|1707012 (U78721) tyrosyl-tRNA synthetase isolog [Arabidopsis ... 34 1.0
gi|2689557 (U93688) orf10 [Staphylococcus aureus] 31 5.1
>gi|3941720 (AF087021) tyrosyl-tRNA synthetase; tyrosine--tRNA ligase [Bos
taurus]
Length = 528
Score = 90.9 bits (222), Expect = 6e-18
Identities = 44/79 (55%), Positives = 59/79 (73%)
Query: 355 SMSKDENXXLISQNL*EVLGKDKLMDILKMRDLKLXRGTATTGKSHIAYFIRLLKIRNLL 176
S+S +E LI++NL EVLG++KL +ILK R+LK+ GTATTGK H+AYF+ + KI + L
Sbjct: 4 SLSPEEKLSLITRNLQEVLGEEKLKEILKERELKVYWGTATTGKPHVAYFVPMSKIADFL 63
Query: 175 AAGCKAKILLAEXHALLDN 119
AGC+ IL A+ HA LDN
Sbjct: 64 KAGCEVTILFADLHAYLDN 82
>gi|4128224 (AF096709) tyrosyl-tRNA synthetase [Drosophila virilis]
Length = 237
Score = 89.3 bits (218), Expect = 2e-17
Identities = 43/79 (54%), Positives = 57/79 (71%)
Query: 355 SMSKDENXXLISQNL*EVLGKDKLMDILKMRDLKLXRGTATTGKSHIAYFIRLLKIRNLL 176
++S DE LI++NL E LG+DKL +L+ RDLK+ GTATTGK H+AYF+ + KI + L
Sbjct: 5 TLSADEKKQLITRNLQETLGEDKLNKVLQERDLKIYWGTATTGKPHVAYFVPMSKIADFL 64
Query: 175 AAGCKAKILLAEXHALLDN 119
AGC+ IL A+ HA LDN
Sbjct: 65 KAGCEVTILFADLHAYLDN 83
>gi|1711657|sp|P54577|SYY_HUMAN TYROSYL-TRNA SYNTHETASE (TYROSYL--TRNA LIGASE) (TYRRS) >gi|1184699
(U40714) tyrosyl-tRNA synthetase [Homo sapiens]
Length = 388
Score = 88.5 bits (216), Expect = 3e-17
Identities = 43/77 (55%), Positives = 57/77 (73%)
Query: 349 SKDENXXLISQNL*EVLGKDKLMDILKMRDLKLXRGTATTGKSHIAYFIRLLKIRNLLAA 170
S +E LI++NL EVLG++KL +ILK R+LK+ GTATTGK H+AYF+ + KI + L A
Sbjct: 6 SPEEKLHLITRNLQEVLGEEKLKEILKERELKIYWGTATTGKPHVAYFVPMSKIADFLKA 65
Query: 169 GCKAKILLAEXHALLDN 119
GC+ IL A+ HA LDN
Sbjct: 66 GCEVTILFADLHAYLDN 82
>gi|2665519 (U89436) tyrosyl-tRNA synthetase [Homo sapiens]
>gi|4507947|ref|NP_003671.1|pYARS| tyrosyl-tRNA
synthetase; tyrosyl tRNA ligase
Length = 528
Score = 88.5 bits (216), Expect = 3e-17
Identities = 43/77 (55%), Positives = 57/77 (73%)
Query: 349 SKDENXXLISQNL*EVLGKDKLMDILKMRDLKLXRGTATTGKSHIAYFIRLLKIRNLLAA 170
S +E LI++NL EVLG++KL +ILK R+LK+ GTATTGK H+AYF+ + KI + L A
Sbjct: 6 SPEEKLHLITRNLQEVLGEEKLKEILKERELKIYWGTATTGKPHVAYFVPMSKIADFLKA 65
Query: 169 GCKAKILLAEXHALLDN 119
GC+ IL A+ HA LDN
Sbjct: 66 GCEVTILFADLHAYLDN 82
>gi|2501079|sp|Q29465|SYY_BOVIN TYROSYL-TRNA SYNTHETASE (TYROSYL--TRNA LIGASE) (TYRRS)
>gi|1212808|emb|CAA65245| (X96373) tyrosyl-tRNA
synthetase [Bos taurus]
Length = 196
Score = 79.6 bits (193), Expect = 1e-14
Identities = 38/66 (57%), Positives = 49/66 (73%)
Query: 316 NL*EVLGKDKLMDILKMRDLKLXRGTATTGKSHIAYFIRLLKIRNLLAAGCKAKILLAEX 137
NL E LG++KL +ILK R+LK+ GTATTGK H+AYF+ + KI + L AGC+ IL A+
Sbjct: 1 NLQEALGEEKLKEILKERELKVYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADL 60
Query: 136 HALLDN 119
HA LDN
Sbjct: 61 HAYLDN 66
>gi|3183174|sp|O14055|SYYC_SCHPO TYROSYL-TRNA SYNTHETASE, CYTOPLASMIC (TYROSYL--TRNA LIGASE) (TYRRS)
>gi|3451306|emb|CAA20443| (AL031324) tyrosyl-trna
synthetase cytoplasmic EC 6.1.1.1 [Schizosaccharomyces
pombe]
Length = 401
Score = 74.1 bits (179), Expect = 7e-13
Identities = 38/89 (42%), Positives = 56/89 (62%), Gaps = 6/89 (6%)
Query: 358 VSMSKDENXXLISQNL*EVLGKDKLMDILKMRDLKLXRGTATTGKSHIAYFIRLLKIRNL 179
+S++ DE LI++NL EVLG + +IL RD+ L G+A TG+ H YF+ ++K+ +
Sbjct: 1 MSLTPDEKYELITRNLQEVLGAKMIREILNERDISLYWGSAPTGRPHCGYFVPMMKLADF 60
Query: 178 LAAGCKAKILLAEXHALLDNKN------QHRANRF 92
L AG +LLA+ HA LDN QHR +
Sbjct: 61 LKAGVHVTVLLADVHAFLDNMKAPMELVQHRVRYY 95
>gi|549037|sp|P36421|SYYC_YEAST TYROSYL-TRNA SYNTHETASE, CYTOPLASMIC (TYROSYL--TRNA LIGASE) (TYRRS)
>gi|539380|pir||A45999 tyrosine--tRNA ligase (EC
6.1.1.1) - yeast (Saccharomyces cerevisiae) >gi|173120
(L12221) tyrosyl-tRNA synthetase [Saccharomyces
cerevisiae] >gi|1323328|emb|CAA97211| (Z72970) ORF
YGR185c [Saccharomyces cerevisiae]
>gi|1430951|emb|CAA67529| (X99074) G7522 [Saccharomyces
cerevisiae]
Length = 394
Score = 69.1 bits (166), Expect = 2e-11
Identities = 36/83 (43%), Positives = 53/83 (63%), Gaps = 2/83 (2%)
Query: 367 SLLVSMSKDENXXLISQNL*EVLGKDKLMDILKM--RDLKLXRGTATTGKSHIAYFIRLL 194
S ++ +E LI++NL EVL + D+L++ R LKL GTA TG+ H YF+ +
Sbjct: 2 SSAATVDPNEAFGLITKNLQEVLNPQIIKDVLEVQKRHLKLYWGTAPTGRPHCGYFVPMT 61
Query: 193 KIRNLLAAGCKAKILLAEXHALLDN 119
K+ + L AGC+ +LLA+ HA LDN
Sbjct: 62 KLADFLKAGCEVTVLLADLHAFLDN 86
>gi|1584013|prf||2122232A Tyr-tRNA synthetase [Pneumocystis carinii]
Length = 370
Score = 69.1 bits (166), Expect = 2e-11
Identities = 35/70 (50%), Positives = 47/70 (67%)
Query: 328 LISQNL*EVLGKDKLMDILKMRDLKLXRGTATTGKSHIAYFIRLLKIRNLLAAGCKAKIL 149
LI++ L EVLG ++L IL+ RDLKL GT+ TGK H YF+ ++KI + L A + IL
Sbjct: 14 LITRGLQEVLGAERLRKILEERDLKLYWGTSPTGKPHCGYFVPMIKIADFLKAEVEVTIL 73
Query: 148 LAEXHALLDN 119
A+ HA LDN
Sbjct: 74 FADIHAFLDN 83
>gi|3334364|sp|O27795|SYY_METTH TYROSYL-TRNA SYNTHETASE (TYROSINE--TRNA LIGASE) (TYRRS) >gi|2622897
(AE000932) tyrosyl-tRNA synthetase [Methanobacterium
thermoautotrophicum]
Length = 319
Score = 53.1 bits (125), Expect = 1e-06
Identities = 26/76 (34%), Positives = 48/76 (62%)
Query: 343 DENXXLISQNL*EVLGKDKLMDILKMRDLKLXRGTATTGKSHIAYFIRLLKIRNLLAAGC 164
D++ I++++ EV+ ++LMD+LK + + G +G+ H+ + + + K+R L AG
Sbjct: 2 DDSLKTITRDVLEVITPEELMDVLKKDEPVVYTGYEPSGRIHLGHALTVRKLRELQDAGF 61
Query: 163 KAKILLAEXHALLDNK 116
+ KILLA+ HA L+ K
Sbjct: 62 RVKILLADYHAYLNGK 77
>gi|2501077|sp|Q57834|SYY_METJA TYROSYL-TRNA SYNTHETASE (TYROSINE--TRNA LIGASE) (TYRRS)
>gi|2129341|pir||E64348 tyrosine--tRNA ligase (EC
6.1.1.1) - Methanococcus jannaschii >gi|1591094 (U67491)
tyrosyl-tRNA synthetase (trS) [Methanococcus jannaschii]
Length = 306
Score = 46.5 bits (108), Expect = 1e-04
Identities = 22/73 (30%), Positives = 44/73 (60%)
Query: 328 LISQNL*EVLGKDKLMDILKMRDLKLXRGTATTGKSHIAYFIRLLKIRNLLAAGCKAKIL 149
+I +N E++ +++L ++LK + G +GK H+ +++++ K+ +L AG IL
Sbjct: 6 MIKRNTSEIISEEELREVLKKDEKSAYIGFEPSGKIHLGHYLQIKKMIDLQNAGFDIIIL 65
Query: 148 LAEXHALLDNKNQ 110
LA+ HA L+ K +
Sbjct: 66 LADLHAYLNQKGE 78
>gi|1841468|emb|CAA71881| (Y10990) Tyrosyl-tRNA synthetase [Nicotiana tabacum]
Length = 408
Score = 41.4 bits (95), Expect = 0.005
Identities = 26/79 (32%), Positives = 45/79 (56%), Gaps = 2/79 (2%)
Query: 352 MSKDENXXLISQNL*EVLGKDKLMDILKMRDLKL-XRGTATTGKSHIAY-FIRLLKIRNL 179
MS +E ++ E + +D+LM++L + + G +G+ HIA ++ L + L
Sbjct: 58 MSLEEKFKIVRSIGEECIQEDELMNLLAKKPQPVCYDGFEPSGRMHIAQGVLKALNVNKL 117
Query: 178 LAAGCKAKILLAEXHALLDNK 116
+AGCK KI +A+ A L+NK
Sbjct: 118 TSAGCKVKIWIADWFAQLNNK 138
>gi|1707012 (U78721) tyrosyl-tRNA synthetase isolog [Arabidopsis thaliana]
Length = 460
Score = 33.6 bits (75), Expect = 1.0
Identities = 18/64 (28%), Positives = 39/64 (60%), Gaps = 2/64 (3%)
Query: 307 EVLGKDKLMDILKMRDLKL-XRGTATTGKSHIAYFI-RLLKIRNLLAAGCKAKILLAEXH 134
E + +++L ++L + + G +G+ HIA + +++ + + +AGC+ KI +A+
Sbjct: 18 ECIQEEELKNLLAKKAAPICYDGFEPSGRMHIAQGVMKVINVNKMTSAGCRVKIWIADWF 77
Query: 133 ALLDNK 116
A L+NK
Sbjct: 78 AQLNNK 83
>gi|2689557 (U93688) orf10 [Staphylococcus aureus]
Length = 89
Score = 31.3 bits (69), Expect = 5.1
Identities = 15/40 (37%), Positives = 29/40 (72%), Gaps = 1/40 (2%)
Query: 493 NLFIAPQLLSRG-YMFGYNLSLF*THVYIFCLLMKLLFIEI 374
N F+ +LL +G ++ +SLF T++YIFC+L K++ +++
Sbjct: 2 NFFL--RLLPQGLHLICLTVSLFTTYIYIFCILCKIISVKL 40
Database: Non-redundant GenBank CDS
translations+PDB+SwissProt+SPupdate+PIR
Posted date: Apr 12, 1999 12:54 PM
Number of letters in database: 112,640,273
Number of sequences in database: 368,476
Lambda K H
0.318 0.135 0.00
Gapped
Lambda K H
0.270 0.0470 4.94e-324
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 95341029
Number of Sequences: 368476
Number of extensions: 1721761
Number of successful extensions: 4267
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 4255
Number of HSP's gapped (non-prelim): 13
length of query: 183
length of database: 112640273
effective HSP length: 52
effective length of query: 130
effective length of database: 93479521
effective search space: 12152337730
effective search space used: 12152337730
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 67 (30.5 bits)