BLASTX 2.0.8 [Jan-05-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 12VII-7R
(818 letters)
Database: Non-redundant GenBank CDS
translations+PDB+SwissProt+SPupdate+PIR
368,476 sequences; 112,640,273 total letters
Searching...................................................done
Score E
Sequences producing significant alignments: (bits) Value
gi|140550|sp|P09975|YCF2_MARPO HYPOTHETICAL 259 KD PROTEIN (ORF ... 36 0.26
gi|3845252 (AE001412) hypothetical protein [Plasmodium falciparum] 34 1.3
gi|4493950|emb|CAB38986| (AL034556) predicted using hexExon; MAL... 34 1.3
gi|2827016 (AF008210) DNA gyrase subunit B [Buchnera aphidicola] 34 1.7
gi|1088430|dbj|BAA04740| (D21196) ORF1740; The deduced amino aci... 34 1.7
gi|3334405|sp|O23948|VATE_GOSHI VACUOLAR ATP SYNTHASE SUBUNIT E ... 33 2.9
gi|4493933|emb|CAB38969| (AL034556) predicted using hexExon; MAL... 32 3.8
gi|2121278 (AF000270) unknown [Borrelia burgdorferi] 32 5.0
gi|1209828 (U45421) 2.9-1 ORF-D [Borrelia burgdorferi] 32 6.5
gi|1209846 (U45424) 2.9-4 ORF-D [Borrelia burgdorferi] 32 6.5
gi|84040|pir||E22845 hypothetical protein 4 - Trypanosoma brucei... 32 6.5
gi|2865294 (AF022236) Orf18 [Escherichia coli] 32 6.5
gi|1209863 (U45426) 2.9-6 ORF-D [Borrelia burgdorferi] 31 8.6
gi|4049717 (AF063866) ORF MSV156 hypothetical protein [Melanoplu... 31 8.6
>gi|140550|sp|P09975|YCF2_MARPO HYPOTHETICAL 259 KD PROTEIN (ORF 2136) >gi|81341|pir||A05037
hypothetical protein 2136 - liverwort (Marchantia
polymorpha) chloroplast >gi|11665|emb|CAA28078| (X04465)
ORF2136 [Marchantia polymorpha]
Length = 2136
Score = 36.4 bits (82), Expect = 0.26
Identities = 43/142 (30%), Positives = 71/142 (49%), Gaps = 10/142 (7%)
Query: 774 ISNQLSEYLKYLEIFLKDLINECTTHSRRYFEIKRLSEKKYH----NKIKNNFVTKIFYK 607
I N YL+ L+IFLKD N + EIK EKK + +++ N + K YK
Sbjct: 905 IKNNNLLYLRLLKIFLKDKRNFLLIN-----EIKSFIEKKNNLFIKSQLSNVLLVKNSYK 959
Query: 606 --KNLNSLKFIKKIEALAEYNLNRLKKTTKNIIY*TF*DVP**L*NYYE*QY----YRLL 445
N+ + F+K+ E E LN K+++ T+ +Y + Y ++LL
Sbjct: 960 FFDNIFNFHFLKQKEKNIEIILNNQNYFEKSLLKKTYLKNLNLNNSYSKFSYKIFIFQLL 1019
Query: 444 SGIYKNNYQ*YNFIING*SDIQLFFTNXKLHY 349
+ + KNNY+ + +I +L F + L+Y
Sbjct: 1020 NILNKNNYKTFQWI------SELIFYSKNLNY 1045
>gi|3845252 (AE001412) hypothetical protein [Plasmodium falciparum]
Length = 244
Score = 34.0 bits (76), Expect = 1.3
Identities = 27/100 (27%), Positives = 49/100 (49%), Gaps = 6/100 (6%)
Query: 813 NKKKLFECKSISDISNQLSEYLK---YLEIFLKDLINECTTHSRRYFEI---KRLSEKKY 652
NK FE ++ ++ +++ + L+ Y++ ++++ NEC Y + +R+S K
Sbjct: 117 NKIHKFEHDTLEELVHKMEQELETKMYIKNDIENIFNECINKKDEYLKDITQERISVFKE 176
Query: 651 HNKIKNNFVTKIFYKKNLNSLKFIKKIEALAEYNLNRLKKTTKNII 514
K +N L L I K E YN+N LKK N++
Sbjct: 177 RKKRQNQ----------LQKLLLIMKQENNKNYNINYLKKYESNLM 212
>gi|4493950|emb|CAB38986| (AL034556) predicted using hexExon; MAL3P5.4 (PFC0590c),
Hypothetical protein, len: 1828 aa [Plasmodium
falciparum]
Length = 1827
Score = 34.0 bits (76), Expect = 1.3
Identities = 19/62 (30%), Positives = 30/62 (47%)
Query: 768 NQLSEYLKYLEIFLKDLINECTTHSRRYFEIKRLSEKKYHNKIKNNFVTKIFYKKNLNSL 589
N + Y Y ++ + +N T Y + K+Y+ KIKN+ + K KK + SL
Sbjct: 71 NNNNSYFSYCNVYKNNDVNNNYTAYNYYINNPKNKLKEYYEKIKNHVIKK---KKKIFSL 127
Query: 588 KF 583
KF
Sbjct: 128 KF 129
>gi|2827016 (AF008210) DNA gyrase subunit B [Buchnera aphidicola]
Length = 803
Score = 33.6 bits (75), Expect = 1.7
Identities = 32/98 (32%), Positives = 49/98 (49%), Gaps = 14/98 (14%)
Query: 813 NKKKLFECKSISDISNQLSEYLKYLEIFLKDLINECTTHSRRYFEIKRLSEKKYHNKIKN 634
+K K E SI + S +LS Y + + LK+ E S+ YF I L EK +
Sbjct: 648 SKIKRNENDSIFEPSIKLSRYANHTQYDLKNDFLE----SKEYFLITNLGEKFKKFQENE 703
Query: 633 NFVTKIFYKKNLNSLK--------------FIKKIEALAEYNLNRLKKTTKN 520
NF+ K +N +K F+++ + L E N ++L TT N
Sbjct: 704 NFIEKGEKIYKINDIKNTLEWLIKETKRGFFVQRYKGLGEMNPDQLWNTTMN 755
>gi|1088430|dbj|BAA04740| (D21196) ORF1740; The deduced amino acid sequence from
region(11558..11779) is weakly homologous to the
C-terminus 58 amino acids of ribosomal protein S3
[Dictyostelium discoideum]
Length = 1740
Score = 33.6 bits (75), Expect = 1.7
Identities = 31/93 (33%), Positives = 46/93 (49%), Gaps = 5/93 (5%)
Query: 801 LFECKSISDISNQLSEYLKYLEIFLKDLINE-----CTTHSRRYFEIKRLSEKKYHNKIK 637
L E K I+D ++ LKYLE +L++L T H+R Y R KK KIK
Sbjct: 1304 LIERKGIADEPSE----LKYLEEYLQNLTTRLPAKYVTAHARNYRRTSRTVIKK-KQKIK 1358
Query: 636 NNFVTKIFYKKNLNSLKFIKKIEALAEYNLNRLKKTTK 523
+ K YK +S K ++KI ++ + + K K
Sbjct: 1359 PRNIRK--YKIKKSSRKVLEKIRSIYSIGVRKRFKYRK 1394
>gi|3334405|sp|O23948|VATE_GOSHI VACUOLAR ATP SYNTHASE SUBUNIT E (V-ATPASE E SUBUNIT) >gi|2267583
(AF009338) vacuolar H+-ATPase subunit E [Gossypium
hirsutum]
Length = 237
Score = 32.8 bits (73), Expect = 2.9
Identities = 23/84 (27%), Positives = 45/84 (53%), Gaps = 4/84 (4%)
Query: 786 SISDISNQLSEYLKYLEIFLKDLINECTTHSRRYFEIKRL----SEKKYHNKIKNNFVTK 619
S +D+S Q+ + ++++ ++ NE + + F I++L +EKK KI+
Sbjct: 2 SDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKK---KIRQE---- 54
Query: 618 IFYKKNLNSLKFIKKIEALAEYNLNRLK 535
Y+K ++ KKIE + N +R+K
Sbjct: 55 --YEKKEKQVEIRKKIEYSMQLNASRIK 80
>gi|4493933|emb|CAB38969| (AL034556) predicted using hexExon; MAL3P5.1 (PFC0575w),
Hypothetical protein, len: 645 aa [Plasmodium
falciparum]
Length = 644
Score = 32.5 bits (72), Expect = 3.8
Identities = 16/45 (35%), Positives = 25/45 (55%), Gaps = 1/45 (2%)
Query: 75 ICKK*FFVYCNIIVYGH-SQIFIIFLXYFCNFLKLSIYENICDEII 209
IC +F YC ++ + S + I F +FC FL +I E I +E +
Sbjct: 192 ICTLIYFTYCMCLLIKYISHLCIFFFFFFCFFLCYNILERIYEECV 237
>gi|2121278 (AF000270) unknown [Borrelia burgdorferi]
Length = 117
Score = 32.1 bits (71), Expect = 5.0
Identities = 25/78 (32%), Positives = 41/78 (52%), Gaps = 3/78 (3%)
Query: 789 KSISDISNQLSEYLKYLEIFL---KDLINECTTHSRRYFEIKRLSEKKYHNKIKNNFVTK 619
KS++ QLSEY+ YL FL K +N+ Y ++ L ++ +K+N K
Sbjct: 35 KSLAKYETQLSEYVMYLVTFLAKTKVKVNDPNYPEYPYPDLSTLKDEHSIPAVKHNI--K 92
Query: 618 IFYKKNLNSLKFIKKIEALAE 556
I+ L++IKK + +AE
Sbjct: 93 IY-------LEYIKKAKPIAE 106
>gi|1209828 (U45421) 2.9-1 ORF-D [Borrelia burgdorferi]
Length = 117
Score = 31.7 bits (70), Expect = 6.5
Identities = 25/83 (30%), Positives = 42/83 (50%), Gaps = 3/83 (3%)
Query: 789 KSISDISNQLSEYLKYLEIFL---KDLINECTTHSRRYFEIKRLSEKKYHNKIKNNFVTK 619
KS++ QLSEY+ YL FL K +N+ Y ++ L ++ +K+N
Sbjct: 35 KSLAKYETQLSEYVMYLVTFLAKTKVKVNDPNYPEYPYPDLSTLKDEHSITAVKHNI--N 92
Query: 618 IFYKKNLNSLKFIKKIEALAEYNLNR 541
I+ L++IKK + +AE N+
Sbjct: 93 IY-------LEYIKKTKPIAEKVYNK 111
>gi|1209846 (U45424) 2.9-4 ORF-D [Borrelia burgdorferi]
Length = 117
Score = 31.7 bits (70), Expect = 6.5
Identities = 25/83 (30%), Positives = 42/83 (50%), Gaps = 3/83 (3%)
Query: 789 KSISDISNQLSEYLKYLEIFL---KDLINECTTHSRRYFEIKRLSEKKYHNKIKNNFVTK 619
KS++ QLSEY+ YL FL K +N+ Y ++ L ++ +K+N
Sbjct: 35 KSLAKYETQLSEYVMYLVTFLAKTKVKVNDPNYPEYPYPDLSTLKDEHSITAVKHNI--N 92
Query: 618 IFYKKNLNSLKFIKKIEALAEYNLNR 541
I+ L++IKK + +AE N+
Sbjct: 93 IY-------LEYIKKTKPIAEKVYNK 111
>gi|84040|pir||E22845 hypothetical protein 4 - Trypanosoma brucei mitochondrion (SGC6)
Length = 445
Score = 31.7 bits (70), Expect = 6.5
Identities = 32/132 (24%), Positives = 63/132 (47%), Gaps = 6/132 (4%)
Query: 90 FFVYCNIIVYGHSQIFIIFLXYFCNFLKLSIYENICDEIINIQKFCKIRVHIHLIISAW* 269
+ ++C I++ + I F+ ++ F+KL ++++ +I + F + + L ++ +
Sbjct: 216 YILFCFILII----LLISFIYFYILFIKLLLFQSCTCVLIGLNSFAIVSLLFVLSVNNFC 271
Query: 270 RNFLVSIVAYN*X*YSFYLV*NEKSLNNVIXYL*KTVVYLINR***NYII-YK--- 437
FL+ I N Y FYL N + ++ L + Y +II + F +K
Sbjct: 272 FLFLIFISTKN---YIFYLYLNFHLIYSISLVLLIIIYYF-------FIIYNIFDFKYNE 321
Query: 438 --FLIIICNIAIHNNFII 485
FLI + NNF+I
Sbjct: 322 NYFLINFIFFSFFNNFLI 339
>gi|2865294 (AF022236) Orf18 [Escherichia coli]
Length = 176
Score = 31.7 bits (70), Expect = 6.5
Identities = 19/52 (36%), Positives = 29/52 (55%), Gaps = 2/52 (3%)
Query: 506 HSRMFP--NDYEIIMNSNITDYYQEFIKTTINNIILLSTVNQIYNCFSQIXNYI 351
H R+F +DY II N DY FIK N + ++ + Q CFS+I +++
Sbjct: 56 HHRVFAGIDDYSII-NFRGKDYAVRFIKDKDNYVYKVNKITQESGCFSRIFSFL 108
>gi|1209863 (U45426) 2.9-6 ORF-D [Borrelia burgdorferi]
Length = 117
Score = 31.3 bits (69), Expect = 8.6
Identities = 25/83 (30%), Positives = 41/83 (49%), Gaps = 3/83 (3%)
Query: 789 KSISDISNQLSEYLKYLEIFL---KDLINECTTHSRRYFEIKRLSEKKYHNKIKNNFVTK 619
KS++ QLSEY+ YL FL K +N+ Y ++ L ++ +K+N
Sbjct: 35 KSLAKYETQLSEYVMYLVTFLAKTKVKVNDPNYPEYPYPDLSTLKDEHSITAVKHNI--N 92
Query: 618 IFYKKNLNSLKFIKKIEALAEYNLNR 541
I+ L++IKK +AE N+
Sbjct: 93 IY-------LEYIKKTNPIAEKVYNK 111
>gi|4049717 (AF063866) ORF MSV156 hypothetical protein [Melanoplus sanguinipes
entomopoxvirus]
Length = 1127
Score = 31.3 bits (69), Expect = 8.6
Identities = 28/101 (27%), Positives = 48/101 (46%)
Query: 816 RNKKKLFECKSISDISNQLSEYLKYLEIFLKDLINECTTHSRRYFEIKRLSEKKYHNKIK 637
++K KLF+ I ++N ++E + I + +S R F+ K +E K + IK
Sbjct: 363 KSKIKLFD-NDIQKLNNDITEQ--------NNKITDFFNNSTRIFKEKLDTEYKKIDDIK 413
Query: 636 NNFVTKIFYKKNLNSLKFIKKIEALAEYNLNRLKKTTKNII 514
NN + K+ + KKI+ EY N++ K +II
Sbjct: 414 NNNLQKLE--------ESYKKIDEQTEYYKNKINKEYNDII 446
Database: Non-redundant GenBank CDS
translations+PDB+SwissProt+SPupdate+PIR
Posted date: Apr 12, 1999 12:54 PM
Number of letters in database: 112,640,273
Number of sequences in database: 368,476
Lambda K H
0.318 0.135 0.00
Gapped
Lambda K H
0.270 0.0470 4.94e-324
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 135505494
Number of Sequences: 368476
Number of extensions: 2565594
Number of successful extensions: 6924
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 14
Number of HSP's that attempted gapping in prelim test: 6921
Number of HSP's gapped (non-prelim): 19
length of query: 272
length of database: 112640273
effective HSP length: 54
effective length of query: 218
effective length of database: 92742569
effective search space: 20217880042
effective search space used: 20217880042
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 69 (31.3 bits)