BLASTX 2.0.8 [Jan-05-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 12VII-28F
(492 letters)
Database: Non-redundant GenBank CDS
translations+PDB+SwissProt+SPupdate+PIR
369,800 sequences; 113,023,754 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gi|130593|sp|P05400|POL_CERV ENZYMATIC POLYPROTEIN [CONTAINS: AS... 38 0.045
gi|2791289|emb|CAA04050| (AJ000387) protease [Drosophila melanog... 33 1.1
gi|2147955|pir||S68349 pol polyprotein - Tribolium freemani retr... 33 1.5
gi|2147954|pir||S68350 pol polyprotein - Tribolium brevicornis r... 33 1.5
gi|4235644 (AF119040) polyprotein [Lycopersicon esculentum] 32 2.6
gi|102939|pir||B36329 hypothetical protein 2 - cabbage looper tr... 31 4.4
gi|1226168 (M32662) ORF B (bases 1850-5560) first start codon at... 31 4.4
gi|130405|sp|P04323|POL3_DROME RETROVIRUS-RELATED POL POLYPROTEI... 31 7.6
>gi|130593|sp|P05400|POL_CERV ENZYMATIC POLYPROTEIN [CONTAINS: ASPARTIC PROTEASE ; ENDONUCLEASE;
REVERSE TRANSCRIPTASE ] >gi|76775|pir||S00854
hypothetical protein 5 - carnation etched ring virus
>gi|58863|emb|CAA28360| (X04658) pot. ORF 5 (AA 1-659)
[Carnation etched ring virus] >gi|225356|prf||1301227E
ORF 5 [Carnation etched ring virus]
Length = 659
Score = 37.9 bits (86), Expect = 0.045
Identities = 27/85 (31%), Positives = 43/85 (49%), Gaps = 18/85 (21%)
Query: 365 IHKSNETVHKYSI------------IEK*FYVIYKTIEIFKILFSCVNIIISADNKNLIS 222
IH S+E + +Y+ EK + + I+ F I + +I DNKN
Sbjct: 548 IHNSHEYICRYASGSFKAAERNYHSNEKELLAVIRVIKKFSIYLTPSRFLIRTDNKNFTH 607
Query: 221 LVKTHLQ------KIAKWEEHLISYDISV*YIKRTHNTSVTFL 111
V +L+ ++ +W+ L YD V +I T N FL
Sbjct: 608 FVNINLKGDRKQGRLVRWQMWLSQYDFDVEHIAGTKNVFADFL 650
>gi|2791289|emb|CAA04050| (AJ000387) protease [Drosophila melanogaster]
Length = 1217
Score = 33.2 bits (74), Expect = 1.1
Identities = 23/75 (30%), Positives = 36/75 (47%), Gaps = 2/75 (2%)
Query: 353 NETVHKYSIIEK*FYVIYKTIEIFKILFSCVNIIISADNKNLISLV--KTHLQKIAKWEE 180
++T YS IEK I ++ F+ V I D+K LI L+ K KI +W
Sbjct: 651 SDTEVNYSTIEKEMLAIIWAVKYFRPYIYGVKFTIVTDHKPLIWLMNFKEPNSKIIRWRL 710
Query: 179 HLISYDISV*YIKRTHN 129
L+ Y+ + + K + N
Sbjct: 711 QLMEYNFEIIHKKGSQN 727
>gi|2147955|pir||S68349 pol polyprotein - Tribolium freemani retrotransposon Woot
(fragment) >gi|1117950 (U40764) polyprotein [Tribolium
freemani]
Length = 190
Score = 32.8 bits (73), Expect = 1.5
Identities = 26/107 (24%), Positives = 47/107 (43%), Gaps = 11/107 (10%)
Query: 335 YSIIEK*FYVIYKTIEIFKILFSCVNIIISADNKNLISLVKTHL--QKIAKWEEHLISYD 162
Y+ EK + + F+I III D++ L L + L +++ +W L YD
Sbjct: 64 YTTTEKELLGVIFALHKFRIYIQATKIIIRTDHQALKFLSRCRLLSERLTRWTLILGQYD 123
Query: 161 ISV*YIKRTHNTSVTFLPKY---------FTPEGNIPTLLEY*NGTKLILIRNDMNFH 15
+ +K N L +Y + E I + E N +++ + D+ H
Sbjct: 124 YEIELVKGKDNVVADILSRYPSDGEASYEYPREQPIVAMFEVHNTPEILQLMTDLKQH 181
>gi|2147954|pir||S68350 pol polyprotein - Tribolium brevicornis retrotransposon Woot
(fragment) >gi|1117948 (U40765) polyprotein [Tribolium
brevicornis]
Length = 203
Score = 32.8 bits (73), Expect = 1.5
Identities = 25/107 (23%), Positives = 48/107 (44%), Gaps = 11/107 (10%)
Query: 335 YSIIEK*FYVIYKTIEIFKILFSCVNIIISADNKNLISLVKTHL--QKIAKWEEHLISYD 162
Y+ EK + + F+I I+I D++ L L + L +++ +W L YD
Sbjct: 67 YTTTEKELLGVIFALHKFRIYIQATKIVIRTDHQALRFLGRCRLLSERLTRWTLLLGQYD 126
Query: 161 ISV*YIKRTHNTSVTFLPKY---------FTPEGNIPTLLEY*NGTKLILIRNDMNFH 15
+ +K N + L +Y + E I + E N +++ + D+ H
Sbjct: 127 YEIELVKGKDNVAADILSRYPSDGDASYEYPREQPIVAMFEVHNTPEILQLLRDLKQH 184
>gi|4235644 (AF119040) polyprotein [Lycopersicon esculentum]
Length = 1542
Score = 32.1 bits (71), Expect = 2.6
Identities = 18/77 (23%), Positives = 32/77 (41%), Gaps = 2/77 (2%)
Query: 359 KSNETVHKYSIIEK*FYVIYKTIEIFKILFSCVNIIISADN--KNLISLVKTHLQKIAKW 186
K N+ +YS EK + ++++++ ++ DN K K A+W
Sbjct: 936 KLNDAEQRYSTHEKEMVAVVHCLQVWRVYLLGTRFVVRTDNVANTFFKTQKKLSPKQARW 995
Query: 185 EEHLISYDISV*YIKRTHN 129
+E L YD + HN
Sbjct: 996 QEFLAEYDFMWEHKPGKHN 1014
>gi|102939|pir||B36329 hypothetical protein 2 - cabbage looper transposon TED (fragment)
Length = 1236
Score = 31.3 bits (69), Expect = 4.4
Identities = 25/83 (30%), Positives = 36/83 (43%), Gaps = 2/83 (2%)
Query: 353 NETVHKYSIIEK*FYVIYKTIEIFKILFSCVNIIISADNKNL--ISLVKTHLQKIAKWEE 180
NE+ YS IEK I + F+ I D+K L + +K ++ +W
Sbjct: 625 NESELNYSTIEKELLAIVWATKYFRPYLFGRKFKILTDHKPLQWMMNLKDPNSRMTRWRL 684
Query: 179 HLISYDISV*YIKRTHNTSVTFLPK 105
L YD SV Y K NT+ L +
Sbjct: 685 RLSEYDFSVVYKKGKSNTNADALSR 709
>gi|1226168 (M32662) ORF B (bases 1850-5560) first start codon at 2306
[Autographa californica nucleopolyhedrovirus]
Length = 1084
Score = 31.3 bits (69), Expect = 4.4
Identities = 25/83 (30%), Positives = 36/83 (43%), Gaps = 2/83 (2%)
Query: 353 NETVHKYSIIEK*FYVIYKTIEIFKILFSCVNIIISADNKNL--ISLVKTHLQKIAKWEE 180
NE+ YS IEK I + F+ I D+K L + +K ++ +W
Sbjct: 473 NESELNYSTIEKELLAIVWATKYFRPYLFGRKFKILTDHKPLQWMMNLKDPNSRMTRWRL 532
Query: 179 HLISYDISV*YIKRTHNTSVTFLPK 105
L YD SV Y K NT+ L +
Sbjct: 533 RLSEYDFSVVYKKGKSNTNADALSR 557
>gi|130405|sp|P04323|POL3_DROME RETROVIRUS-RELATED POL POLYPROTEIN FROM TRANSPOSON 17.6 [CONTAINS:
PROTEASE ; REVERSE TRANSCRIPTASE ; ENDONUCLEASE]
>gi|74642|pir||GNFF17 retrovirus-related pol polyprotein
- fruit fly (Drosophila melanogaster) transposon 17.6
>gi|1335613|emb|CAA25702| (X01472) ORF 2, pot. reverse
transcriptase [Drosophila melanogaster]
>gi|224319|prf||1101404B ORF 2 [Drosophila melanogaster]
Length = 1058
Score = 30.5 bits (67), Expect = 7.6
Identities = 23/75 (30%), Positives = 34/75 (44%), Gaps = 2/75 (2%)
Query: 353 NETVHKYSIIEK*FYVIYKTIEIFKILFSCVNIIISADNKNLISL--VKTHLQKIAKWEE 180
NE YS IEK I + F+ + IS+D++ L L +K K+ +W
Sbjct: 539 NEHEINYSTIEKELLAIVWATKTFRHYLLGRHFEISSDHQPLSWLYRMKDPNSKLTRWRV 598
Query: 179 HLISYDISV*YIKRTHN 129
L +D + YIK N
Sbjct: 599 KLSEFDFDIKYIKGKEN 615
Database: Non-redundant GenBank CDS
translations+PDB+SwissProt+SPupdate+PIR
Posted date: Apr 17, 1999 5:33 AM
Number of letters in database: 113,023,754
Number of sequences in database: 369,800
Lambda K H
0.318 0.135 0.00
Gapped
Lambda K H
0.270 0.0470 4.94e-324
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 93351228
Number of Sequences: 369800
Number of extensions: 1798111
Number of successful extensions: 3941
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 3940
Number of HSP's gapped (non-prelim): 8
length of query: 164
length of database: 113023754
effective HSP length: 52
effective length of query: 111
effective length of database: 93794154
effective search space: 10411151094
effective search space used: 10411151094
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 66 (30.1 bits)