BLASTX 2.0.8 [Jan-05-1999]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 12VII-25R
         (721 letters)

Database: Non-redundant GenBank CDS
translations+PDB+SwissProt+SPupdate+PIR
           368,476 sequences; 112,640,273 total letters

Searching...................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gi|1176864|sp|P45800|YRFF_ECOLI HYPOTHETICAL 79.5 KD PROTEIN IN ...    31  9.6


>gi|1176864|sp|P45800|YRFF_ECOLI HYPOTHETICAL 79.5 KD PROTEIN IN MRCA-PCKA INTERGENIC REGION (O711)
           >gi|606332 (U18997) ORF_o711 [Escherichia coli]
           >gi|1789801 (AE000415) putative dehydrogenase
           [Escherichia coli]
           Length = 711
           
 Score = 30.9 bits (68), Expect = 9.6
 Identities = 15/44 (34%), Positives = 30/44 (68%), Gaps = 7/44 (15%)

Query: 415 PLKVLLSWILLSEFLRAST--NHSNAGTNLSN-----GSGLCGVSTAATWN 546
           PLK  LSW+  ++ + A++    ++AG  + +     G+G+C + T+ TW+
Sbjct: 363 PLKFTLSWMKGAQTIEATSVKQLADAGVRVGDTLRISGTGMCNIRTSGTWS 413


  Database: Non-redundant GenBank CDS
  translations+PDB+SwissProt+SPupdate+PIR
    Posted date:  Apr 12, 1999 12:54 PM
  Number of letters in database: 112,640,273
  Number of sequences in database:  368,476
  
Lambda     K      H
   0.318    0.135     0.00 

Gapped
Lambda     K      H
   0.270   0.0470 4.94e-324 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 136521021
Number of Sequences: 368476
Number of extensions: 2611124
Number of successful extensions: 7210
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 7210
Number of HSP's gapped (non-prelim): 1
length of query: 240
length of database: 112640273
effective HSP length: 53
effective length of query: 186
effective length of database: 93111045
effective search space: 17318654370
effective search space used: 17318654370
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 68 (30.9 bits)