BLASTX 2.0.8 [Jan-05-1999] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 11VII27F (731 letters) Database: Non-redundant GenBank CDS translations+PDB+SwissProt+SPupdate+PIR 368,476 sequences; 112,640,273 total letters Searching...................................................done Score E Sequences producing significant alignments: (bits) Value gi|2983376 (AE000709) flagellar protein FlgA [Aquifex aeolicus] 37 0.17 gi|1707895|sp|P52306|GDS1_HUMAN RAP1 GTPASE-GDP DISSOCIATION STI... 31 9.8
>gi|2983376 (AE000709) flagellar protein FlgA [Aquifex aeolicus] Length = 223 Score = 36.7 bits (83), Expect = 0.17 Identities = 26/86 (30%), Positives = 44/86 (50%), Gaps = 5/86 (5%) Query: 327 YDYMFLFLILTFQLKMDSIVEAEIREIIKDKSSFYNYKGPILKEFEDNTSDFCIFDYKCL 148 Y FLF + TF L ++S+++ E+R+ ++ NYK +L DN S + Sbjct: 21 YAVNFLFFLFTFSLDLESLIKNELRKEFGEEVRLKNYK--VLTGIPDNFSK--------V 70 Query: 147 EIKLXKN-----IEILPTLVGDCVKIIGFKC 70 E+K+ KN + + T VG + +KC Sbjct: 71 ELKVYKNSPRGFLHLKNTKVGTIALELEWKC 101
>gi|1707895|sp|P52306|GDS1_HUMAN RAP1 GTPASE-GDP DISSOCIATION STIMULATOR 1 (SMG P21 STIMULATORY GDP/GTP EXCHANGE PROTEIN) (SMG GDS PROTEIN) >gi|2136175|pir||I37456 smg GDS - human >gi|32080|emb|CAA45067| (X63465) smg GDS [Homo sapiens] Length = 558 Score = 30.9 bits (68), Expect = 9.8 Identities = 19/52 (36%), Positives = 33/52 (62%), Gaps = 1/52 (1%) Query: 165 FDYKCLEIKLXKNIEILPTLVGDCVKIIGFKCNNNSLSKLC-INFKNLYCLKS 10 +D L+ +L N+ ++PTLV K++G C N +L+++C + F NL L+S Sbjct: 118 YDSHSLQAQLI-NMGVIPTLV----KLLGIHCQNAALTEMCLVAFGNLAELES 165 Database: Non-redundant GenBank CDS translations+PDB+SwissProt+SPupdate+PIR Posted date: Apr 12, 1999 12:54 PM Number of letters in database: 112,640,273 Number of sequences in database: 368,476 Lambda K H 0.318 0.135 0.00 Gapped Lambda K H 0.270 0.0470 4.94e-324 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 104322517 Number of Sequences: 368476 Number of extensions: 1699003 Number of successful extensions: 3460 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 1 Number of HSP's successfully gapped in prelim test: 1 Number of HSP's that attempted gapping in prelim test: 3459 Number of HSP's gapped (non-prelim): 2 length of query: 243 length of database: 112640273 effective HSP length: 53 effective length of query: 190 effective length of database: 93111045 effective search space: 17691098550 effective search space used: 17691098550 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.8 bits) X3: 64 (24.9 bits) S1: 41 (21.7 bits) S2: 68 (30.9 bits)