BLASTX 2.0.8 [Jan-05-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 11VII20R
(863 letters)
Database: Non-redundant GenBank CDS
translations+PDB+SwissProt+SPupdate+PIR
369,800 sequences; 113,023,754 total letters
Searching...................................................done
Score E
Sequences producing significant alignments: (bits) Value
gi|103353|pir||A32713 reverse transcriptase homolog - fruit fly ... 41 2e-04
gi|157344 (M17214) reverse transcriptase (put.); putative [Droso... 41 2e-04
gi|103221|pir||S13329 hypothetical protein 2 - fruit fly (Drosop... 37 0.16
gi|8823|emb|CAA35587| (X17551) ORF2 for putative reverse transcr... 37 0.16
gi|134083|sp|P21328|RTJK_DROME RNA-DIRECTED DNA POLYMERASE FROM ... 36 0.47
gi|3880735|emb|CAA21592| (AL032632) Y11D7A.13 [Caenorhabditis el... 34 1.8
gi|1362627|pir||S55543 reverse transcriptase - fruit fly (Drosop... 33 3.1
gi|2133713|pir||S55544 reverse transcriptase - fruit fly (Drosop... 33 3.1
gi|133607|sp|P27176|RRPL_PUUMH RNA-DIRECTED RNA POLYMERASE (L PR... 32 5.4
gi|3282366 (AF012049) reverse transcriptase [Drosophila yakuba] 32 5.4
>gi|103353|pir||A32713 reverse transcriptase homolog - fruit fly (Drosophila melanogaster)
Length = 859
Score = 40.6 bits (93), Expect = 2e-04
Identities = 23/72 (31%), Positives = 39/72 (53%), Gaps = 1/72 (1%)
Query: 171 KVQIVKSVLVPIATYGAKLYG-ISASRVSPLQRVMDNAVRTILSAPSNYCRQAAFSELGL 347
KV + S+L PI TYG++L+G S S + +QR +RTI AP + +L +
Sbjct: 785 KVLLYNSILKPIWTYGSQLWGNASNSNIDIIQRAQSKILRTITGAPWYVRSENIQRDLNI 844
Query: 348 ESIQPGVAKLRTR 386
S+ + +L+ +
Sbjct: 845 PSVTNAITELKEK 857
Score = 25.4 bits (54), Expect = 2e-04
Identities = 13/36 (36%), Positives = 17/36 (47%)
Query: 7 NGSPLPVVDRYTYLGTPFTQTLCLDAIIEDRKEKAR 114
N PLP D TYLG + L IE +K + +
Sbjct: 729 NSIPLPKADEVTYLGVHLDRRLTWRRHIEAKKTQLK 764
>gi|157344 (M17214) reverse transcriptase (put.); putative [Drosophila
melanogaster]
Length = 858
Score = 40.6 bits (93), Expect = 2e-04
Identities = 23/72 (31%), Positives = 39/72 (53%), Gaps = 1/72 (1%)
Query: 171 KVQIVKSVLVPIATYGAKLYG-ISASRVSPLQRVMDNAVRTILSAPSNYCRQAAFSELGL 347
KV + S+L PI TYG++L+G S S + +QR +RTI AP + +L +
Sbjct: 784 KVLLYNSILKPIWTYGSQLWGNASNSNIDIIQRAQSKILRTITGAPWYVRSENIQRDLNI 843
Query: 348 ESIQPGVAKLRTR 386
S+ + +L+ +
Sbjct: 844 PSVTNAITELKEK 856
Score = 25.4 bits (54), Expect = 2e-04
Identities = 13/36 (36%), Positives = 17/36 (47%)
Query: 7 NGSPLPVVDRYTYLGTPFTQTLCLDAIIEDRKEKAR 114
N PLP D TYLG + L IE +K + +
Sbjct: 728 NSIPLPKADEVTYLGVHLDRRLTWRRHIEAKKTQLK 763
>gi|103221|pir||S13329 hypothetical protein 2 - fruit fly (Drosophila melanogaster)
Length = 964
Score = 37.1 bits (84), Expect = 0.16
Identities = 23/80 (28%), Positives = 41/80 (50%), Gaps = 1/80 (1%)
Query: 147 RKNVPVPVKVQIVKSVLVPIATYGAKLYG-ISASRVSPLQRVMDNAVRTILSAPSNYCRQ 323
R + + K + SVL PI TYG++L+G S S + +QR +R I AP +
Sbjct: 850 RSPLSLEFKALLYNSVLKPIWTYGSELWGNASRSNIDIIQRAQSRILRIITGAPWYLRNE 909
Query: 324 AAFSELGLESIQPGVAKLRTR 386
+L ++ + +A+ +T+
Sbjct: 910 NIHRDLKIKLVIEVIAEKKTK 930
>gi|8823|emb|CAA35587| (X17551) ORF2 for putative reverse transcriptase [Drosophila
melanogaster]
Length = 888
Score = 37.1 bits (84), Expect = 0.16
Identities = 23/80 (28%), Positives = 41/80 (50%), Gaps = 1/80 (1%)
Query: 147 RKNVPVPVKVQIVKSVLVPIATYGAKLYG-ISASRVSPLQRVMDNAVRTILSAPSNYCRQ 323
R + + K + SVL PI TYG++L+G S S + +QR +R I AP +
Sbjct: 774 RSPLSLEFKALLYNSVLKPIWTYGSELWGNASRSNIDIIQRAQSRILRIITGAPWYLRNE 833
Query: 324 AAFSELGLESIQPGVAKLRTR 386
+L ++ + +A+ +T+
Sbjct: 834 NIHRDLKIKLVIEVIAEKKTK 854
>gi|134083|sp|P21328|RTJK_DROME RNA-DIRECTED DNA POLYMERASE FROM MOBILE ELEMENT JOCKEY (REVERSE
TRANSCRIPTASE) >gi|85128|pir||JT0396 reverse
transcriptase homolog - fruit fly (Drosophila
melanogaster) transposon jockey >gi|157825 (M22874)
ORF2, reverse transcriptase (put.); putative [Drosophila
melanogaster]
Length = 916
Score = 35.6 bits (80), Expect = 0.47
Identities = 19/53 (35%), Positives = 31/53 (57%), Gaps = 1/53 (1%)
Query: 147 RKNVPVPVKVQIVKSVLVPIATYGAKLYGISA-SRVSPLQRVMDNAVRTILSAP 305
R + + KV I KS+L P YG ++YGI+A S ++ ++ + +R I AP
Sbjct: 788 RNKLSLGCKVNIYKSILAPCLFYGLQVYGIAAKSHLNKIRILQAKTLRRISGAP 841
>gi|3880735|emb|CAA21592| (AL032632) Y11D7A.13 [Caenorhabditis elegans]
Length = 440
Score = 33.6 bits (75), Expect = 1.8
Identities = 24/82 (29%), Positives = 46/82 (55%), Gaps = 12/82 (14%)
Query: 661 LAANSLGGIRRILPPSLILPMTDLSRET-----------SLFLITSAINSTFL*SGTRDS 515
L+ N + +LPPSL+ P +DL ET ++ ++ S N+ G +
Sbjct: 219 LSLNGPQQTKNLLPPSLLTPKSDLEDETLSTKTEQKNASTMNILLSMTNNC---DGRKPI 275
Query: 514 STACPNLFSHLADPETHVAHLVLGKASI-NKEIQ 416
+ P+LF+ +++ E H + L K + N+E++
Sbjct: 276 TLRLPDLFNKISEMEIHDMEMTLTKFLLENRELR 309
>gi|1362627|pir||S55543 reverse transcriptase - fruit fly (Drosophila melanogaster)
Length = 860
Score = 32.8 bits (73), Expect = 3.1
Identities = 22/82 (26%), Positives = 43/82 (51%), Gaps = 2/82 (2%)
Query: 141 LNRKN-VPVPVKVQIVKSVLVPIATYGAKLYGISA-SRVSPLQRVMDNAVRTILSAPSNY 314
LNRK+ + + K + + PI YG +++GI+A S + +Q + + A+R I P
Sbjct: 740 LNRKSTMTLRAKRAVYVHCVAPIWLYGIQIWGIAAKSNYNRIQVLQNRAMRAITDCPYYV 799
Query: 315 CRQAAFSELGLESIQPGVAKLRTR 386
+L L +++ ++K +R
Sbjct: 800 RGTTLHRDLNLHTVEEQISKHTSR 823
>gi|2133713|pir||S55544 reverse transcriptase - fruit fly (Drosophila melanogaster)
(fragment)
Length = 804
Score = 32.8 bits (73), Expect = 3.1
Identities = 22/82 (26%), Positives = 43/82 (51%), Gaps = 2/82 (2%)
Query: 141 LNRKN-VPVPVKVQIVKSVLVPIATYGAKLYGISA-SRVSPLQRVMDNAVRTILSAPSNY 314
LNRK+ + + K + + PI YG +++GI+A S + +Q + + A+R I P
Sbjct: 684 LNRKSTMTLRAKRAVYVHCVAPIWLYGIQIWGIAAKSNYNRIQVLQNRAMRAITDCPYYV 743
Query: 315 CRQAAFSELGLESIQPGVAKLRTR 386
+L L +++ ++K +R
Sbjct: 744 RGTTLHRDLNLHTVEEQISKHTSR 767
>gi|133607|sp|P27176|RRPL_PUUMH RNA-DIRECTED RNA POLYMERASE (L PROTEIN) >gi|67086|pir||RRVUNE
RNA-directed RNA polymerase (EC 2.7.7.48) - Puumala virus
(strain Hallnas B1)
Length = 2156
Score = 32.1 bits (71), Expect = 5.4
Identities = 23/77 (29%), Positives = 41/77 (52%), Gaps = 4/77 (5%)
Query: 240 ASRVSPLQRVMDNAVRT-ILSAPSNYCRQAAFSELGLESIQPGVAKLRTRDFLKWKNSRT 416
AS+V + V+D+A+ LS R+ + ELGL+ QP V K L +K+ ++
Sbjct: 1512 ASKVDEISDVLDSAIHPDSLSTDLQLMREGVYRELGLDISQPNVLKKVAP--LLYKSGKS 1569
Query: 417 WISLL---IEAFPRTRCATWV 470
I ++ +E + C+ W+
Sbjct: 1570 RIVIVQGNVEGTAESICSYWL 1590
>gi|3282366 (AF012049) reverse transcriptase [Drosophila yakuba]
Length = 439
Score = 32.1 bits (71), Expect = 5.4
Identities = 20/82 (24%), Positives = 43/82 (52%), Gaps = 2/82 (2%)
Query: 141 LNRKN-VPVPVKVQIVKSVLVPIATYGAKLYGISA-SRVSPLQRVMDNAVRTILSAPSNY 314
+NR+N + + K + ++PI YG +++GI+A S +Q + + A+R I + P
Sbjct: 334 MNRRNTLSLRCKRAVYAHCILPIWLYGIQIWGIAAKSNYKRIQVMQNRALRQITNCPWYV 393
Query: 315 CRQAAFSELGLESIQPGVAKLRTR 386
+L L +++ + + +R
Sbjct: 394 RNSTLHKDLNLYTVEEQIGRHTSR 417
Database: Non-redundant GenBank CDS
translations+PDB+SwissProt+SPupdate+PIR
Posted date: Apr 17, 1999 5:33 AM
Number of letters in database: 113,023,754
Number of sequences in database: 369,800
Lambda K H
0.318 0.135 0.00
Gapped
Lambda K H
0.270 0.0470 4.94e-324
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 179197908
Number of Sequences: 369800
Number of extensions: 3695883
Number of successful extensions: 8352
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 9
Number of HSP's that attempted gapping in prelim test: 8347
Number of HSP's gapped (non-prelim): 12
length of query: 287
length of database: 113023754
effective HSP length: 54
effective length of query: 233
effective length of database: 93054554
effective search space: 21681711082
effective search space used: 21681711082
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 69 (31.3 bits)