BLASTX 2.0.8 [Jan-05-1999] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 11VII-27F (486 letters) Database: Non-redundant GenBank CDS translations+PDB+SwissProt+SPupdate+PIR 368,476 sequences; 112,640,273 total letters Searching...................................................done Score E Sequences producing significant alignments: (bits) Value gi|2983376 (AE000709) flagellar protein FlgA [Aquifex aeolicus] 36 0.13 gi|1171858|sp|P21301|NUCM_TRYBB NADH-UBIQUINONE OXIDOREDUCTASE 4... 31 5.7 gi|1707895|sp|P52306|GDS1_HUMAN RAP1 GTPASE-GDP DISSOCIATION STI... 30 9.7
>gi|2983376 (AE000709) flagellar protein FlgA [Aquifex aeolicus] Length = 223 Score = 36.4 bits (82), Expect = 0.13 Identities = 26/86 (30%), Positives = 44/86 (50%), Gaps = 5/86 (5%) Query: 327 YDYMFLFLILTFQLKMDSIVEAEIREIIKDKSSFYNYKGPILKEFEDNTSDFCIFDYKCL 148 Y FLF + TF L ++S+++ E+R+ ++ NYK +L DN S + Sbjct: 21 YAVNFLFFLFTFSLDLESLIKNELRKEFGEEVRLKNYK--VLTGIPDNFSK--------V 70 Query: 147 EIKLKKN-----IEILPTLVGDCVKIIGFKC 70 E+K+ KN + + T VG + +KC Sbjct: 71 ELKVYKNSPRGFLHLKNTKVGTIALELEWKC 101
>gi|1171858|sp|P21301|NUCM_TRYBB NADH-UBIQUINONE OXIDOREDUCTASE 49 KD SUBUNIT HOMOLOG (NADH DEHYDROGENASE SUBUNIT 7 HOMOLOG) >gi|102130|pir||A35693 NADH dehydrogenase (EC 1.6.99.3) chain 7 - Trypanosoma brucei mitochondrion (SGC6) Length = 386 Score = 30.9 bits (68), Expect = 5.7 Identities = 26/113 (23%), Positives = 50/113 (44%), Gaps = 2/113 (1%) Query: 378 MIFKCQ--KCFESYKYISFYDYMFLFLILTFQLKMDSIVEAEIREIIKDKSSFYNYKGPI 205 M++ C+ C+E Y ++ YDY F F+ F + + + +I + F + Sbjct: 229 MVWDCRLFSCYELY-FMFCYDYCFCFIGDAFDRLFLRLFDMRMSLLICKQCFFVGF---F 284 Query: 204 LKEFEDNTSDFCIFDYKCLEIKLKKNIEILPTLVGDCVKIIGFKCNNNSLSKLCI 40 + F C+FDY +I ++ I + +L C+ I F C + + C+ Sbjct: 285 VFGF------VCLFDYLYCDITIETIIMLFYSLWCCCLPGISFACVEHPKGEYCL 333
>gi|1707895|sp|P52306|GDS1_HUMAN RAP1 GTPASE-GDP DISSOCIATION STIMULATOR 1 (SMG P21 STIMULATORY GDP/GTP EXCHANGE PROTEIN) (SMG GDS PROTEIN) >gi|2136175|pir||I37456 smg GDS - human >gi|32080|emb|CAA45067| (X63465) smg GDS [Homo sapiens] Length = 558 Score = 30.1 bits (66), Expect = 9.7 Identities = 16/40 (40%), Positives = 27/40 (67%), Gaps = 1/40 (2%) Query: 129 NIEILPTLVGDCVKIIGFKCNNNSLSKLC-INFKNLYCLKS 10 N+ ++PTLV K++G C N +L+++C + F NL L+S Sbjct: 129 NMGVIPTLV----KLLGIHCQNAALTEMCLVAFGNLAELES 165 Database: Non-redundant GenBank CDS translations+PDB+SwissProt+SPupdate+PIR Posted date: Apr 12, 1999 12:54 PM Number of letters in database: 112,640,273 Number of sequences in database: 368,476 Lambda K H 0.318 0.135 0.00 Gapped Lambda K H 0.270 0.0470 4.94e-324 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 74702871 Number of Sequences: 368476 Number of extensions: 1294487 Number of successful extensions: 3083 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 1 Number of HSP's successfully gapped in prelim test: 2 Number of HSP's that attempted gapping in prelim test: 3082 Number of HSP's gapped (non-prelim): 3 length of query: 162 length of database: 112640273 effective HSP length: 52 effective length of query: 109 effective length of database: 93479521 effective search space: 10189267789 effective search space used: 10189267789 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.8 bits) X3: 64 (24.9 bits) S1: 41 (21.7 bits) S2: 66 (30.1 bits)