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Kasia Hammar
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Education:
1978-1981 Bachelor of Arts in Biology Amherst College
1994 Teacher Certificate in Biology, Grades 9-12 University of Massachusetts, Dartmouth
Academic appointments:
1981-1983 Research Assistant Woods Hole Oceanographic Institution, Woods Hole, MA
Research Activities: Shellfish PCB analysis; Column chromatography
1983-1985 Electron Microscopy Technician VA Medical Center, Boston
Research Activities: Electron microscopy; Morphological study of muscle spindles
1985-1994 Histopathology and Electron Microscopy Technician NIH/NINDS Neurobiology Laboratory, Marine Biological Laboratory
Research Activities: Morphology and immunocytochemical studies of axonal transport in squid; Tissue culture; Neural growth and development of single cells
Laboratory Management: overall organization, supervision, maintenance of equipment, budget and inventory coordination
1994-1998 Research Assistant II NIH/NCRR Nat’l Vibrating Probe Facility/BioCurrents Research Center, Marine Biological Laboratory
Research Activities: Measurements of ion flux across living tissues; Use of the ion selective probe; Analysis and data summation; Tissue culture of Aplysia bag cells; Technical assistant to visiting scientists; Immunocytochemical studies of Heat Shock Protein-70 and calcium imaging
1998-2003 Research Assistant III NIH-NCRR BioCurrents Research Center, Marine Biological Laboratory
Research Activities: Assistant and collaborator to the visiting scientists; Coordinates scientific aspects of each visit, provides technical training, conducts experiments and collaborates in various scientific studies. Microsensor development and production: ion selective electrode testing and making new ionophores, oxygen electrode, glucose and nitric oxide building, testing, characterizing and calibrating, analysis and data summation. Electrophysiological study: oxygen consumption measurements using self-reference technique while voltage clamping single horizontal cell from Skate retina, analysis and data summation, poster preparation. Laboratory Management: overall organization, supervision, maintenance of equipment, budget and inventory coordination. Ratio Imaging: fully responsible for the alignment and upkeep of the Attofluor Ratio Imaging system, calcium, pH and sodium imaging. Tissue culture: Aplysia bag cell, horizontal cell Skate retina, hippocampal neuron and glia preparation, overlooks the tissue culture facility
2004-present Research Assistant III Josephine Bay Paul Center for Comparative Molecular Biology and Evolution
Oversight and operation of high throughput sequencing facility: calibration, maintenance and repair of instruments. Processes samples for internal/external projects, record keeping, supplies inventory. ESTcDNA project: high throughput sequencing and construction of new cDNA libraries, upkeep of culture collection.
Professional memberships:

 

Distinctions and awards:

 

Other activities:

 

Publications:

Kucera, J., Hammar, K. and Meek, B.: Ultrastructure of dynamic and static skeletal fusimotor endings in a cat muscle spindle., Cell and Tissue Research, 238:151-158 (1984).

Gallant, P., Hammar, K. and Reese, T.S.: Cytoplasmic contraction and vesiculation after axotomy of the squid giant axon, J. of Neurocytology, 24 (2):943-954 (1995).

Smith, P.J.S., Hammar, K. and Tytell, M.: Effects of exogenous Heat Shock Protein (hsp70) on neuronal calcium flux, Biological Bulletin, 189:209-210 (1995).

Katoh, K., Langford, G., Hammar, K., Smith, P.J.S. and Oldenbourg, R.: Observation of actin bundles in neural growth cone with the Oldenbourg Pol-Scope, Biological Bulletin, 193:219-220 (1997).

Metuzals, J., Chang, D., Hammar, K. and Reese, T.S.: Organization of the cortical endoplasmic reticulum in the squid giant axon., J. Neurocytology , 26 (8):529-539 (1997).

Breton, S., Hammar, K., Smith, P.J.S. and Brown, D.: Proton secretion in the male reproductive tract: involvement of chloride-independent bicarbonate transport , Am. J. Physiol., 275:C1134-C1142 (1998).

Shirihai, O., Smith, P.J.S., Hammar, K. and Dagan, D.: H+ and K+ gradient generated by microglia H/K ATPase , Glia, 238:339-348 (1998).

Tamse, C.T., Porterfield, D.M., Smith, P.J.S. and Hammar, K.: Transmembrane calcium and oxygen fluxes in Pb-exposed Aplysia neurons, Biol. Bull, 195:201-202 (1998).

Hill, J. L., Hammar,K., Smith, P. J. S. and Gross, D. J.: Stage-dependent effects of epidermal growth factor on Ca2+ efflux in mouse oocytes. , Molec. Reprod. Develop., 53:244-253 (1999).

Katoh, K., Hammar, K., Smith, P.J.S. and Oldenbourg, R.: Arrangement of radial actin bundles in the growth cone of Aplysia bag cell neurons shows a short history of filopodial behavior , Proc. Natl. Acad. Sci., USA, 96:7928-7931 (1999).

Katoh, K., Hammar, K., Smith, P.J.S. and Oldenbourg, R.: Birefringence imaging directly reveals architectural dynamics of filamentous actin in living growth cones, Mol. Biol. Cell, 10:197-210 (1999).

Smith, P.J.S., Hammar, K., Porterfield, D.M., Sanger, R.H. and Trimarchi, J.R.: A self-referencing, non-invasive, ion-selective electrode for single cell detection of trans-plasma membrane calcium flux , Microscopy Research Techniques, 46:398-417 (1999).

Gleeson, R. A., Hammar, K. and Smith, P. J. S.: Sustaining olfaction at low salinities:mapping ion flux associated with the olfactory sensilla of the blue crab Callinectes sapidus, J. Exp. Biol., 203:3145-3152 (2000).

Gleeson, R.A., McDowell, L.M., Aldrich, H.C., Hammar, K. and Smith, P.J.S.: Sustaining olfaction at low salinities: evidence for a paracellular route of ion movement from the hemolymph to the sensillar lymph in the olfactory sensillar of the blue crab, Callinectes sapidus. Cell and Tissue Research, (2000).

Jung, S.-K., Hammar, K. and Smith, P.J.S.: Development of self-referencing oxygen microsensor and its application to single HIT cells, Biological Bulletin, 199 (2):197-198 (2000).

Liu L, Hammar K, Smith PJS, Inoue S, Keefe D.: Mitochondrial modulation of calcium signaling at the initiation of development , Cell Calcium, 30 (6):423-433 (2001).

Twig G, Malchow,RP, Hammar K, Smith PJSS, Levy H, Perlman I.: A novel turtle retinal preparation for simultaneously measuring light-induced electrical activity and changes in metabolite levels, Biological Bulletin, 203:198-200 (2002).

Molina AJ, Hammar K, Sanger R, Smith PJ, Malchow RP: Intracellular release of caged calcium in skate horizontal cells using fine optical fibers, Biological Bulletin, 205(2):215 (2003).

Anthony J. A Molina1, Michael P Verzi2, Andrea D Birnbaum1, Ebenezer N Yamoah3, Katherine Hammar4, Peter J. S Smith4 and Robert Paul Malchow: Neurotransmitter modulation of extracellular H+ fluxes from isolated retinal horizontal cells of the skate, J Physiol., Nov 1;560(Pt 3):639-57 (2004).