Advances In Genome Technology and Bioinformatics |
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| Tuesday, 10/4/2005 | ||||||
| All Day | Registration | Swope | ||||
| 7:00 | Reception | Swope Private Dining Room | ||||
| Wednesday, 10/5/2005 | |||
| 8:30 | Mitchell Sogin | Introduction | Candle House 104-105 |
| 11:00 | Andrew McArthur | Why Command Lines? | Candle House 104-105 |
| 1:00 | Bill Nierman | Genomic DNA library construction | Candle House 104-105 |
| 3:00 | Hilary Morrison/Phil Neal/Susan Huse |
Genomic library preparation: nebulization of fosmid DNA & Intro to Perl Lab Results |
Loeb 323 |
| 7:00 | Hilary Morrison | Genomic Library: Bal31 Treatment, fosmid DNA | Loeb 323 |
| Thursday, 10/6/2005 | |||
| 8:30 | Hilary Morrison, Kasia Hammar | Genomic library: size selection, clean up, ligation into pUC18 cDNA library preparation: First strand cDNA synthesis, second strand synthesis | Loeb 323 |
| 1:00 | Ewen Kirkness |
cDNA library construction Suggested Reading |
CH 104-105 |
| 3:00 | Hilary Morrison, Kasia Hammar |
cDNA library: check cDNA on gel Lab Results |
Loeb 323 |
| 7:00 | Mitchell Sogin/Andrew McArthur |
Computer Lab - Introduction to Unix Files: Linux Demo, Linux Cheat Sheet |
Loeb 323 |
| Friday, 10/7/2005 | |||
| 8:30 | Phillip Neal/Sue Huse | Introduction to Perl Programming | Computer Lab |
| 9:30 | Hilary Morrison, Kasia Hammar | genomic library: Size select ligation products, clean up, transform cells | Loeb 323 |
| 1:00 | Kasia Hammar | cDNA library: proteinaseK treatment | Loeb 323 |
| 3:00 | Kasia Hammar |
cDNA library: SfiI digestion and size fractionation, run gel
Lab results |
Loeb 323 |
| 7:00 | Kasia Hammar | cDNA library: Pool cDNA fractions, ppt overnight | Loeb 323 |
| Saturday, 10/8/2005 | |||
| 8:30 | Phillip Neal/Susan Huse | Introduction to Perl Programming | Computer Lab |
| 10:30 | Hilary Morrison, Kasia Hammar | Genomic library: Pick colonies for template prep cDNA library: Recover cDNA and set up ligation overnight | Loeb 323 |
| Sunday, 10/9/2005 | |||
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Free Day
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